Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium
Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and cong...
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| Veröffentlicht in: | Experimental eye research 1986-10, Vol.43 (4), p.561-573 |
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| creator | Katz, Martin L. Drea, Christine M. Eldred, Graig E. Hess, Helen H. Robison, W. Gerald |
| description | Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscìn-like fluorescence of the outer-segment fragments in the RDY rats. |
| doi_str_mv | 10.1016/S0014-4835(86)80023-9 |
| format | Article |
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Gerald</creator><creatorcontrib>Katz, Martin L. ; Drea, Christine M. ; Eldred, Graig E. ; Hess, Helen H. ; Robison, W. Gerald</creatorcontrib><description>Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscìn-like fluorescence of the outer-segment fragments in the RDY rats.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/S0014-4835(86)80023-9</identifier><identifier>PMID: 3792460</identifier><identifier>CODEN: EXERA6</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Aging ; Animals ; autofluorescence ; Biological and medical sciences ; Eye and associated structures. Visual pathways and centers. Vision ; Female ; Fundamental and applied biological sciences. Psychology ; lipofuscin ; Lipofuscin - metabolism ; Male ; Microscopy, Electron ; Photoreceptor Cells - metabolism ; photoreceptors ; Pigment Epithelium of Eye - metabolism ; Pigment Epithelium of Eye - ultrastructure ; Pigments, Biological - metabolism ; Rats ; Rats, Inbred Strains ; Retina - pathology ; Retina - ultrastructure ; retinal degeneration ; Retinal Degeneration - metabolism ; Retinal Degeneration - pathology ; retinal pigment epithelium ; thin-layer chromatography ; Vertebrates: nervous system and sense organs</subject><ispartof>Experimental eye research, 1986-10, Vol.43 (4), p.561-573</ispartof><rights>1986 Academic Press Inc. (London) Limited</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-e11c25143d6b6adbd5824bdb211b8a06a214831d0e4ccb1aca88b7aab98e90883</citedby><cites>FETCH-LOGICAL-c441t-e11c25143d6b6adbd5824bdb211b8a06a214831d0e4ccb1aca88b7aab98e90883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-4835(86)80023-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7989969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3792460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katz, Martin L.</creatorcontrib><creatorcontrib>Drea, Christine M.</creatorcontrib><creatorcontrib>Eldred, Graig E.</creatorcontrib><creatorcontrib>Hess, Helen H.</creatorcontrib><creatorcontrib>Robison, W. Gerald</creatorcontrib><title>Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscìn-like fluorescence of the outer-segment fragments in the RDY rats.</description><subject>Aging</subject><subject>Animals</subject><subject>autofluorescence</subject><subject>Biological and medical sciences</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>lipofuscin</subject><subject>Lipofuscin - metabolism</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Photoreceptor Cells - metabolism</subject><subject>photoreceptors</subject><subject>Pigment Epithelium of Eye - metabolism</subject><subject>Pigment Epithelium of Eye - ultrastructure</subject><subject>Pigments, Biological - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Retina - pathology</subject><subject>Retina - ultrastructure</subject><subject>retinal degeneration</subject><subject>Retinal Degeneration - metabolism</subject><subject>Retinal Degeneration - pathology</subject><subject>retinal pigment epithelium</subject><subject>thin-layer chromatography</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVoSTZJf0JAh1Cag1vJlmXpFELoRyDQQ5tjEfoYJwqy5Eh2If--2uyy18LAwMw778w8CF1Q8pkSyr_8IoSyhomu_yT4lSCk7Rp5hDaUSN4QQoZ3aHOQnKDTUp5rtWMDO0bH3SBbxskG_bmLY1ghWsBpxKBzeMXzU1pSBgtzTdjBI0TIevEp4hrBz2lci_UR11ieAGdYfNQBz_5xgrhgmH0tB79O5-j9qEOBD_t8hh6-ff19-6O5__n97vbmvrGM0aUBSm3bU9Y5brh2xvWiZcaZllIjNOG6pfUJ6ggwaw3VVgthBq2NFCCJEN0Z-rjznXN6WaEsavLFQgg6QlqLGoZq1bddFfY7oc2plAyjmrOfdH5VlKgtVvWGVW2ZKcHVG1Yl69zFfsFqJnCHqT3H2r_c93WxOoxZR-vLQTZIISXf2lzvZFBh_PWQVeW4he985b0ol_x_DvkHUD6VvQ</recordid><startdate>19861001</startdate><enddate>19861001</enddate><creator>Katz, Martin L.</creator><creator>Drea, Christine M.</creator><creator>Eldred, Graig E.</creator><creator>Hess, Helen H.</creator><creator>Robison, W. Gerald</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861001</creationdate><title>Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium</title><author>Katz, Martin L. ; Drea, Christine M. ; Eldred, Graig E. ; Hess, Helen H. ; Robison, W. Gerald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-e11c25143d6b6adbd5824bdb211b8a06a214831d0e4ccb1aca88b7aab98e90883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aging</topic><topic>Animals</topic><topic>autofluorescence</topic><topic>Biological and medical sciences</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>lipofuscin</topic><topic>Lipofuscin - metabolism</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Photoreceptor Cells - metabolism</topic><topic>photoreceptors</topic><topic>Pigment Epithelium of Eye - metabolism</topic><topic>Pigment Epithelium of Eye - ultrastructure</topic><topic>Pigments, Biological - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Retina - pathology</topic><topic>Retina - ultrastructure</topic><topic>retinal degeneration</topic><topic>Retinal Degeneration - metabolism</topic><topic>Retinal Degeneration - pathology</topic><topic>retinal pigment epithelium</topic><topic>thin-layer chromatography</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katz, Martin L.</creatorcontrib><creatorcontrib>Drea, Christine M.</creatorcontrib><creatorcontrib>Eldred, Graig E.</creatorcontrib><creatorcontrib>Hess, Helen H.</creatorcontrib><creatorcontrib>Robison, W. Gerald</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katz, Martin L.</au><au>Drea, Christine M.</au><au>Eldred, Graig E.</au><au>Hess, Helen H.</au><au>Robison, W. Gerald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>1986-10-01</date><risdate>1986</risdate><volume>43</volume><issue>4</issue><spage>561</spage><epage>573</epage><pages>561-573</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><coden>EXERA6</coden><abstract>Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscìn-like fluorescence of the outer-segment fragments in the RDY rats.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>3792460</pmid><doi>10.1016/S0014-4835(86)80023-9</doi><tpages>13</tpages></addata></record> |
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| subjects | Aging Animals autofluorescence Biological and medical sciences Eye and associated structures. Visual pathways and centers. Vision Female Fundamental and applied biological sciences. Psychology lipofuscin Lipofuscin - metabolism Male Microscopy, Electron Photoreceptor Cells - metabolism photoreceptors Pigment Epithelium of Eye - metabolism Pigment Epithelium of Eye - ultrastructure Pigments, Biological - metabolism Rats Rats, Inbred Strains Retina - pathology Retina - ultrastructure retinal degeneration Retinal Degeneration - metabolism Retinal Degeneration - pathology retinal pigment epithelium thin-layer chromatography Vertebrates: nervous system and sense organs |
| title | Influence of early photoreceptor degeneration on lipofuscin in the retinal pigment epithelium |
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