Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions
In an attempt to determine whether the fluorescent in situ hybridization (FISH) can be used as a rapid approach for the identification of aneuploidy in premalignant cervical smears, a centromeric probe for chromosome 1 was used. The results from the FISH experiments were compared with measurements o...
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| Veröffentlicht in: | The Histochemical journal 1995-01, Vol.27 (1), p.24-34 |
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| container_title | The Histochemical journal |
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| creator | Segers, P Haesen, S Castelain, P Amy, J J De Sutter, P Van Dam, P Kirsch-Volders, M |
| description | In an attempt to determine whether the fluorescent in situ hybridization (FISH) can be used as a rapid approach for the identification of aneuploidy in premalignant cervical smears, a centromeric probe for chromosome 1 was used. The results from the FISH experiments were compared with measurements of the overall DNA content obtained by means of an image analysis system. With progression to neoplasia, a decrease of the frequency of cells with two spots was observed, due to an increasing polysomy of chromosome 1. As far as the DNA content was concerned, an increasing DNA index and 5C-exceeding ratio (fraction of cells with a DNA content higher than 5C) was observed. Classification of the FISH results by a linear discriminant analysis revealed that 67.6% of the cases were classified in agreement with the CIN classification. These data suggest that chromosome 1 may be considered as a marker chromosome for pre-malignant cervical lesions and that the DNA content measurements are complementary to the FISH results. |
| doi_str_mv | 10.1007/bf00164169 |
| format | Article |
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These data suggest that chromosome 1 may be considered as a marker chromosome for pre-malignant cervical lesions and that the DNA content measurements are complementary to the FISH results.</description><identifier>ISSN: 0018-2214</identifier><identifier>EISSN: 1573-6865</identifier><identifier>DOI: 10.1007/bf00164169</identifier><identifier>PMID: 7713754</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Adult ; Aneuploidy ; Cervical Intraepithelial Neoplasia - genetics ; Cervical Intraepithelial Neoplasia - pathology ; Chromosome Aberrations ; Chromosomes, Human, Pair 1 ; Densitometry - instrumentation ; DNA, Neoplasm ; False Negative Reactions ; False Positive Reactions ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Lymphocytes - cytology ; Neoplasm Staging ; Uterine Cervical Dysplasia - genetics ; Uterine Cervical Dysplasia - pathology ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology ; Vaginal Smears</subject><ispartof>The Histochemical journal, 1995-01, Vol.27 (1), p.24-34</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-4bf3a7d86f53b421bd6f2b3c7d612366229135bf0d1f9a8c2248f1cc9cfebaa23</citedby><cites>FETCH-LOGICAL-c292t-4bf3a7d86f53b421bd6f2b3c7d612366229135bf0d1f9a8c2248f1cc9cfebaa23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7713754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Segers, P</creatorcontrib><creatorcontrib>Haesen, S</creatorcontrib><creatorcontrib>Castelain, P</creatorcontrib><creatorcontrib>Amy, J J</creatorcontrib><creatorcontrib>De Sutter, P</creatorcontrib><creatorcontrib>Van Dam, P</creatorcontrib><creatorcontrib>Kirsch-Volders, M</creatorcontrib><title>Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions</title><title>The Histochemical journal</title><addtitle>Histochem J</addtitle><description>In an attempt to determine whether the fluorescent in situ hybridization (FISH) can be used as a rapid approach for the identification of aneuploidy in premalignant cervical smears, a centromeric probe for chromosome 1 was used. The results from the FISH experiments were compared with measurements of the overall DNA content obtained by means of an image analysis system. With progression to neoplasia, a decrease of the frequency of cells with two spots was observed, due to an increasing polysomy of chromosome 1. As far as the DNA content was concerned, an increasing DNA index and 5C-exceeding ratio (fraction of cells with a DNA content higher than 5C) was observed. Classification of the FISH results by a linear discriminant analysis revealed that 67.6% of the cases were classified in agreement with the CIN classification. These data suggest that chromosome 1 may be considered as a marker chromosome for pre-malignant cervical lesions and that the DNA content measurements are complementary to the FISH results.</description><subject>Adult</subject><subject>Aneuploidy</subject><subject>Cervical Intraepithelial Neoplasia - genetics</subject><subject>Cervical Intraepithelial Neoplasia - pathology</subject><subject>Chromosome Aberrations</subject><subject>Chromosomes, Human, Pair 1</subject><subject>Densitometry - instrumentation</subject><subject>DNA, Neoplasm</subject><subject>False Negative Reactions</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Lymphocytes - cytology</subject><subject>Neoplasm Staging</subject><subject>Uterine Cervical Dysplasia - genetics</subject><subject>Uterine Cervical Dysplasia - pathology</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Vaginal Smears</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kclKxTAUhoMoeh027oWsRIVqhjZpl84Kogt1XTJqpE2uSSvUt_FNzdWrqwPnfOc_ww_ALkbHGCF-Ii1CmJWYNStghitOC1azahXMcrouCMHlBthM6Q0h1HDO1sE655jyqpyBr8dh1BMMFvqxN9Ep0UEhTYxicMGnRUG9xtCHFHoDMZQTtN0YoknK-AE6D5MbRvg6yei0-_zpgsJreHF_ClXww4LKTdr4DGaNIc_IgOim5LK8hwfzaA6LXnTuxYsMKxM_ftboTFqssA3WrOiS2VnGLfB8dfl0flPcPVzfnp_eFYo0ZChKaanguma2orIkWGpmiaSKa4YJZYyQBtMqf0pj24haEVLWFivVKGukEIRugf1f3XkM76NJQ9u7fGTXCW_CmFrOCcaIswwe_YIqhpSise08ul7EqcWoXfjRnl39-ZHhvaXqKHuj_9GlAfQbAVCJIQ</recordid><startdate>199501</startdate><enddate>199501</enddate><creator>Segers, P</creator><creator>Haesen, S</creator><creator>Castelain, P</creator><creator>Amy, J J</creator><creator>De Sutter, P</creator><creator>Van Dam, P</creator><creator>Kirsch-Volders, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199501</creationdate><title>Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions</title><author>Segers, P ; 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The results from the FISH experiments were compared with measurements of the overall DNA content obtained by means of an image analysis system. With progression to neoplasia, a decrease of the frequency of cells with two spots was observed, due to an increasing polysomy of chromosome 1. As far as the DNA content was concerned, an increasing DNA index and 5C-exceeding ratio (fraction of cells with a DNA content higher than 5C) was observed. Classification of the FISH results by a linear discriminant analysis revealed that 67.6% of the cases were classified in agreement with the CIN classification. These data suggest that chromosome 1 may be considered as a marker chromosome for pre-malignant cervical lesions and that the DNA content measurements are complementary to the FISH results.</abstract><cop>Netherlands</cop><pmid>7713754</pmid><doi>10.1007/bf00164169</doi><tpages>11</tpages></addata></record> |
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| ispartof | The Histochemical journal, 1995-01, Vol.27 (1), p.24-34 |
| issn | 0018-2214 1573-6865 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Adult Aneuploidy Cervical Intraepithelial Neoplasia - genetics Cervical Intraepithelial Neoplasia - pathology Chromosome Aberrations Chromosomes, Human, Pair 1 Densitometry - instrumentation DNA, Neoplasm False Negative Reactions False Positive Reactions Female Humans In Situ Hybridization, Fluorescence Lymphocytes - cytology Neoplasm Staging Uterine Cervical Dysplasia - genetics Uterine Cervical Dysplasia - pathology Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology Vaginal Smears |
| title | Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions |
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