Distribution of colloidal gold tracer within rat parasternal lymph nodes after intrapleural injection
Background: Tracer studies are a important tool to obtain information about the processes involved in the immunological response. Colloidal gold is widely used as a tracer, but its small size of label can cause some difficulty during low‐resolution analysis. To overcome this difficulty, se developed...
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Veröffentlicht in: | The Anatomical record 1995-02, Vol.241 (2), p.175-180 |
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creator | Glazyrin, A. L. Kolesnikov, S. I. Dragun, G. N. Zelentsov, E. L. Zolotarev, K. V. Zorin, Yu A Kulipanov, G. N. Gorchakov, V. N. Dolbnya, I. P. |
description | Background: Tracer studies are a important tool to obtain information about the processes involved in the immunological response. Colloidal gold is widely used as a tracer, but its small size of label can cause some difficulty during low‐resolution analysis. To overcome this difficulty, se developed a new method to follow the route of tracer movement within lymph nodes.
Methods: We applied conventional X‐ray analysis, X‐ray flurescence analysis (XFA) subtractional microscop using synchrotron radiation (SR) beam, light mycroscopy, and ultrastructural analysis to study the distribution and quantity of colloidal gold coupled with albumin with rat parasternal lymph node 2, 4, 6, 8, and 10 h after intrapleural injection of the tracer.
Results: At all the time points XFA‐SR revealed that racer formed a circle with a maximum concentration in the node periphery. XFA‐SR measured colloidal gold concentration in the nodes reached its maximum (0.5−0.75 weight%) in 6–8 h. Substractional microscopy revealed superficially located groups of cells filled with colloidal gold tracer. Light microscopy and ultrastructural analysis confirmed that the tracer was concentrated in the reticular cells, situated in the sinuses of the node. Sinusoidal reticular cells concentrated tracer at much higher rates than sinusoidal macrophages. Four hours after injection, gold appeared in the lysosmes of the follicular reticular cells. At the same time point, evidence of antigen presentation was obtained. Antigen presentation proved to be an extremel sentation was obtained. Antigen presentation proved to be an extremely rare event since only one ultrastructural incident was found in 150 analysed grids.
Conclusion: SR is a valuable tool for the analysis of gold tracer passage with in the living organism. © 1995 Wiley‐Liss, Inc. |
doi_str_mv | 10.1002/ar.1092410205 |
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Methods: We applied conventional X‐ray analysis, X‐ray flurescence analysis (XFA) subtractional microscop using synchrotron radiation (SR) beam, light mycroscopy, and ultrastructural analysis to study the distribution and quantity of colloidal gold coupled with albumin with rat parasternal lymph node 2, 4, 6, 8, and 10 h after intrapleural injection of the tracer.
Results: At all the time points XFA‐SR revealed that racer formed a circle with a maximum concentration in the node periphery. XFA‐SR measured colloidal gold concentration in the nodes reached its maximum (0.5−0.75 weight%) in 6–8 h. Substractional microscopy revealed superficially located groups of cells filled with colloidal gold tracer. Light microscopy and ultrastructural analysis confirmed that the tracer was concentrated in the reticular cells, situated in the sinuses of the node. Sinusoidal reticular cells concentrated tracer at much higher rates than sinusoidal macrophages. Four hours after injection, gold appeared in the lysosmes of the follicular reticular cells. At the same time point, evidence of antigen presentation was obtained. Antigen presentation proved to be an extremel sentation was obtained. Antigen presentation proved to be an extremely rare event since only one ultrastructural incident was found in 150 analysed grids.
Conclusion: SR is a valuable tool for the analysis of gold tracer passage with in the living organism. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 0003-276X</identifier><identifier>EISSN: 1097-0185</identifier><identifier>DOI: 10.1002/ar.1092410205</identifier><identifier>PMID: 7710134</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Colloidal gold ; Colloids ; Gold ; Injections ; Lymph node ; Lymph Nodes - cytology ; Lymph Nodes - diagnostic imaging ; Lymph Nodes - metabolism ; Pleura ; Radiography ; Rats ; Rats, Wistar ; Serum Albumin - metabolism ; Spectrometry, X-Ray Emission ; Subtraction Technique ; Synchrotrons ; X‐ray</subject><ispartof>The Anatomical record, 1995-02, Vol.241 (2), p.175-180</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3365-495633a29f088bdec39c4aa91d6b872b14c009da471ea007adc5a114cd74e8103</citedby><cites>FETCH-LOGICAL-c3365-495633a29f088bdec39c4aa91d6b872b14c009da471ea007adc5a114cd74e8103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Far.1092410205$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Far.1092410205$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7710134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Glazyrin, A. L.</creatorcontrib><creatorcontrib>Kolesnikov, S. I.</creatorcontrib><creatorcontrib>Dragun, G. N.</creatorcontrib><creatorcontrib>Zelentsov, E. L.</creatorcontrib><creatorcontrib>Zolotarev, K. V.</creatorcontrib><creatorcontrib>Zorin, Yu A</creatorcontrib><creatorcontrib>Kulipanov, G. N.</creatorcontrib><creatorcontrib>Gorchakov, V. N.</creatorcontrib><creatorcontrib>Dolbnya, I. P.</creatorcontrib><title>Distribution of colloidal gold tracer within rat parasternal lymph nodes after intrapleural injection</title><title>The Anatomical record</title><addtitle>Anat Rec</addtitle><description>Background: Tracer studies are a important tool to obtain information about the processes involved in the immunological response. Colloidal gold is widely used as a tracer, but its small size of label can cause some difficulty during low‐resolution analysis. To overcome this difficulty, se developed a new method to follow the route of tracer movement within lymph nodes.
Methods: We applied conventional X‐ray analysis, X‐ray flurescence analysis (XFA) subtractional microscop using synchrotron radiation (SR) beam, light mycroscopy, and ultrastructural analysis to study the distribution and quantity of colloidal gold coupled with albumin with rat parasternal lymph node 2, 4, 6, 8, and 10 h after intrapleural injection of the tracer.
Results: At all the time points XFA‐SR revealed that racer formed a circle with a maximum concentration in the node periphery. XFA‐SR measured colloidal gold concentration in the nodes reached its maximum (0.5−0.75 weight%) in 6–8 h. Substractional microscopy revealed superficially located groups of cells filled with colloidal gold tracer. Light microscopy and ultrastructural analysis confirmed that the tracer was concentrated in the reticular cells, situated in the sinuses of the node. Sinusoidal reticular cells concentrated tracer at much higher rates than sinusoidal macrophages. Four hours after injection, gold appeared in the lysosmes of the follicular reticular cells. At the same time point, evidence of antigen presentation was obtained. Antigen presentation proved to be an extremel sentation was obtained. Antigen presentation proved to be an extremely rare event since only one ultrastructural incident was found in 150 analysed grids.
Conclusion: SR is a valuable tool for the analysis of gold tracer passage with in the living organism. © 1995 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Colloidal gold</subject><subject>Colloids</subject><subject>Gold</subject><subject>Injections</subject><subject>Lymph node</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - diagnostic imaging</subject><subject>Lymph Nodes - metabolism</subject><subject>Pleura</subject><subject>Radiography</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serum Albumin - metabolism</subject><subject>Spectrometry, X-Ray Emission</subject><subject>Subtraction Technique</subject><subject>Synchrotrons</subject><subject>X‐ray</subject><issn>0003-276X</issn><issn>1097-0185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVaPHoWcvK1O9iPZPUr9hIIgCt6W2SRrI-lmTXYp_femtKgnT0PmfeaBvIScM7hiAOk1-jirNGeQQnFApvEhEmBlcUimAJAlqeDvx-QkhE8AxnLOJ2QiBAOW5VOib00YvGnGwbiOupZKZ60zCi39cFbRwaPUnq7NsDQd9TjQHj2GQfsuInaz6pe0c0oHim1cUtPFi97q0cfYdJ9absWn5KhFG_TZfs7I2_3d6_wxWTw_PM1vFonMMl4keVXwLMO0aqEsG6VlVskcsWKKN6VIG5ZLgEphLphGAIFKFhi_JJXIdckgm5HLnbf37mvUYahXJkhtLXbajaEWImXAOY9gsgOldyF43da9Nyv0m5pBva21Rl__1hr5i714bFZa_dD7HmMudvnaWL35X1bfvPwxfwMRxoQ-</recordid><startdate>199502</startdate><enddate>199502</enddate><creator>Glazyrin, A. L.</creator><creator>Kolesnikov, S. I.</creator><creator>Dragun, G. N.</creator><creator>Zelentsov, E. L.</creator><creator>Zolotarev, K. V.</creator><creator>Zorin, Yu A</creator><creator>Kulipanov, G. N.</creator><creator>Gorchakov, V. N.</creator><creator>Dolbnya, I. P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199502</creationdate><title>Distribution of colloidal gold tracer within rat parasternal lymph nodes after intrapleural injection</title><author>Glazyrin, A. L. ; Kolesnikov, S. I. ; Dragun, G. N. ; Zelentsov, E. L. ; Zolotarev, K. V. ; Zorin, Yu A ; Kulipanov, G. N. ; Gorchakov, V. N. ; Dolbnya, I. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3365-495633a29f088bdec39c4aa91d6b872b14c009da471ea007adc5a114cd74e8103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Colloidal gold</topic><topic>Colloids</topic><topic>Gold</topic><topic>Injections</topic><topic>Lymph node</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - diagnostic imaging</topic><topic>Lymph Nodes - metabolism</topic><topic>Pleura</topic><topic>Radiography</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serum Albumin - metabolism</topic><topic>Spectrometry, X-Ray Emission</topic><topic>Subtraction Technique</topic><topic>Synchrotrons</topic><topic>X‐ray</topic><toplevel>online_resources</toplevel><creatorcontrib>Glazyrin, A. L.</creatorcontrib><creatorcontrib>Kolesnikov, S. I.</creatorcontrib><creatorcontrib>Dragun, G. N.</creatorcontrib><creatorcontrib>Zelentsov, E. L.</creatorcontrib><creatorcontrib>Zolotarev, K. V.</creatorcontrib><creatorcontrib>Zorin, Yu A</creatorcontrib><creatorcontrib>Kulipanov, G. N.</creatorcontrib><creatorcontrib>Gorchakov, V. N.</creatorcontrib><creatorcontrib>Dolbnya, I. P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Anatomical record</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Glazyrin, A. L.</au><au>Kolesnikov, S. I.</au><au>Dragun, G. N.</au><au>Zelentsov, E. L.</au><au>Zolotarev, K. V.</au><au>Zorin, Yu A</au><au>Kulipanov, G. N.</au><au>Gorchakov, V. N.</au><au>Dolbnya, I. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of colloidal gold tracer within rat parasternal lymph nodes after intrapleural injection</atitle><jtitle>The Anatomical record</jtitle><addtitle>Anat Rec</addtitle><date>1995-02</date><risdate>1995</risdate><volume>241</volume><issue>2</issue><spage>175</spage><epage>180</epage><pages>175-180</pages><issn>0003-276X</issn><eissn>1097-0185</eissn><abstract>Background: Tracer studies are a important tool to obtain information about the processes involved in the immunological response. Colloidal gold is widely used as a tracer, but its small size of label can cause some difficulty during low‐resolution analysis. To overcome this difficulty, se developed a new method to follow the route of tracer movement within lymph nodes.
Methods: We applied conventional X‐ray analysis, X‐ray flurescence analysis (XFA) subtractional microscop using synchrotron radiation (SR) beam, light mycroscopy, and ultrastructural analysis to study the distribution and quantity of colloidal gold coupled with albumin with rat parasternal lymph node 2, 4, 6, 8, and 10 h after intrapleural injection of the tracer.
Results: At all the time points XFA‐SR revealed that racer formed a circle with a maximum concentration in the node periphery. XFA‐SR measured colloidal gold concentration in the nodes reached its maximum (0.5−0.75 weight%) in 6–8 h. Substractional microscopy revealed superficially located groups of cells filled with colloidal gold tracer. Light microscopy and ultrastructural analysis confirmed that the tracer was concentrated in the reticular cells, situated in the sinuses of the node. Sinusoidal reticular cells concentrated tracer at much higher rates than sinusoidal macrophages. Four hours after injection, gold appeared in the lysosmes of the follicular reticular cells. At the same time point, evidence of antigen presentation was obtained. Antigen presentation proved to be an extremel sentation was obtained. Antigen presentation proved to be an extremely rare event since only one ultrastructural incident was found in 150 analysed grids.
Conclusion: SR is a valuable tool for the analysis of gold tracer passage with in the living organism. © 1995 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7710134</pmid><doi>10.1002/ar.1092410205</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Colloidal gold Colloids Gold Injections Lymph node Lymph Nodes - cytology Lymph Nodes - diagnostic imaging Lymph Nodes - metabolism Pleura Radiography Rats Rats, Wistar Serum Albumin - metabolism Spectrometry, X-Ray Emission Subtraction Technique Synchrotrons X‐ray |
title | Distribution of colloidal gold tracer within rat parasternal lymph nodes after intrapleural injection |
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