Thymic expression of the golli-myelin basic protein gene in the SJL/J mouse
The T cell antigen-specific repertoire is thought to be shaped by thymic expression of self molecules. Since a myelin basic protein (MBP)-like gene (golli-MBP) has been reported to be expressed by cells of the immune system, the present study was undertaken to determine whether the golli-MBP gene wa...
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| Veröffentlicht in: | Journal of neuroimmunology 1995-03, Vol.57 (1), p.93-99 |
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| creator | Fritz, Robert B. Kalvakolanu, Indira |
| description | The T cell antigen-specific repertoire is thought to be shaped by thymic expression of self molecules. Since a myelin basic protein (MBP)-like gene (golli-MBP) has been reported to be expressed by cells of the immune system, the present study was undertaken to determine whether the golli-MBP gene was expressed in the mouse thymus and, if so, to characterize transcripts of this gene in this organ. Using exon-specific primers for MBP and golli-MBP, cDNA from thymus and other tissues was amplified, and the amplified products analyzed by Southern blotting with exon-specific oligonucleotide probes. The amplified products were subcloned, and the inserts characterized by DNA sequencing. The thymic transcripts were found to contain golli-MBP exons 1, 2, 3, 5A, 5B, 5C, 6, 7, 8, and 11. |
| doi_str_mv | 10.1016/0165-5728(94)00167-M |
| format | Article |
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Since a myelin basic protein (MBP)-like gene (golli-MBP) has been reported to be expressed by cells of the immune system, the present study was undertaken to determine whether the golli-MBP gene was expressed in the mouse thymus and, if so, to characterize transcripts of this gene in this organ. Using exon-specific primers for MBP and golli-MBP, cDNA from thymus and other tissues was amplified, and the amplified products analyzed by Southern blotting with exon-specific oligonucleotide probes. The amplified products were subcloned, and the inserts characterized by DNA sequencing. The thymic transcripts were found to contain golli-MBP exons 1, 2, 3, 5A, 5B, 5C, 6, 7, 8, and 11.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/0165-5728(94)00167-M</identifier><identifier>PMID: 7535793</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; DNA, Complementary - analysis ; Exons ; Female ; Gene Expression ; Golli-myelin basic protein gene ; Mice ; Molecular Sequence Data ; Mouse ; Myelin Basic Protein - genetics ; RNA, Messenger - analysis ; Thymic expression ; Thymus Gland - metabolism</subject><ispartof>Journal of neuroimmunology, 1995-03, Vol.57 (1), p.93-99</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-66524d6cb367281a0299fe3d88fadd4851e10e8ee2faac7d73871ac7db9f40e63</citedby><cites>FETCH-LOGICAL-c357t-66524d6cb367281a0299fe3d88fadd4851e10e8ee2faac7d73871ac7db9f40e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-5728(94)00167-M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7535793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fritz, Robert B.</creatorcontrib><creatorcontrib>Kalvakolanu, Indira</creatorcontrib><title>Thymic expression of the golli-myelin basic protein gene in the SJL/J mouse</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>The T cell antigen-specific repertoire is thought to be shaped by thymic expression of self molecules. Since a myelin basic protein (MBP)-like gene (golli-MBP) has been reported to be expressed by cells of the immune system, the present study was undertaken to determine whether the golli-MBP gene was expressed in the mouse thymus and, if so, to characterize transcripts of this gene in this organ. Using exon-specific primers for MBP and golli-MBP, cDNA from thymus and other tissues was amplified, and the amplified products analyzed by Southern blotting with exon-specific oligonucleotide probes. The amplified products were subcloned, and the inserts characterized by DNA sequencing. The thymic transcripts were found to contain golli-MBP exons 1, 2, 3, 5A, 5B, 5C, 6, 7, 8, and 11.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>DNA, Complementary - analysis</subject><subject>Exons</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Golli-myelin basic protein gene</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mouse</subject><subject>Myelin Basic Protein - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Thymic expression</subject><subject>Thymus Gland - metabolism</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwzAMhiMEGmPwD0DqCcGhLGnSJr0goYmvsYkD4xylibsF9WMkHWL_npRNHDlEjuXH9usXoXOCbwgm2Ti8NE55Iq5ydo1DxuP5ARoSwZNYsIQcouEfcoxOvP8IUEpZPkADntKU53SIXharbW11BN9rB97btonaMupWEC3bqrJxvYXKNlGhfIDWru0gZEtoIAqxx96ms_E0qtuNh1N0VKrKw9k-jtD7w_1i8hTPXh-fJ3ezWIelXZxlacJMpguaBWVE4STPS6BGiFIZw0RKgGAQAEmplOaGU8FJ_ynykmHI6Ahd7uYGPZ8b8J2srddQVaqBoENynmCaMRpAtgO1a713UMq1s7VyW0mw7D2UvUGyN0jmTP56KOeh7WI_f1PUYP6a9qaF-u2uDuHILwtOem2h0WCsA91J09r_F_wAyvKAMg</recordid><startdate>19950301</startdate><enddate>19950301</enddate><creator>Fritz, Robert B.</creator><creator>Kalvakolanu, Indira</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950301</creationdate><title>Thymic expression of the golli-myelin basic protein gene in the SJL/J mouse</title><author>Fritz, Robert B. ; Kalvakolanu, Indira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-66524d6cb367281a0299fe3d88fadd4851e10e8ee2faac7d73871ac7db9f40e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>DNA, Complementary - analysis</topic><topic>Exons</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Golli-myelin basic protein gene</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Mouse</topic><topic>Myelin Basic Protein - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Thymic expression</topic><topic>Thymus Gland - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fritz, Robert B.</creatorcontrib><creatorcontrib>Kalvakolanu, Indira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fritz, Robert B.</au><au>Kalvakolanu, Indira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thymic expression of the golli-myelin basic protein gene in the SJL/J mouse</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1995-03-01</date><risdate>1995</risdate><volume>57</volume><issue>1</issue><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>The T cell antigen-specific repertoire is thought to be shaped by thymic expression of self molecules. Since a myelin basic protein (MBP)-like gene (golli-MBP) has been reported to be expressed by cells of the immune system, the present study was undertaken to determine whether the golli-MBP gene was expressed in the mouse thymus and, if so, to characterize transcripts of this gene in this organ. Using exon-specific primers for MBP and golli-MBP, cDNA from thymus and other tissues was amplified, and the amplified products analyzed by Southern blotting with exon-specific oligonucleotide probes. The amplified products were subcloned, and the inserts characterized by DNA sequencing. The thymic transcripts were found to contain golli-MBP exons 1, 2, 3, 5A, 5B, 5C, 6, 7, 8, and 11.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7535793</pmid><doi>10.1016/0165-5728(94)00167-M</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of neuroimmunology, 1995-03, Vol.57 (1), p.93-99 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Base Sequence DNA, Complementary - analysis Exons Female Gene Expression Golli-myelin basic protein gene Mice Molecular Sequence Data Mouse Myelin Basic Protein - genetics RNA, Messenger - analysis Thymic expression Thymus Gland - metabolism |
| title | Thymic expression of the golli-myelin basic protein gene in the SJL/J mouse |
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