Biochemical analysis of MEK activation in NIH3T3 fibroblasts. Identification of B-Raf and other activators

Numerous potential activators of MEK have been identified, including c-Raf-1, B-Raf, c-Mos, and a family of MEK kinases. However, little information gives insight into the activators actually utilized in vivo. To address this, we have used column chromatography and a coupled MEK activation assay to...

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