Production of fructose and fructose-3-phosphate in maturing rat lenses
A large increase in glycation of crystallins between 1 and 8 months has been demonstrated in lenses obtained from aging rats. The objective of this study was to investigate if an age-associated increase in the levels of any of the phosphorylated and nonphosphorylated sugars in the aging rat lenses c...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1995-04, Vol.36 (5), p.969-973 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Lal, S Szwergold, BS Taylor, AH Randall, WC Kappler, F Brown, TR |
| description | A large increase in glycation of crystallins between 1 and 8 months has been demonstrated in lenses obtained from aging rats. The objective of this study was to investigate if an age-associated increase in the levels of any of the phosphorylated and nonphosphorylated sugars in the aging rat lenses could be correlated with this increase.
Lenses were obtained from Sprague-Dawley rats ranging in age from 2 to 20 months. Trichloroacetic extracts of these tissues were analyzed by using 31P-NMR for sugar phosphates and high-pressure liquid chromatography equipped with an electrochemical detector for sugars and polyols.
Although no elevation in the lenticular glucose levels was observed, an age-associated increase in the concentrations of polyol pathway-associated metabolites--sorbitol, fructose, sorbitol-3-phosphate, and fructose-3-phosphate--was detected. In contrast, no significant changes were observed in glycolytic or pentose shunt metabolites.
Aging lenses accumulate increased concentrations of fructose and fructose-3-phosphate. Because fructose-3-phosphate is a potent glycating agent and a potential in vivo source of 3-deoxyglucosone, its accumulation in the lens, along with fructose, may be a contributing factor in the age-associated increase of nonenzymatic glycation in rat lenses. |
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Lenses were obtained from Sprague-Dawley rats ranging in age from 2 to 20 months. Trichloroacetic extracts of these tissues were analyzed by using 31P-NMR for sugar phosphates and high-pressure liquid chromatography equipped with an electrochemical detector for sugars and polyols.
Although no elevation in the lenticular glucose levels was observed, an age-associated increase in the concentrations of polyol pathway-associated metabolites--sorbitol, fructose, sorbitol-3-phosphate, and fructose-3-phosphate--was detected. In contrast, no significant changes were observed in glycolytic or pentose shunt metabolites.
Aging lenses accumulate increased concentrations of fructose and fructose-3-phosphate. Because fructose-3-phosphate is a potent glycating agent and a potential in vivo source of 3-deoxyglucosone, its accumulation in the lens, along with fructose, may be a contributing factor in the age-associated increase of nonenzymatic glycation in rat lenses.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 7706047</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Aging ; Animals ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Eye and associated structures. Visual pathways and centers. Vision ; Fructose - analysis ; Fructose - biosynthesis ; Fructosephosphates - analysis ; Fructosephosphates - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Lens, Crystalline - metabolism ; Magnetic Resonance Spectroscopy ; Phosphates - analysis ; Phosphorus Isotopes ; Rats ; Rats, Sprague-Dawley ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 1995-04, Vol.36 (5), p.969-973</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3533944$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7706047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lal, S</creatorcontrib><creatorcontrib>Szwergold, BS</creatorcontrib><creatorcontrib>Taylor, AH</creatorcontrib><creatorcontrib>Randall, WC</creatorcontrib><creatorcontrib>Kappler, F</creatorcontrib><creatorcontrib>Brown, TR</creatorcontrib><title>Production of fructose and fructose-3-phosphate in maturing rat lenses</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>A large increase in glycation of crystallins between 1 and 8 months has been demonstrated in lenses obtained from aging rats. The objective of this study was to investigate if an age-associated increase in the levels of any of the phosphorylated and nonphosphorylated sugars in the aging rat lenses could be correlated with this increase.
Lenses were obtained from Sprague-Dawley rats ranging in age from 2 to 20 months. Trichloroacetic extracts of these tissues were analyzed by using 31P-NMR for sugar phosphates and high-pressure liquid chromatography equipped with an electrochemical detector for sugars and polyols.
Although no elevation in the lenticular glucose levels was observed, an age-associated increase in the concentrations of polyol pathway-associated metabolites--sorbitol, fructose, sorbitol-3-phosphate, and fructose-3-phosphate--was detected. In contrast, no significant changes were observed in glycolytic or pentose shunt metabolites.
Aging lenses accumulate increased concentrations of fructose and fructose-3-phosphate. Because fructose-3-phosphate is a potent glycating agent and a potential in vivo source of 3-deoxyglucosone, its accumulation in the lens, along with fructose, may be a contributing factor in the age-associated increase of nonenzymatic glycation in rat lenses.</description><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fructose - analysis</subject><subject>Fructose - biosynthesis</subject><subject>Fructosephosphates - analysis</subject><subject>Fructosephosphates - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lens, Crystalline - metabolism</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Phosphates - analysis</subject><subject>Phosphorus Isotopes</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j0tLxEAQhAdR1nX1Jwg5qLfAPDOZoyy-YEEPeh46k85mJC9nEhb_vZENe6ou6qOaOiNrphRPlc7FOVlTJrOUSiovyVWM35RyxjhdkZXWNKNSr8nzR-jLyY2-75K-Sqow333EBLryZFKRDnUfhxpGTHyXtDBOwXf7JMCYNNhFjNfkooIm4s2iG_L1_PS5fU137y9v28ddWvNMjakU1LkcnDC6AECmTMGLEp3SkCuhmaSVE4oJztGYMq9Q0dJJNI5VWlEtxYY8HHuH0P9MGEfb-uiwaaDDfopWa06pYdkM3i7gVLRY2iH4FsKvXYbP-d2SQ3TQVAE65-MJE0oII___3R-x2u_rgw9oYwtNM5cyezgcRGaVNZkRf-LQbv4</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Lal, S</creator><creator>Szwergold, BS</creator><creator>Taylor, AH</creator><creator>Randall, WC</creator><creator>Kappler, F</creator><creator>Brown, TR</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Production of fructose and fructose-3-phosphate in maturing rat lenses</title><author>Lal, S ; Szwergold, BS ; Taylor, AH ; Randall, WC ; Kappler, F ; Brown, TR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-430cc8ac397baae159b2bdec57a8537140fc351322e99d8fe50dc4e9c1f750743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fructose - analysis</topic><topic>Fructose - biosynthesis</topic><topic>Fructosephosphates - analysis</topic><topic>Fructosephosphates - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lens, Crystalline - metabolism</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Phosphates - analysis</topic><topic>Phosphorus Isotopes</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lal, S</creatorcontrib><creatorcontrib>Szwergold, BS</creatorcontrib><creatorcontrib>Taylor, AH</creatorcontrib><creatorcontrib>Randall, WC</creatorcontrib><creatorcontrib>Kappler, F</creatorcontrib><creatorcontrib>Brown, TR</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lal, S</au><au>Szwergold, BS</au><au>Taylor, AH</au><au>Randall, WC</au><au>Kappler, F</au><au>Brown, TR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of fructose and fructose-3-phosphate in maturing rat lenses</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>36</volume><issue>5</issue><spage>969</spage><epage>973</epage><pages>969-973</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>A large increase in glycation of crystallins between 1 and 8 months has been demonstrated in lenses obtained from aging rats. The objective of this study was to investigate if an age-associated increase in the levels of any of the phosphorylated and nonphosphorylated sugars in the aging rat lenses could be correlated with this increase.
Lenses were obtained from Sprague-Dawley rats ranging in age from 2 to 20 months. Trichloroacetic extracts of these tissues were analyzed by using 31P-NMR for sugar phosphates and high-pressure liquid chromatography equipped with an electrochemical detector for sugars and polyols.
Although no elevation in the lenticular glucose levels was observed, an age-associated increase in the concentrations of polyol pathway-associated metabolites--sorbitol, fructose, sorbitol-3-phosphate, and fructose-3-phosphate--was detected. In contrast, no significant changes were observed in glycolytic or pentose shunt metabolites.
Aging lenses accumulate increased concentrations of fructose and fructose-3-phosphate. Because fructose-3-phosphate is a potent glycating agent and a potential in vivo source of 3-deoxyglucosone, its accumulation in the lens, along with fructose, may be a contributing factor in the age-associated increase of nonenzymatic glycation in rat lenses.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>7706047</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Investigative ophthalmology & visual science, 1995-04, Vol.36 (5), p.969-973 |
| issn | 0146-0404 1552-5783 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Aging Animals Biological and medical sciences Chromatography, High Pressure Liquid Eye and associated structures. Visual pathways and centers. Vision Fructose - analysis Fructose - biosynthesis Fructosephosphates - analysis Fructosephosphates - biosynthesis Fundamental and applied biological sciences. Psychology Lens, Crystalline - metabolism Magnetic Resonance Spectroscopy Phosphates - analysis Phosphorus Isotopes Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
| title | Production of fructose and fructose-3-phosphate in maturing rat lenses |
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