Fluid Shear Stress Modulates Surface Expression of Adhesion Molecules by Endothelial Cells
We investigated the effect of hemodynamic shear forces on the expression of adhesive molecules, E-selectin, and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC) exposed to laminar (8 dynes/ cm2) or turbulent shear stress (8.6 dynes/cm2 average), or to a st...
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| Veröffentlicht in: | Blood 1995-04, Vol.85 (7), p.1696-1703 |
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| creator | Morigi, Marina Zoja, Carla Figliuzzi, Marina Foppolo, Marco Micheletti, Gianluca Bontempelli, Mario Saronni, Marco Remuzzi, Giuseppe Remuzzi, Andrea |
| description | We investigated the effect of hemodynamic shear forces on the expression of adhesive molecules, E-selectin, and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC) exposed to laminar (8 dynes/ cm2) or turbulent shear stress (8.6 dynes/cm2 average), or to a static condition. Laminar flow induced a significant time dependent increase in the surface expression of ICAM-1, as documented by flow cytometry studies. Endothelial cell surface expression of ICAM-1 in supernatants of HUVEC exposed to laminar flow was not modified, excluding the possibility that HUVEC exposed to laminar flow synthetize factors that upregulate ICAM-1. The effect of laminar flow was specific for ICAM-1, while E-selectin expression was not modulated by the flow condition. Turbulent flow did not affect surface expression of either E-selectin or ICAM-1. To evaluate the functional significance of the laminar-flow-induced increase in ICAM-1 expression, we studied the dynamic interaction of total leukocyte suspension with HUVEC exposed to laminar flow (8 dynes/cm2 for 6 hours) in a parallel plate flow chamber or to static condition. Leukocyte adhesion to HUVEC pre-exposed to flow was significantly enhanced, compared with HUVEC maintained in static condition (233 B1 67 v 43 B1 16 leukocytes/mm2, respectively), and comparable with that of interleukin-1 β treated HUVEC. Mouse monoclonal antibody anti-ICAM-1 completely blocked flow-induced upregulation of leukocyte adhesion. Interleukin-1 β , which upregulated E-selectin expression, caused leukocyte rolling on HUVEC that was significantly lower on flow-conditioned HUVEC and almost absent on untreated static endothelial cells. Thus, laminar flow directly and selectively upregulates ICAM-1 expression on the surface of endothelial cells and promotes leukocyte adhesion. These data are relevant to the current understanding of basic mechanisms that govern local inflammatory reactions and tissue injury. |
| doi_str_mv | 10.1182/blood.V85.7.1696.bloodjournal8571696 |
| format | Article |
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Laminar flow induced a significant time dependent increase in the surface expression of ICAM-1, as documented by flow cytometry studies. Endothelial cell surface expression of ICAM-1 in supernatants of HUVEC exposed to laminar flow was not modified, excluding the possibility that HUVEC exposed to laminar flow synthetize factors that upregulate ICAM-1. The effect of laminar flow was specific for ICAM-1, while E-selectin expression was not modulated by the flow condition. Turbulent flow did not affect surface expression of either E-selectin or ICAM-1. To evaluate the functional significance of the laminar-flow-induced increase in ICAM-1 expression, we studied the dynamic interaction of total leukocyte suspension with HUVEC exposed to laminar flow (8 dynes/cm2 for 6 hours) in a parallel plate flow chamber or to static condition. Leukocyte adhesion to HUVEC pre-exposed to flow was significantly enhanced, compared with HUVEC maintained in static condition (233 B1 67 v 43 B1 16 leukocytes/mm2, respectively), and comparable with that of interleukin-1 β treated HUVEC. Mouse monoclonal antibody anti-ICAM-1 completely blocked flow-induced upregulation of leukocyte adhesion. Interleukin-1 β , which upregulated E-selectin expression, caused leukocyte rolling on HUVEC that was significantly lower on flow-conditioned HUVEC and almost absent on untreated static endothelial cells. Thus, laminar flow directly and selectively upregulates ICAM-1 expression on the surface of endothelial cells and promotes leukocyte adhesion. These data are relevant to the current understanding of basic mechanisms that govern local inflammatory reactions and tissue injury.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V85.7.1696.bloodjournal8571696</identifier><identifier>PMID: 7535583</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Antibodies, Monoclonal - pharmacology ; Biological and medical sciences ; Cell Adhesion - drug effects ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - genetics ; Cell interactions, adhesion ; Cells, Cultured ; E-Selectin ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; Flow Cytometry ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Humans ; Infant, Newborn ; Intercellular Adhesion Molecule-1 - biosynthesis ; Intercellular Adhesion Molecule-1 - genetics ; Interleukin-1 - pharmacology ; Leukocytes - cytology ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Molecular and cellular biology ; Rheology ; Stress, Mechanical ; Umbilical Veins</subject><ispartof>Blood, 1995-04, Vol.85 (7), p.1696-1703</ispartof><rights>1995 American Society of Hematology</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-6d963addbc7f91bde2934d79916c1493c50b7cfd73a0cee3b7b56850acc3edbe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3507151$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7535583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morigi, Marina</creatorcontrib><creatorcontrib>Zoja, Carla</creatorcontrib><creatorcontrib>Figliuzzi, Marina</creatorcontrib><creatorcontrib>Foppolo, Marco</creatorcontrib><creatorcontrib>Micheletti, Gianluca</creatorcontrib><creatorcontrib>Bontempelli, Mario</creatorcontrib><creatorcontrib>Saronni, Marco</creatorcontrib><creatorcontrib>Remuzzi, Giuseppe</creatorcontrib><creatorcontrib>Remuzzi, Andrea</creatorcontrib><title>Fluid Shear Stress Modulates Surface Expression of Adhesion Molecules by Endothelial Cells</title><title>Blood</title><addtitle>Blood</addtitle><description>We investigated the effect of hemodynamic shear forces on the expression of adhesive molecules, E-selectin, and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC) exposed to laminar (8 dynes/ cm2) or turbulent shear stress (8.6 dynes/cm2 average), or to a static condition. Laminar flow induced a significant time dependent increase in the surface expression of ICAM-1, as documented by flow cytometry studies. Endothelial cell surface expression of ICAM-1 in supernatants of HUVEC exposed to laminar flow was not modified, excluding the possibility that HUVEC exposed to laminar flow synthetize factors that upregulate ICAM-1. The effect of laminar flow was specific for ICAM-1, while E-selectin expression was not modulated by the flow condition. Turbulent flow did not affect surface expression of either E-selectin or ICAM-1. To evaluate the functional significance of the laminar-flow-induced increase in ICAM-1 expression, we studied the dynamic interaction of total leukocyte suspension with HUVEC exposed to laminar flow (8 dynes/cm2 for 6 hours) in a parallel plate flow chamber or to static condition. Leukocyte adhesion to HUVEC pre-exposed to flow was significantly enhanced, compared with HUVEC maintained in static condition (233 B1 67 v 43 B1 16 leukocytes/mm2, respectively), and comparable with that of interleukin-1 β treated HUVEC. Mouse monoclonal antibody anti-ICAM-1 completely blocked flow-induced upregulation of leukocyte adhesion. Interleukin-1 β , which upregulated E-selectin expression, caused leukocyte rolling on HUVEC that was significantly lower on flow-conditioned HUVEC and almost absent on untreated static endothelial cells. Thus, laminar flow directly and selectively upregulates ICAM-1 expression on the surface of endothelial cells and promotes leukocyte adhesion. These data are relevant to the current understanding of basic mechanisms that govern local inflammatory reactions and tissue injury.</description><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell interactions, adhesion</subject><subject>Cells, Cultured</subject><subject>E-Selectin</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Intercellular Adhesion Molecule-1 - biosynthesis</subject><subject>Intercellular Adhesion Molecule-1 - genetics</subject><subject>Interleukin-1 - pharmacology</subject><subject>Leukocytes - cytology</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Rheology</subject><subject>Stress, Mechanical</subject><subject>Umbilical Veins</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkM2OFCEURonRjG3rI5iwMK6sEoqmKJaTtkdNZnTR6sINgcutNBO6aKHKOG9v9U9m5cawgNzv5OPmEPKOs5rzrnnvYkq-_tHJWtW81W19GtynKQ82dlIdZ0_Igsumqxhr2FOyYIy11Uor_py8KOWeMb4SjbwiV0oKKTuxID9v4hQ83e7QZrodM5ZC75Kfoh2x0O2UewtIN38OxySkgaaeXvsdnt53KSJMcQbdA90MPo07jMFGusYYy0vyrLex4KvLvSTfbzbf1p-q268fP6-vbyuQbTNWrdetsN47UL3mzmOjxcorrXkLfKUFSOYU9F4JywBROOVk20lmAQR6h2JJ3p57Dzn9mrCMZh8KzBvYAdNUjFJci2Y-S_LhDEJOpWTszSGHvc0PhjNzVGxORs2s2Chz1Gn-oXiueX35b3J79I8lF6dz_uaS2wI29tkOEMojJiRTXPIZ-3LGcHbzO2A2BQIOgD5khNH4FP5vr79tzqi_</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>Morigi, Marina</creator><creator>Zoja, Carla</creator><creator>Figliuzzi, Marina</creator><creator>Foppolo, Marco</creator><creator>Micheletti, Gianluca</creator><creator>Bontempelli, Mario</creator><creator>Saronni, Marco</creator><creator>Remuzzi, Giuseppe</creator><creator>Remuzzi, Andrea</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950401</creationdate><title>Fluid Shear Stress Modulates Surface Expression of Adhesion Molecules by Endothelial Cells</title><author>Morigi, Marina ; Zoja, Carla ; Figliuzzi, Marina ; Foppolo, Marco ; Micheletti, Gianluca ; Bontempelli, Mario ; Saronni, Marco ; Remuzzi, Giuseppe ; Remuzzi, Andrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-6d963addbc7f91bde2934d79916c1493c50b7cfd73a0cee3b7b56850acc3edbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell interactions, adhesion</topic><topic>Cells, Cultured</topic><topic>E-Selectin</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Intercellular Adhesion Molecule-1 - biosynthesis</topic><topic>Intercellular Adhesion Molecule-1 - genetics</topic><topic>Interleukin-1 - pharmacology</topic><topic>Leukocytes - cytology</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Rheology</topic><topic>Stress, Mechanical</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morigi, Marina</creatorcontrib><creatorcontrib>Zoja, Carla</creatorcontrib><creatorcontrib>Figliuzzi, Marina</creatorcontrib><creatorcontrib>Foppolo, Marco</creatorcontrib><creatorcontrib>Micheletti, Gianluca</creatorcontrib><creatorcontrib>Bontempelli, Mario</creatorcontrib><creatorcontrib>Saronni, Marco</creatorcontrib><creatorcontrib>Remuzzi, Giuseppe</creatorcontrib><creatorcontrib>Remuzzi, Andrea</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morigi, Marina</au><au>Zoja, Carla</au><au>Figliuzzi, Marina</au><au>Foppolo, Marco</au><au>Micheletti, Gianluca</au><au>Bontempelli, Mario</au><au>Saronni, Marco</au><au>Remuzzi, Giuseppe</au><au>Remuzzi, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluid Shear Stress Modulates Surface Expression of Adhesion Molecules by Endothelial Cells</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>85</volume><issue>7</issue><spage>1696</spage><epage>1703</epage><pages>1696-1703</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>We investigated the effect of hemodynamic shear forces on the expression of adhesive molecules, E-selectin, and intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVEC) exposed to laminar (8 dynes/ cm2) or turbulent shear stress (8.6 dynes/cm2 average), or to a static condition. Laminar flow induced a significant time dependent increase in the surface expression of ICAM-1, as documented by flow cytometry studies. Endothelial cell surface expression of ICAM-1 in supernatants of HUVEC exposed to laminar flow was not modified, excluding the possibility that HUVEC exposed to laminar flow synthetize factors that upregulate ICAM-1. The effect of laminar flow was specific for ICAM-1, while E-selectin expression was not modulated by the flow condition. Turbulent flow did not affect surface expression of either E-selectin or ICAM-1. To evaluate the functional significance of the laminar-flow-induced increase in ICAM-1 expression, we studied the dynamic interaction of total leukocyte suspension with HUVEC exposed to laminar flow (8 dynes/cm2 for 6 hours) in a parallel plate flow chamber or to static condition. Leukocyte adhesion to HUVEC pre-exposed to flow was significantly enhanced, compared with HUVEC maintained in static condition (233 B1 67 v 43 B1 16 leukocytes/mm2, respectively), and comparable with that of interleukin-1 β treated HUVEC. Mouse monoclonal antibody anti-ICAM-1 completely blocked flow-induced upregulation of leukocyte adhesion. Interleukin-1 β , which upregulated E-selectin expression, caused leukocyte rolling on HUVEC that was significantly lower on flow-conditioned HUVEC and almost absent on untreated static endothelial cells. Thus, laminar flow directly and selectively upregulates ICAM-1 expression on the surface of endothelial cells and promotes leukocyte adhesion. These data are relevant to the current understanding of basic mechanisms that govern local inflammatory reactions and tissue injury.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>7535583</pmid><doi>10.1182/blood.V85.7.1696.bloodjournal8571696</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies, Monoclonal - pharmacology Biological and medical sciences Cell Adhesion - drug effects Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Cell interactions, adhesion Cells, Cultured E-Selectin Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Flow Cytometry Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Regulation Humans Infant, Newborn Intercellular Adhesion Molecule-1 - biosynthesis Intercellular Adhesion Molecule-1 - genetics Interleukin-1 - pharmacology Leukocytes - cytology Membrane Proteins - biosynthesis Membrane Proteins - genetics Molecular and cellular biology Rheology Stress, Mechanical Umbilical Veins |
| title | Fluid Shear Stress Modulates Surface Expression of Adhesion Molecules by Endothelial Cells |
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