Radioimmunoassay of Placental Alkaline Phosphatase in Ovarian Cancer Sera and Tissues
A specific radioimmunoassay for human placental alkaline phosphatase has been developed using the I-labeled enzyme, highly purified with a fast protein liquid chromatography system and an absorbed rabbit antiserum. The sensitivity of this assay was 0.2 U/L. Serum levels of over 0.2 U/L were found in...
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| Veröffentlicht in: | Obstetrics and gynecology (New York. 1953) 1986-12, Vol.68 (6), p.759-764 |
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| container_issue | 6 |
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| container_title | Obstetrics and gynecology (New York. 1953) |
| container_volume | 68 |
| creator | MANO, HISAO FURUHASHI, YOSHIHITO MORIKAWA, YOSIMITSU HATTORI, SEN-EI GOTO, SETSUKO TOMODA, YUTAKA |
| description | A specific radioimmunoassay for human placental alkaline phosphatase has been developed using the I-labeled enzyme, highly purified with a fast protein liquid chromatography system and an absorbed rabbit antiserum. The sensitivity of this assay was 0.2 U/L. Serum levels of over 0.2 U/L were found in 27% of ovarian cancer patients, and most of these elevated enzyme levels occurred with more advanced stages of the disease. On the other hand, almost all ovarian cancer tissue contained detectable levels of the enzyme. Serous adenocarcinoma, endometrioid adenocarcinoma, and dysgerminoma had particularly large amounts. Placental alkaline phosphatase was more frequently detected in tissue than in the serum of ovarian cancer, and therefore may be a useful target in immunodetection and immunotherapy and in studying the histopathology of ovarian cancer. |
| format | Article |
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The sensitivity of this assay was 0.2 U/L. Serum levels of over 0.2 U/L were found in 27% of ovarian cancer patients, and most of these elevated enzyme levels occurred with more advanced stages of the disease. On the other hand, almost all ovarian cancer tissue contained detectable levels of the enzyme. Serous adenocarcinoma, endometrioid adenocarcinoma, and dysgerminoma had particularly large amounts. Placental alkaline phosphatase was more frequently detected in tissue than in the serum of ovarian cancer, and therefore may be a useful target in immunodetection and immunotherapy and in studying the histopathology of ovarian cancer.</description><identifier>ISSN: 0029-7844</identifier><identifier>EISSN: 1873-233X</identifier><identifier>PMID: 3024085</identifier><identifier>CODEN: OBGNAS</identifier><language>eng</language><publisher>New York, NY: The American College of Obstetricians and Gynecologists</publisher><subject>Adenocarcinoma - enzymology ; Adenocarcinoma, Mucinous - enzymology ; Alkaline Phosphatase - analysis ; Alkaline Phosphatase - blood ; Biological and medical sciences ; Cystadenocarcinoma - enzymology ; Female ; Female genital diseases ; GPI-Linked Proteins ; Gynecology. Andrology. Obstetrics ; Humans ; Isoenzymes - analysis ; Isoenzymes - blood ; Medical sciences ; Ovarian Neoplasms - blood ; Ovarian Neoplasms - enzymology ; Radioimmunoassay ; Smoking ; Tumors</subject><ispartof>Obstetrics and gynecology (New York. 1953), 1986-12, Vol.68 (6), p.759-764</ispartof><rights>1986 The American College of Obstetricians and Gynecologists</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8081934$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3024085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MANO, HISAO</creatorcontrib><creatorcontrib>FURUHASHI, YOSHIHITO</creatorcontrib><creatorcontrib>MORIKAWA, YOSIMITSU</creatorcontrib><creatorcontrib>HATTORI, SEN-EI</creatorcontrib><creatorcontrib>GOTO, SETSUKO</creatorcontrib><creatorcontrib>TOMODA, YUTAKA</creatorcontrib><title>Radioimmunoassay of Placental Alkaline Phosphatase in Ovarian Cancer Sera and Tissues</title><title>Obstetrics and gynecology (New York. 1953)</title><addtitle>Obstet Gynecol</addtitle><description>A specific radioimmunoassay for human placental alkaline phosphatase has been developed using the I-labeled enzyme, highly purified with a fast protein liquid chromatography system and an absorbed rabbit antiserum. The sensitivity of this assay was 0.2 U/L. Serum levels of over 0.2 U/L were found in 27% of ovarian cancer patients, and most of these elevated enzyme levels occurred with more advanced stages of the disease. On the other hand, almost all ovarian cancer tissue contained detectable levels of the enzyme. Serous adenocarcinoma, endometrioid adenocarcinoma, and dysgerminoma had particularly large amounts. Placental alkaline phosphatase was more frequently detected in tissue than in the serum of ovarian cancer, and therefore may be a useful target in immunodetection and immunotherapy and in studying the histopathology of ovarian cancer.</description><subject>Adenocarcinoma - enzymology</subject><subject>Adenocarcinoma, Mucinous - enzymology</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Alkaline Phosphatase - blood</subject><subject>Biological and medical sciences</subject><subject>Cystadenocarcinoma - enzymology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>GPI-Linked Proteins</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - blood</subject><subject>Medical sciences</subject><subject>Ovarian Neoplasms - blood</subject><subject>Ovarian Neoplasms - enzymology</subject><subject>Radioimmunoassay</subject><subject>Smoking</subject><subject>Tumors</subject><issn>0029-7844</issn><issn>1873-233X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtrwzAQhE1pSdO0P6GgQ-nNsJYsSz6G0BcEEtoEejNrSSZq5EcluyH_vi4JPS278zHDzkU0TaRgMWXs8zKaAtA8FjJNr6ObEL4AIMlyNokmDGgKkk-j7Ttq29q6HpoWQ8AjaSuydqhM06Mjc7dHZxtD1rs2dDvsMRhiG7L6QW-xIQtslPHkw3gk2GiysSEMJtxGVxW6YO7OcxZtn582i9d4uXp5W8yXcUcl8FiUkORglOJKKIMq1UqjoFpVZSWTSiLXXNNKl6CyDDQapEKJlDNZViByxWbR48m38-33mNsXtQ3KOIeNaYdQCJHInOcwgvdncChro4vO2xr9sTj3MOoPZx2DQlf58S8b_jEJMslZOmLpCTu0rjc-7N1wML7YGXT9rhjrhYxyiJNcZgkdt_jvxNkvlt94Wg</recordid><startdate>198612</startdate><enddate>198612</enddate><creator>MANO, HISAO</creator><creator>FURUHASHI, YOSHIHITO</creator><creator>MORIKAWA, YOSIMITSU</creator><creator>HATTORI, SEN-EI</creator><creator>GOTO, SETSUKO</creator><creator>TOMODA, YUTAKA</creator><general>The American College of Obstetricians and Gynecologists</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198612</creationdate><title>Radioimmunoassay of Placental Alkaline Phosphatase in Ovarian Cancer Sera and Tissues</title><author>MANO, HISAO ; FURUHASHI, YOSHIHITO ; MORIKAWA, YOSIMITSU ; HATTORI, SEN-EI ; GOTO, SETSUKO ; TOMODA, YUTAKA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2805-7b0190ecc5c7ceac4dcda72dcfbf81f8a5d5d2fdb0c660daea27c74538bf079c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenocarcinoma - enzymology</topic><topic>Adenocarcinoma, Mucinous - enzymology</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Alkaline Phosphatase - blood</topic><topic>Biological and medical sciences</topic><topic>Cystadenocarcinoma - enzymology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>GPI-Linked Proteins</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - blood</topic><topic>Medical sciences</topic><topic>Ovarian Neoplasms - blood</topic><topic>Ovarian Neoplasms - enzymology</topic><topic>Radioimmunoassay</topic><topic>Smoking</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MANO, HISAO</creatorcontrib><creatorcontrib>FURUHASHI, YOSHIHITO</creatorcontrib><creatorcontrib>MORIKAWA, YOSIMITSU</creatorcontrib><creatorcontrib>HATTORI, SEN-EI</creatorcontrib><creatorcontrib>GOTO, SETSUKO</creatorcontrib><creatorcontrib>TOMODA, YUTAKA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MANO, HISAO</au><au>FURUHASHI, YOSHIHITO</au><au>MORIKAWA, YOSIMITSU</au><au>HATTORI, SEN-EI</au><au>GOTO, SETSUKO</au><au>TOMODA, YUTAKA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay of Placental Alkaline Phosphatase in Ovarian Cancer Sera and Tissues</atitle><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle><addtitle>Obstet Gynecol</addtitle><date>1986-12</date><risdate>1986</risdate><volume>68</volume><issue>6</issue><spage>759</spage><epage>764</epage><pages>759-764</pages><issn>0029-7844</issn><eissn>1873-233X</eissn><coden>OBGNAS</coden><abstract>A specific radioimmunoassay for human placental alkaline phosphatase has been developed using the I-labeled enzyme, highly purified with a fast protein liquid chromatography system and an absorbed rabbit antiserum. The sensitivity of this assay was 0.2 U/L. Serum levels of over 0.2 U/L were found in 27% of ovarian cancer patients, and most of these elevated enzyme levels occurred with more advanced stages of the disease. On the other hand, almost all ovarian cancer tissue contained detectable levels of the enzyme. Serous adenocarcinoma, endometrioid adenocarcinoma, and dysgerminoma had particularly large amounts. Placental alkaline phosphatase was more frequently detected in tissue than in the serum of ovarian cancer, and therefore may be a useful target in immunodetection and immunotherapy and in studying the histopathology of ovarian cancer.</abstract><cop>New York, NY</cop><pub>The American College of Obstetricians and Gynecologists</pub><pmid>3024085</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0029-7844 |
| ispartof | Obstetrics and gynecology (New York. 1953), 1986-12, Vol.68 (6), p.759-764 |
| issn | 0029-7844 1873-233X |
| language | eng |
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| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | Adenocarcinoma - enzymology Adenocarcinoma, Mucinous - enzymology Alkaline Phosphatase - analysis Alkaline Phosphatase - blood Biological and medical sciences Cystadenocarcinoma - enzymology Female Female genital diseases GPI-Linked Proteins Gynecology. Andrology. Obstetrics Humans Isoenzymes - analysis Isoenzymes - blood Medical sciences Ovarian Neoplasms - blood Ovarian Neoplasms - enzymology Radioimmunoassay Smoking Tumors |
| title | Radioimmunoassay of Placental Alkaline Phosphatase in Ovarian Cancer Sera and Tissues |
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