Flow Cytometric Analysis of Transmembrane Phospholipid Movement in Bull Sperm

Fluorescent phospholipids are useful to investigate phospholipid dynamics in biological membranes. We used flow cytometry to investigate transbilayer phospholipid movement in live sperm cells. Acyl-labeled N-4-nitrobenzo-2-oxa-1,3-diazole (NBD) -phosphatidylcholine (-PC), -phosphatidylethanolamine (...

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Veröffentlicht in:	Biochemistry (Easton) 1995-03, Vol.34 (12), p.3907-3915
Hauptverfasser:	Nolan, John P, Magargee, Susan F, Posner, Richard G, Hammerstedt, Roy H
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Chloro-7-nitrobenzofurazan Animals Cattle Cell Membrane - metabolism Computer Simulation Dithionite Flow Cytometry - methods In Vitro Techniques Intracellular Membranes - metabolism Kinetics Lipid Bilayers Male Membrane Lipids - metabolism Models, Biological Phospholipids - metabolism Spermatozoa - metabolism Time Factors
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