Polymerase Chain Reaction (PCR) Search for Viral Nucleic Acid Sequences in Schizophrenia
Previous studies looking for evidence of viral infection in schizophrenics have yielded conflicting results. We searched for viral nucleic acids to test the hypothesis of the viral aetiology of schizophrenia. We used the polymerase chain reaction (PCR) to search for cytomegalovirus (CMV), human immu...
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| Veröffentlicht in: | British journal of psychiatry 1995-01, Vol.166 (1), p.55-60 |
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| creator | Sierra-Honigmann, A. M. Carbone, K. M. Yolken, R. H. |
| description | Previous studies looking for evidence of viral infection in schizophrenics have yielded conflicting results. We searched for viral nucleic acids to test the hypothesis of the viral aetiology of schizophrenia.
We used the polymerase chain reaction (PCR) to search for cytomegalovirus (CMV), human immunodeficiency virus (HIV), influenza A, Borna disease virus (BDV), and bovine viral diarrhoea virus (BVDV) in: hippocampus from three schizophrenic and three non-schizophrenic subjects; cerebrospinal fluid (CSF) from 48 schizophrenic patients; CSF and peripheral blood mononuclear cells (PBMC) from nine sets of identical twins discordant for schizophrenia; and SK-N-SHEP cells co-cultured with schizophrenic and non-schizophrenic brain homogenates. All patients met DSM-III-R criteria.
Virus-specific nucleic acids were not found in any of the samples tested.
The absence of viral nucleic acids in the samples tested suggest that, in these patients, schizophrenia is not associated with a persistent or latent infection due to these viruses. |
| doi_str_mv | 10.1192/bjp.166.1.55 |
| format | Article |
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We used the polymerase chain reaction (PCR) to search for cytomegalovirus (CMV), human immunodeficiency virus (HIV), influenza A, Borna disease virus (BDV), and bovine viral diarrhoea virus (BVDV) in: hippocampus from three schizophrenic and three non-schizophrenic subjects; cerebrospinal fluid (CSF) from 48 schizophrenic patients; CSF and peripheral blood mononuclear cells (PBMC) from nine sets of identical twins discordant for schizophrenia; and SK-N-SHEP cells co-cultured with schizophrenic and non-schizophrenic brain homogenates. All patients met DSM-III-R criteria.
Virus-specific nucleic acids were not found in any of the samples tested.
The absence of viral nucleic acids in the samples tested suggest that, in these patients, schizophrenia is not associated with a persistent or latent infection due to these viruses.</description><identifier>ISSN: 0007-1250</identifier><identifier>EISSN: 1472-1465</identifier><identifier>DOI: 10.1192/bjp.166.1.55</identifier><identifier>PMID: 7894877</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Acids ; Adult ; AIDS/HIV ; Blood ; Borna disease ; Borna disease virus - genetics ; Brain ; Cell Line ; Cerebrospinal fluid ; Cerebrospinal Fluid - virology ; Chemical analysis ; Cytomegalovirus ; Cytomegalovirus - genetics ; Diarrhea ; Diarrhea Viruses, Bovine Viral - genetics ; Diseases in Twins - genetics ; DNA, Viral - genetics ; Etiology ; Female ; Gene Library ; Hippocampus ; Hippocampus - pathology ; Hippocampus - virology ; HIV ; HIV - genetics ; Human immunodeficiency virus ; Humans ; Infections ; Influenza ; Influenza A ; Influenza A virus - genetics ; Latent infection ; Leukocytes (mononuclear) ; Male ; Mental disorders ; Middle Aged ; Molecular Sequence Data ; Monocytes - pathology ; Neuroblastoma ; Neurocognitive Disorders - genetics ; Neurocognitive Disorders - virology ; Nucleic acids ; Peripheral blood mononuclear cells ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Regulatory Sequences, Nucleic Acid - genetics ; RNA Probes ; RNA, Viral - genetics ; Schizophrenia ; Schizophrenia - genetics ; Schizophrenia - virology ; Sequences ; Twins ; Twins, Monozygotic - genetics ; Viral infections ; Virus Diseases - genetics ; Virus Diseases - virology ; Viruses</subject><ispartof>British journal of psychiatry, 1995-01, Vol.166 (1), p.55-60</ispartof><rights>Copyright © 1995 The Royal College of Psychiatrists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-eeca4fa0aed837c7bd2d42c588780132ef8592c5ca00bbada2f147c6738381fb3</citedby><cites>FETCH-LOGICAL-c378t-eeca4fa0aed837c7bd2d42c588780132ef8592c5ca00bbada2f147c6738381fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0007125000143296/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,12825,27901,27902,30976,55603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7894877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sierra-Honigmann, A. M.</creatorcontrib><creatorcontrib>Carbone, K. M.</creatorcontrib><creatorcontrib>Yolken, R. H.</creatorcontrib><title>Polymerase Chain Reaction (PCR) Search for Viral Nucleic Acid Sequences in Schizophrenia</title><title>British journal of psychiatry</title><addtitle>Br J Psychiatry</addtitle><description>Previous studies looking for evidence of viral infection in schizophrenics have yielded conflicting results. We searched for viral nucleic acids to test the hypothesis of the viral aetiology of schizophrenia.
We used the polymerase chain reaction (PCR) to search for cytomegalovirus (CMV), human immunodeficiency virus (HIV), influenza A, Borna disease virus (BDV), and bovine viral diarrhoea virus (BVDV) in: hippocampus from three schizophrenic and three non-schizophrenic subjects; cerebrospinal fluid (CSF) from 48 schizophrenic patients; CSF and peripheral blood mononuclear cells (PBMC) from nine sets of identical twins discordant for schizophrenia; and SK-N-SHEP cells co-cultured with schizophrenic and non-schizophrenic brain homogenates. All patients met DSM-III-R criteria.
Virus-specific nucleic acids were not found in any of the samples tested.
The absence of viral nucleic acids in the samples tested suggest that, in these patients, schizophrenia is not associated with a persistent or latent infection due to these viruses.</description><subject>Acids</subject><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Blood</subject><subject>Borna disease</subject><subject>Borna disease virus - genetics</subject><subject>Brain</subject><subject>Cell Line</subject><subject>Cerebrospinal fluid</subject><subject>Cerebrospinal Fluid - virology</subject><subject>Chemical analysis</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - genetics</subject><subject>Diarrhea</subject><subject>Diarrhea Viruses, Bovine Viral - genetics</subject><subject>Diseases in Twins - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Etiology</subject><subject>Female</subject><subject>Gene Library</subject><subject>Hippocampus</subject><subject>Hippocampus - pathology</subject><subject>Hippocampus - virology</subject><subject>HIV</subject><subject>HIV - genetics</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A virus - genetics</subject><subject>Latent infection</subject><subject>Leukocytes (mononuclear)</subject><subject>Male</subject><subject>Mental disorders</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Monocytes - pathology</subject><subject>Neuroblastoma</subject><subject>Neurocognitive Disorders - genetics</subject><subject>Neurocognitive Disorders - virology</subject><subject>Nucleic acids</subject><subject>Peripheral blood mononuclear cells</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Regulatory Sequences, Nucleic Acid - genetics</subject><subject>RNA Probes</subject><subject>RNA, Viral - genetics</subject><subject>Schizophrenia</subject><subject>Schizophrenia - genetics</subject><subject>Schizophrenia - virology</subject><subject>Sequences</subject><subject>Twins</subject><subject>Twins, Monozygotic - genetics</subject><subject>Viral infections</subject><subject>Virus Diseases - genetics</subject><subject>Virus Diseases - virology</subject><subject>Viruses</subject><issn>0007-1250</issn><issn>1472-1465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>7QJ</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkN1LHDEUxUOp6Kp981UIFEoFZ8zHzCT7KEvVgrSitvQtZO7ccbLMV5MdyvrXG9nFivgULveXc849hBxxlnI-F2flckx5UaQ8zfMPZMYzJRKeFflHMmOMqYSLnO2R_RCWcZSZULtkV-l5ppWakT83Q7vu0NuAdNFY19NbtLByQ0-_3ixuT-gdWg8NrQdPfztvW_pjghYd0HNwVdz-nbAHDDT-vIPGPQ5j47F39pDs1LYN-Gn7HpBfF9_uF1fJ9c_L74vz6wSk0qsEEWxWW2ax0lKBKitRZQJyrZVmXAqsdT6PM1jGytJWVtTxQCiU1FLzupQH5MtGd_RDzBJWpnMBsG1tj8MUjFJcq4LPI_j5DbgcJt_HbEZIGc24FjpSpxsK_BCCx9qM3nXWrw1n5rluE-s2sW7DTZ5H_HgrOpUdVi_wtt-4p5t94x6af86j8TCGNTSvJdKto-1K76oH_B_sXc8nccKUfA</recordid><startdate>199501</startdate><enddate>199501</enddate><creator>Sierra-Honigmann, A. 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M.</au><au>Carbone, K. M.</au><au>Yolken, R. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase Chain Reaction (PCR) Search for Viral Nucleic Acid Sequences in Schizophrenia</atitle><jtitle>British journal of psychiatry</jtitle><addtitle>Br J Psychiatry</addtitle><date>1995-01</date><risdate>1995</risdate><volume>166</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0007-1250</issn><eissn>1472-1465</eissn><abstract>Previous studies looking for evidence of viral infection in schizophrenics have yielded conflicting results. We searched for viral nucleic acids to test the hypothesis of the viral aetiology of schizophrenia.
We used the polymerase chain reaction (PCR) to search for cytomegalovirus (CMV), human immunodeficiency virus (HIV), influenza A, Borna disease virus (BDV), and bovine viral diarrhoea virus (BVDV) in: hippocampus from three schizophrenic and three non-schizophrenic subjects; cerebrospinal fluid (CSF) from 48 schizophrenic patients; CSF and peripheral blood mononuclear cells (PBMC) from nine sets of identical twins discordant for schizophrenia; and SK-N-SHEP cells co-cultured with schizophrenic and non-schizophrenic brain homogenates. All patients met DSM-III-R criteria.
Virus-specific nucleic acids were not found in any of the samples tested.
The absence of viral nucleic acids in the samples tested suggest that, in these patients, schizophrenia is not associated with a persistent or latent infection due to these viruses.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>7894877</pmid><doi>10.1192/bjp.166.1.55</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1250 |
| ispartof | British journal of psychiatry, 1995-01, Vol.166 (1), p.55-60 |
| issn | 0007-1250 1472-1465 |
| language | eng |
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| source | Applied Social Sciences Index & Abstracts (ASSIA); MEDLINE; Cambridge Journals |
| subjects | Acids Adult AIDS/HIV Blood Borna disease Borna disease virus - genetics Brain Cell Line Cerebrospinal fluid Cerebrospinal Fluid - virology Chemical analysis Cytomegalovirus Cytomegalovirus - genetics Diarrhea Diarrhea Viruses, Bovine Viral - genetics Diseases in Twins - genetics DNA, Viral - genetics Etiology Female Gene Library Hippocampus Hippocampus - pathology Hippocampus - virology HIV HIV - genetics Human immunodeficiency virus Humans Infections Influenza Influenza A Influenza A virus - genetics Latent infection Leukocytes (mononuclear) Male Mental disorders Middle Aged Molecular Sequence Data Monocytes - pathology Neuroblastoma Neurocognitive Disorders - genetics Neurocognitive Disorders - virology Nucleic acids Peripheral blood mononuclear cells Polymerase chain reaction Polymerase Chain Reaction - methods Regulatory Sequences, Nucleic Acid - genetics RNA Probes RNA, Viral - genetics Schizophrenia Schizophrenia - genetics Schizophrenia - virology Sequences Twins Twins, Monozygotic - genetics Viral infections Virus Diseases - genetics Virus Diseases - virology Viruses |
| title | Polymerase Chain Reaction (PCR) Search for Viral Nucleic Acid Sequences in Schizophrenia |
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