A Method for Probing the Topography and Interactions of Proteins: Footprinting of Myoglobin

We describe a procedure for mapping residues on the surface of a protein molecule to its sequence, using a scheme that is analogous to nucleic acid footprinting. The protein is end labeled radioactively and subjected to limited proteolysis, and the products are analyzed by denaturing polyacrylamide...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1995-03, Vol.92 (6), p.2111-2115
Hauptverfasser:	Zhong, Min, Lin, Laura, Kallenbach, Neville R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Amino Acid Sequence Animals Antibodies Antibodies, Monoclonal Apoproteins - chemistry Binding Sites Biochemistry Cattle Cloning, Molecular Endopeptidases Enzymes Escherichia coli Gels Isotope Labeling - methods Marine Models, Molecular Molecular Sequence Data Monoclonal antibodies Myocardium - metabolism Myoglobin - biosynthesis Myoglobin - chemistry Myoglobin - metabolism Nucleic acids Phosphorus Radioisotopes Physeter Product labeling Protein Kinases - chemistry Protein Kinases - metabolism Protein Structure, Secondary Proteins Proteins - chemistry Reactivity Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - metabolism Spermatozoa Whales Whales & whaling
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