Identification of the NADP(H) binding site of rat liver microsomal 5 alpha-reductase (isozyme-1): purification of a photolabeled peptide corresponding to the adenine binding domain

We have previously shown that [2'-32P]-2-azido-NADP+ is an effective probe of the NADP-(H) binding site of rat liver microsomal 5 alpha-reductase (5 alpha R-1) [Bhattacharyya et al. (1994) Steroids 59, 634-641]. PEG-fractionated (6.5%) detergent-solubilized preparations (40 mg) containing 5 alp...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemistry (Easton) 1995-03, Vol.34 (11), p.3663-3669
Hauptverfasser:	Bhattacharyya, A K, Chavan, A J, Haley, B E, Taylor, M F, Collins, D C
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - metabolism Amino Acid Sequence Animals Binding Sites Cholestenone 5 alpha-Reductase Chromatography, Affinity Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Female Isoenzymes - chemistry Isoenzymes - metabolism Microsomes, Liver - enzymology Molecular Sequence Data NADP - metabolism Oxidoreductases - chemistry Oxidoreductases - metabolism Peptide Fragments - isolation & purification Peptide Fragments - metabolism Photochemistry Rats Rats, Sprague-Dawley
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!