Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants
The viral antigens recognized by cytotoxic T cells (CTL) have not been defined in most viruses infecting mouse or man. Natural or artificial virus recombinants can be used to determine the antigen specificity of CTL directed against viruses with segmented genomes, such as influenza, but this techniq...
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| Veröffentlicht in: | The Journal of immunology (1950) 1986-12, Vol.137 (12), p.3973-3977 |
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| container_end_page | 3977 |
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| container_issue | 12 |
| container_start_page | 3973 |
| container_title | The Journal of immunology (1950) |
| container_volume | 137 |
| creator | Bangham, CR Openshaw, PJ Ball, LA King, AM Wertz, GW Askonas, BA |
| description | The viral antigens recognized by cytotoxic T cells (CTL) have not been defined in most viruses infecting mouse or man. Natural or artificial virus recombinants can be used to determine the antigen specificity of CTL directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. We describe here the use of recombinant vaccinia viruses, containing cDNA corresponding to either the nucleoprotein (N) gene or the major surface glycoprotein (G) gene of human respiratory syncytial virus (RSV), to examine the antigen specificity of anti-RSV cytotoxic T cells from humans and mice. The results demonstrate that the RSV N protein is one of the target antigens for CTL in man and mouse, whereas the G protein was not recognized and can at best represent a minor target antigen for CTL. |
| doi_str_mv | 10.4049/jimmunol.137.12.3973 |
| format | Article |
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Natural or artificial virus recombinants can be used to determine the antigen specificity of CTL directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. We describe here the use of recombinant vaccinia viruses, containing cDNA corresponding to either the nucleoprotein (N) gene or the major surface glycoprotein (G) gene of human respiratory syncytial virus (RSV), to examine the antigen specificity of anti-RSV cytotoxic T cells from humans and mice. The results demonstrate that the RSV N protein is one of the target antigens for CTL in man and mouse, whereas the G protein was not recognized and can at best represent a minor target antigen for CTL.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capsid - immunology</subject><subject>Epitopes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>HLA Antigens - immunology</subject><subject>Humans</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>respiratory syncytial virus</subject><subject>Respiratory Syncytial Viruses - immunology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccinia virus - immunology</subject><subject>Viral Core Proteins - immunology</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Virology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEKtPCG4DkBUJFIoOdOE6yrCpokSrYlLXlODczHvkn2E6n4eF4tjrMULpjZeme755j3ZNlbwheU0zbTztlzGSdXpOyXpNiXbZ1-SxbkarCOWOYPc9WGBdFTmpWv8xOQ9hhjBku6El2UtCS4Iqsst_XkxEWCdsjM3llAck5uujulUS3SILWAYURpBrSIDrkIYzKi-j8jMJsE6yERnfKTyFp0m2s-gUobmGZJcVOUoMbvYugLDr_9uEj6qaIrIt_ICN2zqONnuU_5ioxcD-mpAA96mZ0J6RUVoknMaZTVtgYXmUvBqEDvD6-Z9mPL59vL6_zm-9XXy8vbnJJKYl5X5GKdZQx2vREAmFS9jUZeowbaIksaIv7hlGom552kgkxENoMFeBm6LqyxeVZ9v7gm375c4IQuVFhuY6w4KbA63q5Mq3_CxKauKKsEkgPoPQuBA8DH70yws-cYL70y__2y1O_nBR86TetvT36T52B_nHpWGjS3x11EaTQgxdWqvCINZgmaLE5P2BbtdnulQcejNA6mRK-3--fJj4ACFDClA</recordid><startdate>19861215</startdate><enddate>19861215</enddate><creator>Bangham, CR</creator><creator>Openshaw, PJ</creator><creator>Ball, LA</creator><creator>King, AM</creator><creator>Wertz, GW</creator><creator>Askonas, BA</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19861215</creationdate><title>Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants</title><author>Bangham, CR ; Openshaw, PJ ; Ball, LA ; King, AM ; Wertz, GW ; Askonas, BA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-d5156b46648d1ce16ccd71fd008e91c2490d864e78d4bc6aaf148f5e08fbb3903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capsid - immunology</topic><topic>Epitopes - immunology</topic><topic>Fundamental and applied biological sciences. 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Natural or artificial virus recombinants can be used to determine the antigen specificity of CTL directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. We describe here the use of recombinant vaccinia viruses, containing cDNA corresponding to either the nucleoprotein (N) gene or the major surface glycoprotein (G) gene of human respiratory syncytial virus (RSV), to examine the antigen specificity of anti-RSV cytotoxic T cells from humans and mice. The results demonstrate that the RSV N protein is one of the target antigens for CTL in man and mouse, whereas the G protein was not recognized and can at best represent a minor target antigen for CTL.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>2431051</pmid><doi>10.4049/jimmunol.137.12.3973</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Capsid - immunology Epitopes - immunology Fundamental and applied biological sciences. Psychology Glycoproteins - genetics Glycoproteins - immunology HLA Antigens - immunology Humans Mice Microbiology Recombinant Proteins - genetics Recombinant Proteins - immunology respiratory syncytial virus Respiratory Syncytial Viruses - immunology T-Lymphocytes, Cytotoxic - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccinia virus - immunology Viral Core Proteins - immunology Viral Proteins - genetics Viral Proteins - immunology Virology |
| title | Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants |
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