Increased prevalence of K-ras oncogene mutations in lung adenocarcinoma
Reported estimates of ras mutation prevalence in lung adenocarcinoma of 15-24% may be underestimates because of the insensitivity of the assays used. We have devised a rapid, non-radioactive assay for ras mutations, which detects 1 mutant allele/10(3) normal alleles and have used it to study DNA iso...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1995-04, Vol.55 (7), p.1444-1447 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | MILLS, N. E FISHMAN, C. L ROM, W. N DUBIN, N JACOBSON, D. R |
| description | Reported estimates of ras mutation prevalence in lung adenocarcinoma of 15-24% may be underestimates because of the insensitivity of the assays used. We have devised a rapid, non-radioactive assay for ras mutations, which detects 1 mutant allele/10(3) normal alleles and have used it to study DNA isolated from 53 lung tumor samples (including 28 adenocarcinomas) previously analyzed by PCR/allele specific oligonucleotide hybridization, which is less sensitive. We detected mutations in 13 of 28 samples, including 7 not detected by PCR/allele specific oligonucleotide hybridization. We also found ras mutations in 14 of 25 previously unstudied samples (56%). Our results indicate that the prevalence of K-ras codon 12 mutations in lung adenocarcinoma is higher than previously reported; thus, ras mutations may be more clinically useful as molecular markers for lung cancer than has been appreciated. |
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E ; FISHMAN, C. L ; ROM, W. N ; DUBIN, N ; JACOBSON, D. R</creator><creatorcontrib>MILLS, N. E ; FISHMAN, C. L ; ROM, W. N ; DUBIN, N ; JACOBSON, D. R</creatorcontrib><description>Reported estimates of ras mutation prevalence in lung adenocarcinoma of 15-24% may be underestimates because of the insensitivity of the assays used. We have devised a rapid, non-radioactive assay for ras mutations, which detects 1 mutant allele/10(3) normal alleles and have used it to study DNA isolated from 53 lung tumor samples (including 28 adenocarcinomas) previously analyzed by PCR/allele specific oligonucleotide hybridization, which is less sensitive. We detected mutations in 13 of 28 samples, including 7 not detected by PCR/allele specific oligonucleotide hybridization. We also found ras mutations in 14 of 25 previously unstudied samples (56%). Our results indicate that the prevalence of K-ras codon 12 mutations in lung adenocarcinoma is higher than previously reported; thus, ras mutations may be more clinically useful as molecular markers for lung cancer than has been appreciated.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 7882350</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adenocarcinoma - genetics ; Base Sequence ; Biological and medical sciences ; Codon - genetics ; DNA Mutational Analysis ; DNA, Neoplasm - analysis ; DNA, Neoplasm - genetics ; Genes, ras - genetics ; Humans ; Lung Neoplasms - genetics ; Medical sciences ; Molecular Sequence Data ; Mutation - genetics ; Pneumology ; Sensitivity and Specificity ; Tumors of the respiratory system and mediastinum</subject><ispartof>Cancer research (Chicago, Ill.), 1995-04, Vol.55 (7), p.1444-1447</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3490298$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7882350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MILLS, N. 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We also found ras mutations in 14 of 25 previously unstudied samples (56%). Our results indicate that the prevalence of K-ras codon 12 mutations in lung adenocarcinoma is higher than previously reported; thus, ras mutations may be more clinically useful as molecular markers for lung cancer than has been appreciated.</description><subject>Adenocarcinoma - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Codon - genetics</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>DNA, Neoplasm - genetics</subject><subject>Genes, ras - genetics</subject><subject>Humans</subject><subject>Lung Neoplasms - genetics</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>Pneumology</subject><subject>Sensitivity and Specificity</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j01LxDAYhIMoa139CUIO4q2QNM1Hj7LourjgRc_lbfpmrbRJTVrBf2_B4mkY5mGYOSMZl8LkuizlOckYYyaXpS4uyVVKn4uVnMkN2WhjCiFZRvYHbyNCwpaOEb-hR2-RBkdf8giJBm_DCT3SYZ5g6oJPtPO0n_2JQos-WIi282GAa3LhoE94s-qWvD89vu2e8-Pr_rB7OOYfhTJTXjFWskZbroVuUQpeMcFL2aI2ymIhTKMqaEE4YYVrQBkr1LJUQcWcA6XEltz_9Y4xfM2YpnroksW-B49hTrXWXHNmzALeruDcDNjWY-wGiD_1-nzJ79YckoXeRfC2S_-YKCtWVEb8Ao7HYdQ</recordid><startdate>19950401</startdate><enddate>19950401</enddate><creator>MILLS, N. 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E</creatorcontrib><creatorcontrib>FISHMAN, C. L</creatorcontrib><creatorcontrib>ROM, W. N</creatorcontrib><creatorcontrib>DUBIN, N</creatorcontrib><creatorcontrib>JACOBSON, D. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MILLS, N. E</au><au>FISHMAN, C. L</au><au>ROM, W. N</au><au>DUBIN, N</au><au>JACOBSON, D. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased prevalence of K-ras oncogene mutations in lung adenocarcinoma</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1995-04-01</date><risdate>1995</risdate><volume>55</volume><issue>7</issue><spage>1444</spage><epage>1447</epage><pages>1444-1447</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Reported estimates of ras mutation prevalence in lung adenocarcinoma of 15-24% may be underestimates because of the insensitivity of the assays used. We have devised a rapid, non-radioactive assay for ras mutations, which detects 1 mutant allele/10(3) normal alleles and have used it to study DNA isolated from 53 lung tumor samples (including 28 adenocarcinomas) previously analyzed by PCR/allele specific oligonucleotide hybridization, which is less sensitive. We detected mutations in 13 of 28 samples, including 7 not detected by PCR/allele specific oligonucleotide hybridization. We also found ras mutations in 14 of 25 previously unstudied samples (56%). Our results indicate that the prevalence of K-ras codon 12 mutations in lung adenocarcinoma is higher than previously reported; thus, ras mutations may be more clinically useful as molecular markers for lung cancer than has been appreciated.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>7882350</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1995-04, Vol.55 (7), p.1444-1447 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenocarcinoma - genetics Base Sequence Biological and medical sciences Codon - genetics DNA Mutational Analysis DNA, Neoplasm - analysis DNA, Neoplasm - genetics Genes, ras - genetics Humans Lung Neoplasms - genetics Medical sciences Molecular Sequence Data Mutation - genetics Pneumology Sensitivity and Specificity Tumors of the respiratory system and mediastinum |
| title | Increased prevalence of K-ras oncogene mutations in lung adenocarcinoma |
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