Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid
Iron-binding proteins have antibacterial activity; they have been identified in lung secretions, but their role in pulmonary antibacterial defenses is unclear. Murine lactoferrin and murine transferrin were used to generate polyclonal antiserum to lactoferrin and to transferrin, and the specificity...
Gespeichert in:
| Veröffentlicht in: | The Journal of infectious diseases 1986-12, Vol.154 (6), p.959-965 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 965 |
|---|---|
| container_issue | 6 |
| container_start_page | 959 |
| container_title | The Journal of infectious diseases |
| container_volume | 154 |
| creator | LaForce, F. Marc Boose, Dorothy S. Ellison, Richard T. |
| description | Iron-binding proteins have antibacterial activity; they have been identified in lung secretions, but their role in pulmonary antibacterial defenses is unclear. Murine lactoferrin and murine transferrin were used to generate polyclonal antiserum to lactoferrin and to transferrin, and the specificity of both antisera was shown by western blot. Mice were exposed to either aerosolized Escherichia coli or Staphylococcus aureus; they were killed 1, 4, 24, or 48 hr later; and their lungs were lavaged. Wemeasured the levels of transferrin, lactoferrin, and albumin and did a cell count for the lavage fluid. The predominant ironbinding protein in resting animals wastransferrin. Aerosolized E. coli caused a brisk PMNL response in the lungs that was associated with a major increase in the levels of lactoferrin. Challenge with S. aureus was associated with a moderate increase in the number of macrophages and a moderate decrease in the levels of transferrin and iron but no change in the levels of lactoferrin. The levels of iron-binding protein can vary according to the type of inflammatory response. |
| doi_str_mv | 10.1093/infdis/154.6.959 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_77170191</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30104979</jstor_id><sourcerecordid>30104979</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-d343b0c8ae74e7834d455c909e113677a96326fcbea2835687c9455e1e5c8f7c3</originalsourceid><addsrcrecordid>eNpFkc1vEzEQxS0EKqFw54LkA-K2qb3e9cexVAmtFAESn-JiObOzjcvGTu1doPz1OEpoLzOjeb95kp8JecnZnDMjznzoO5_PeNvM5dy05hGZ8VaoSkouHpMZY3VdcW3MU_Is5xvGWCOkOiEnokAFnJHfi75HGGns6TmmmOPg_2JHFxk2mDxsvKNQdtSFjn4a3W5zN0SIAFOmbkpYWgz0KpWyJ_ZD9daHzodr-jHFEX3I1Ae6mspi5X65a6TLYfLdc_Kkd0PGF8d-Sr4sF58vLqvVh3dXF-erCoQWY9WJRqwZaIeqQaVF0zVtC4YZ5Ly8RDkjRS17WKOrtWilVmAKgRxb0L0CcUreHHx3Kd5OmEe79RlwGFzAOGWrFFeMG15AdgChpJAT9naX_NalO8uZ3WdtD1nbkrWVtmRdTl4dvaf1Frv7g2O4RX991F0GN_TJBSgG_zFda1b-5sHmJo8xPbgwzhqj9np10H0e8c-97tJPK5VQrb38_sN-M_JrU7-v7VL8A-UWoWA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77170191</pqid></control><display><type>article</type><title>Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><creator>LaForce, F. Marc ; Boose, Dorothy S. ; Ellison, Richard T.</creator><creatorcontrib>LaForce, F. Marc ; Boose, Dorothy S. ; Ellison, Richard T.</creatorcontrib><description>Iron-binding proteins have antibacterial activity; they have been identified in lung secretions, but their role in pulmonary antibacterial defenses is unclear. Murine lactoferrin and murine transferrin were used to generate polyclonal antiserum to lactoferrin and to transferrin, and the specificity of both antisera was shown by western blot. Mice were exposed to either aerosolized Escherichia coli or Staphylococcus aureus; they were killed 1, 4, 24, or 48 hr later; and their lungs were lavaged. Wemeasured the levels of transferrin, lactoferrin, and albumin and did a cell count for the lavage fluid. The predominant ironbinding protein in resting animals wastransferrin. Aerosolized E. coli caused a brisk PMNL response in the lungs that was associated with a major increase in the levels of lactoferrin. Challenge with S. aureus was associated with a moderate increase in the number of macrophages and a moderate decrease in the levels of transferrin and iron but no change in the levels of lactoferrin. The levels of iron-binding protein can vary according to the type of inflammatory response.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/154.6.959</identifier><identifier>PMID: 3537153</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Aerosols ; Albumins ; Albumins - metabolism ; Animals ; Antiserum ; Bacteria ; Bacteriology ; Biological and medical sciences ; Escherichia coli Infections - physiopathology ; Female ; Fundamental and applied biological sciences. Psychology ; Iron - metabolism ; Iron binding proteins ; Lactoferrin - metabolism ; Lactoglobulins - metabolism ; Lung - physiopathology ; Lungs ; Mice ; Microbiology ; Neutrophils - physiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Secretion ; Staphylococcal Infections - physiopathology ; Therapeutic irrigation ; Transferrin - metabolism ; Transferrins</subject><ispartof>The Journal of infectious diseases, 1986-12, Vol.154 (6), p.959-965</ispartof><rights>Copyright 1986 University of Chicago</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-d343b0c8ae74e7834d455c909e113677a96326fcbea2835687c9455e1e5c8f7c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30104979$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30104979$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8280189$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3537153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LaForce, F. Marc</creatorcontrib><creatorcontrib>Boose, Dorothy S.</creatorcontrib><creatorcontrib>Ellison, Richard T.</creatorcontrib><title>Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Iron-binding proteins have antibacterial activity; they have been identified in lung secretions, but their role in pulmonary antibacterial defenses is unclear. Murine lactoferrin and murine transferrin were used to generate polyclonal antiserum to lactoferrin and to transferrin, and the specificity of both antisera was shown by western blot. Mice were exposed to either aerosolized Escherichia coli or Staphylococcus aureus; they were killed 1, 4, 24, or 48 hr later; and their lungs were lavaged. Wemeasured the levels of transferrin, lactoferrin, and albumin and did a cell count for the lavage fluid. The predominant ironbinding protein in resting animals wastransferrin. Aerosolized E. coli caused a brisk PMNL response in the lungs that was associated with a major increase in the levels of lactoferrin. Challenge with S. aureus was associated with a moderate increase in the number of macrophages and a moderate decrease in the levels of transferrin and iron but no change in the levels of lactoferrin. The levels of iron-binding protein can vary according to the type of inflammatory response.</description><subject>Aerosols</subject><subject>Albumins</subject><subject>Albumins - metabolism</subject><subject>Animals</subject><subject>Antiserum</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli Infections - physiopathology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Iron - metabolism</subject><subject>Iron binding proteins</subject><subject>Lactoferrin - metabolism</subject><subject>Lactoglobulins - metabolism</subject><subject>Lung - physiopathology</subject><subject>Lungs</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Neutrophils - physiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Secretion</subject><subject>Staphylococcal Infections - physiopathology</subject><subject>Therapeutic irrigation</subject><subject>Transferrin - metabolism</subject><subject>Transferrins</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1vEzEQxS0EKqFw54LkA-K2qb3e9cexVAmtFAESn-JiObOzjcvGTu1doPz1OEpoLzOjeb95kp8JecnZnDMjznzoO5_PeNvM5dy05hGZ8VaoSkouHpMZY3VdcW3MU_Is5xvGWCOkOiEnokAFnJHfi75HGGns6TmmmOPg_2JHFxk2mDxsvKNQdtSFjn4a3W5zN0SIAFOmbkpYWgz0KpWyJ_ZD9daHzodr-jHFEX3I1Ae6mspi5X65a6TLYfLdc_Kkd0PGF8d-Sr4sF58vLqvVh3dXF-erCoQWY9WJRqwZaIeqQaVF0zVtC4YZ5Ly8RDkjRS17WKOrtWilVmAKgRxb0L0CcUreHHx3Kd5OmEe79RlwGFzAOGWrFFeMG15AdgChpJAT9naX_NalO8uZ3WdtD1nbkrWVtmRdTl4dvaf1Frv7g2O4RX991F0GN_TJBSgG_zFda1b-5sHmJo8xPbgwzhqj9np10H0e8c-97tJPK5VQrb38_sN-M_JrU7-v7VL8A-UWoWA</recordid><startdate>19861201</startdate><enddate>19861201</enddate><creator>LaForce, F. Marc</creator><creator>Boose, Dorothy S.</creator><creator>Ellison, Richard T.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861201</creationdate><title>Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid</title><author>LaForce, F. Marc ; Boose, Dorothy S. ; Ellison, Richard T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-d343b0c8ae74e7834d455c909e113677a96326fcbea2835687c9455e1e5c8f7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aerosols</topic><topic>Albumins</topic><topic>Albumins - metabolism</topic><topic>Animals</topic><topic>Antiserum</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Escherichia coli Infections - physiopathology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Iron - metabolism</topic><topic>Iron binding proteins</topic><topic>Lactoferrin - metabolism</topic><topic>Lactoglobulins - metabolism</topic><topic>Lung - physiopathology</topic><topic>Lungs</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Neutrophils - physiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Secretion</topic><topic>Staphylococcal Infections - physiopathology</topic><topic>Therapeutic irrigation</topic><topic>Transferrin - metabolism</topic><topic>Transferrins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LaForce, F. Marc</creatorcontrib><creatorcontrib>Boose, Dorothy S.</creatorcontrib><creatorcontrib>Ellison, Richard T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LaForce, F. Marc</au><au>Boose, Dorothy S.</au><au>Ellison, Richard T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1986-12-01</date><risdate>1986</risdate><volume>154</volume><issue>6</issue><spage>959</spage><epage>965</epage><pages>959-965</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Iron-binding proteins have antibacterial activity; they have been identified in lung secretions, but their role in pulmonary antibacterial defenses is unclear. Murine lactoferrin and murine transferrin were used to generate polyclonal antiserum to lactoferrin and to transferrin, and the specificity of both antisera was shown by western blot. Mice were exposed to either aerosolized Escherichia coli or Staphylococcus aureus; they were killed 1, 4, 24, or 48 hr later; and their lungs were lavaged. Wemeasured the levels of transferrin, lactoferrin, and albumin and did a cell count for the lavage fluid. The predominant ironbinding protein in resting animals wastransferrin. Aerosolized E. coli caused a brisk PMNL response in the lungs that was associated with a major increase in the levels of lactoferrin. Challenge with S. aureus was associated with a moderate increase in the number of macrophages and a moderate decrease in the levels of transferrin and iron but no change in the levels of lactoferrin. The levels of iron-binding protein can vary according to the type of inflammatory response.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>3537153</pmid><doi>10.1093/infdis/154.6.959</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1986-12, Vol.154 (6), p.959-965 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77170191 |
| source | Jstor Complete Legacy; MEDLINE |
| subjects | Aerosols Albumins Albumins - metabolism Animals Antiserum Bacteria Bacteriology Biological and medical sciences Escherichia coli Infections - physiopathology Female Fundamental and applied biological sciences. Psychology Iron - metabolism Iron binding proteins Lactoferrin - metabolism Lactoglobulins - metabolism Lung - physiopathology Lungs Mice Microbiology Neutrophils - physiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Secretion Staphylococcal Infections - physiopathology Therapeutic irrigation Transferrin - metabolism Transferrins |
| title | Effect of Aerosolized Escherichia coli and Staphylococcus aureus on Iron and Iron-Binding Proteins in Lung Lavage Fluid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A57%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Aerosolized%20Escherichia%20coli%20and%20Staphylococcus%20aureus%20on%20Iron%20and%20Iron-Binding%20Proteins%20in%20Lung%20Lavage%20Fluid&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=LaForce,%20F.%20Marc&rft.date=1986-12-01&rft.volume=154&rft.issue=6&rft.spage=959&rft.epage=965&rft.pages=959-965&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/10.1093/infdis/154.6.959&rft_dat=%3Cjstor_proqu%3E30104979%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77170191&rft_id=info:pmid/3537153&rft_jstor_id=30104979&rfr_iscdi=true |