In vitro recovery of human chorionic gonadotropin-stimulated cyclic adenosine 3′,5′-monophosphate production in desensitized human granulosa-luteal cells
Human granulosa-luteal cell production of cyclic adenosine 3′,5′-monophosphate (cAMP) and progesterone (P) were studied in response to purified human chorionic gonadotropin (hCG) in cultured cells from hyperstimulated follicles of in vitro fertilized patients. The hCG injection given to the patients...
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| Veröffentlicht in: | Fertility and sterility 1986-11, Vol.46 (5), p.920-924 |
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| container_title | Fertility and sterility |
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| creator | Jalkanen, Jyrki Huhtaniemi, Ilpo Koskimies, Aarne Stenman, Ulf-Håkan Tenhunen, Anssi Ranta, Tapio |
| description | Human granulosa-luteal cell production of cyclic adenosine 3′,5′-monophosphate (cAMP) and progesterone (P) were studied in response to purified human chorionic gonadotropin (hCG) in cultured cells from hyperstimulated follicles of in vitro fertilized patients. The hCG injection given to the patients 36 hours before laparoscopy caused partial desensitization of adenylate cyclase of these cells to gonadotropins. Preincubation of the cells in hormone free medium for 2 to 3 days significantly increased their cAMP responsiveness to hCG. P production was stimulated initially by hCG and showed no desensitization. In the cells preincubated for 72 hours without hCG, a subsequent stimulus of 50 ng/ml of hCG elicited maximal cAMP response, whereas 1 ng/ml of hCG was sufficient to bring about maximal P secretion. Time-course studies indicated that maximal cAMP response to hCG was obtained m I to 3 hours. Both basal and hCG-stimulated P accumulation continued to rise for up to 24 hours. Preincubation of granulosa-luteal cells from hyperstimulated follicles improves the cells’ cAMP responsiveness to hCG, whereas P response remains unaltered. |
| doi_str_mv | 10.1016/S0015-0282(16)49835-3 |
| format | Article |
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The hCG injection given to the patients 36 hours before laparoscopy caused partial desensitization of adenylate cyclase of these cells to gonadotropins. Preincubation of the cells in hormone free medium for 2 to 3 days significantly increased their cAMP responsiveness to hCG. P production was stimulated initially by hCG and showed no desensitization. In the cells preincubated for 72 hours without hCG, a subsequent stimulus of 50 ng/ml of hCG elicited maximal cAMP response, whereas 1 ng/ml of hCG was sufficient to bring about maximal P secretion. Time-course studies indicated that maximal cAMP response to hCG was obtained m I to 3 hours. Both basal and hCG-stimulated P accumulation continued to rise for up to 24 hours. Preincubation of granulosa-luteal cells from hyperstimulated follicles improves the cells’ cAMP responsiveness to hCG, whereas P response remains unaltered.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(16)49835-3</identifier><identifier>PMID: 3023146</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Birth control ; Chorionic Gonadotropin - pharmacology ; Corpus Luteum - metabolism ; Cyclic AMP - biosynthesis ; Female ; Fertilization in Vitro ; Granulosa Cells - metabolism ; Gynecology. Andrology. Obstetrics ; Humans ; Luteal Cells - metabolism ; Medical sciences ; Progesterone - biosynthesis ; Sterility. 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The hCG injection given to the patients 36 hours before laparoscopy caused partial desensitization of adenylate cyclase of these cells to gonadotropins. Preincubation of the cells in hormone free medium for 2 to 3 days significantly increased their cAMP responsiveness to hCG. P production was stimulated initially by hCG and showed no desensitization. In the cells preincubated for 72 hours without hCG, a subsequent stimulus of 50 ng/ml of hCG elicited maximal cAMP response, whereas 1 ng/ml of hCG was sufficient to bring about maximal P secretion. Time-course studies indicated that maximal cAMP response to hCG was obtained m I to 3 hours. Both basal and hCG-stimulated P accumulation continued to rise for up to 24 hours. Preincubation of granulosa-luteal cells from hyperstimulated follicles improves the cells’ cAMP responsiveness to hCG, whereas P response remains unaltered.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Corpus Luteum - metabolism</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Granulosa Cells - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Luteal Cells - metabolism</subject><subject>Medical sciences</subject><subject>Progesterone - biosynthesis</subject><subject>Sterility. 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Andrology. Obstetrics</topic><topic>Humans</topic><topic>Luteal Cells - metabolism</topic><topic>Medical sciences</topic><topic>Progesterone - biosynthesis</topic><topic>Sterility. 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The hCG injection given to the patients 36 hours before laparoscopy caused partial desensitization of adenylate cyclase of these cells to gonadotropins. Preincubation of the cells in hormone free medium for 2 to 3 days significantly increased their cAMP responsiveness to hCG. P production was stimulated initially by hCG and showed no desensitization. In the cells preincubated for 72 hours without hCG, a subsequent stimulus of 50 ng/ml of hCG elicited maximal cAMP response, whereas 1 ng/ml of hCG was sufficient to bring about maximal P secretion. Time-course studies indicated that maximal cAMP response to hCG was obtained m I to 3 hours. Both basal and hCG-stimulated P accumulation continued to rise for up to 24 hours. 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| ispartof | Fertility and sterility, 1986-11, Vol.46 (5), p.920-924 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Biological and medical sciences Birth control Chorionic Gonadotropin - pharmacology Corpus Luteum - metabolism Cyclic AMP - biosynthesis Female Fertilization in Vitro Granulosa Cells - metabolism Gynecology. Andrology. Obstetrics Humans Luteal Cells - metabolism Medical sciences Progesterone - biosynthesis Sterility. Assisted procreation Stimulation, Chemical |
| title | In vitro recovery of human chorionic gonadotropin-stimulated cyclic adenosine 3′,5′-monophosphate production in desensitized human granulosa-luteal cells |
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