Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells
We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-depe...
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| Veröffentlicht in: | FEBS letters 1995-03, Vol.360 (3), p.291-293 |
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| creator | Hishikawa, K Nakaki, T Marumo, T Suzuki, H Kato, R Saruta, T |
| description | We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens. |
| doi_str_mv | 10.1016/0014-5793(95)00124-R |
| format | Article |
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Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment was accompanied by increased NO synthase protein. 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Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.</description><subject>Amino Acid Oxidoreductases - metabolism</subject><subject>Aorta</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - enzymology</subject><subject>Enzyme Induction - drug effects</subject><subject>Estradiol - pharmacology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Molecular Sequence Data</subject><subject>Nitric Oxide Synthase</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Tamoxifen - pharmacology</subject><subject>Testosterone - pharmacology</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEYhHNQaq3-A4WcRA-ryaZJNkcpfkFBEHte3myyNpJNapIF--9dsXgaZngYhkHogpJbSqi4I4QuKy4Vu1b8ZjL1sno7QvP_-ASd5vxJJt9QNUMzyRmTtZqjzWZXJfsxeiguBhx7HFxJrsPx2xmL8z6ULWSL9R7bXBIYFz12AW_HAQKGmMrE2mBi2VrvwOPOep_P0HEPPtvzgy7Q5vHhffVcrV-fXlb366qjjSpV3SvBVd0zoKbjAoTUvWKCkaZRXAlqwIpOaCEbMNoQrcmyJ9aAFFqDqTlboKu_3l2KX-M0sB1c_l0AwcYxt1JSIYlQE3h5AEc9WNPukhsg7dvDD-wHprhfjg</recordid><startdate>19950306</startdate><enddate>19950306</enddate><creator>Hishikawa, K</creator><creator>Nakaki, T</creator><creator>Marumo, T</creator><creator>Suzuki, H</creator><creator>Kato, R</creator><creator>Saruta, T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19950306</creationdate><title>Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells</title><author>Hishikawa, K ; Nakaki, T ; Marumo, T ; Suzuki, H ; Kato, R ; Saruta, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c189t-2f96592f3a1dc56a67bf936308895961dae6c6b678adbd0bb04f0eda76bbad253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Oxidoreductases - metabolism</topic><topic>Aorta</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>Endothelium, Vascular - enzymology</topic><topic>Enzyme Induction - drug effects</topic><topic>Estradiol - pharmacology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Molecular Sequence Data</topic><topic>Nitric Oxide Synthase</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Tamoxifen - pharmacology</topic><topic>Testosterone - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hishikawa, K</creatorcontrib><creatorcontrib>Nakaki, T</creatorcontrib><creatorcontrib>Marumo, T</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Kato, R</creatorcontrib><creatorcontrib>Saruta, T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hishikawa, K</au><au>Nakaki, T</au><au>Marumo, T</au><au>Suzuki, H</au><au>Kato, R</au><au>Saruta, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1995-03-06</date><risdate>1995</risdate><volume>360</volume><issue>3</issue><spage>291</spage><epage>293</epage><pages>291-293</pages><issn>0014-5793</issn><abstract>We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. 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| ispartof | FEBS letters, 1995-03, Vol.360 (3), p.291-293 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Oxidoreductases - metabolism Aorta Base Sequence Cells, Cultured Endothelium, Vascular - enzymology Enzyme Induction - drug effects Estradiol - pharmacology Gene Expression Regulation, Enzymologic - drug effects Humans In Vitro Techniques Molecular Sequence Data Nitric Oxide Synthase Regulatory Sequences, Nucleic Acid Tamoxifen - pharmacology Testosterone - pharmacology |
| title | Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells |
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