Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus oocytes
Rat immunoglobulin E (IgE) synthesized in Xenopus laevis oocytes, injected with rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The results indicate that the oocytes will translate and c...
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| Veröffentlicht in: | FEBS letters 1986-11, Vol.208 (2), p.369-372 |
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| creator | Lund, Torben Bravo, Rodrigo Johansen, Hanne R. Zeuthen, Jesper Vuust, Jens |
| description | Rat immunoglobulin E (IgE) synthesized in
Xenopus laevis oocytes, injected with rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The results indicate that the oocytes will translate and correctly process the rat IgE heavy and light chains, resulting in secretion of a correctly assembled, normal immunoglobulin molecule. The normal, extensive glycosylation of the IgE heavy chain (ε-chain) is faithfully carried out by the oocytes; therefore, this posttranslational modification is apparently of an unspecific nature, and does not depend upon a mechanism specific for plasma cells. |
| doi_str_mv | 10.1016/0014-5793(86)81051-1 |
| format | Article |
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Xenopus laevis oocytes, injected with rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The results indicate that the oocytes will translate and correctly process the rat IgE heavy and light chains, resulting in secretion of a correctly assembled, normal immunoglobulin molecule. The normal, extensive glycosylation of the IgE heavy chain (ε-chain) is faithfully carried out by the oocytes; therefore, this posttranslational modification is apparently of an unspecific nature, and does not depend upon a mechanism specific for plasma cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(86)81051-1</identifier><identifier>PMID: 3780973</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Applied sciences ; Biological and medical sciences ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Glycosylation ; Immunoglobulin E - biosynthesis ; Immunoglobulin E - metabolism ; Immunoglobulin E Glycoprotein Posttranslational protein processing (Rat plasmacytoma ; Immunoglobulin EGlycoproteinPosttranslational protein processing(Rat plasmacytoma ; Microinjections ; Molecular and cellular biology ; Molecular genetics ; Molecular Weight ; Oocytes - metabolism ; Other techniques and industries ; Protein Biosynthesis ; Protein Processing, Post-Translational ; Rats ; RNA, Messenger - genetics ; Translation. Translation factors. Protein processing ; Xenopus laevis ; Xenopus laevis oocyte</subject><ispartof>FEBS letters, 1986-11, Vol.208 (2), p.369-372</ispartof><rights>1986</rights><rights>FEBS Letters 208 (1986) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5241-f881105b10eb34c839d9715d00feea7642d1f9f4771c4a9a623b8a6b22ca78393</citedby><cites>FETCH-LOGICAL-c5241-f881105b10eb34c839d9715d00feea7642d1f9f4771c4a9a623b8a6b22ca78393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579386810511$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8283208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8364799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3780973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lund, Torben</creatorcontrib><creatorcontrib>Bravo, Rodrigo</creatorcontrib><creatorcontrib>Johansen, Hanne R.</creatorcontrib><creatorcontrib>Zeuthen, Jesper</creatorcontrib><creatorcontrib>Vuust, Jens</creatorcontrib><title>Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus oocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Rat immunoglobulin E (IgE) synthesized in
Xenopus laevis oocytes, injected with rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The results indicate that the oocytes will translate and correctly process the rat IgE heavy and light chains, resulting in secretion of a correctly assembled, normal immunoglobulin molecule. The normal, extensive glycosylation of the IgE heavy chain (ε-chain) is faithfully carried out by the oocytes; therefore, this posttranslational modification is apparently of an unspecific nature, and does not depend upon a mechanism specific for plasma cells.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycosylation</subject><subject>Immunoglobulin E - biosynthesis</subject><subject>Immunoglobulin E - metabolism</subject><subject>Immunoglobulin E Glycoprotein Posttranslational protein processing (Rat plasmacytoma</subject><subject>Immunoglobulin EGlycoproteinPosttranslational protein processing(Rat plasmacytoma</subject><subject>Microinjections</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Weight</subject><subject>Oocytes - metabolism</subject><subject>Other techniques and industries</subject><subject>Protein Biosynthesis</subject><subject>Protein Processing, Post-Translational</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>Translation. Translation factors. Protein processing</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis oocyte</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFGL1DAUhYMo6-zqP1DIg4jCVpMmk6Qvgi4zKiz44Aq-hTS9XSNtMua2yvz7TXeGeVSfktzz3cPJIeQZZ2844-otY1xWa92IV0a9NpytecUfkBU3WlRCKvOQrE7IY3KO-JOVt-HNGTkT2rBGixW5-bqP0w_AgJd0l5MHxBBvL6mLHUXwGaaQIk09zW6iYRznmG6H1M5DiHRDR9cBLbfvENNuRpqS30-AT8ij3g0IT4_nBfm23dxcfaquv3z8fPX-uvLrWvKqN4aX2C1n0ArpjWi6RvN1x1gP4LSSdcf7ppdacy9d41QtWuNUW9fe6UKLC_Ly4FuS_5oBJzsG9DAMLkKa0ZZFpbjSBZQH0OeEmKG3uxxGl_eWM7uUaZem7NKUNcrel2l5WXt-9J_bEbrT0rG9or846g69G_rsog94woxQUjfNP7HaiJqZgm0P2J8wwP6_Etrt5kO9CMvcqPvpEvvdwQhK978DZIs-QPTQhQx-sl0Kf__3Hel1r0s</recordid><startdate>19861124</startdate><enddate>19861124</enddate><creator>Lund, Torben</creator><creator>Bravo, Rodrigo</creator><creator>Johansen, Hanne R.</creator><creator>Zeuthen, Jesper</creator><creator>Vuust, Jens</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861124</creationdate><title>Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus oocytes</title><author>Lund, Torben ; Bravo, Rodrigo ; Johansen, Hanne R. ; Zeuthen, Jesper ; Vuust, Jens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5241-f881105b10eb34c839d9715d00feea7642d1f9f4771c4a9a623b8a6b22ca78393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycosylation</topic><topic>Immunoglobulin E - biosynthesis</topic><topic>Immunoglobulin E - metabolism</topic><topic>Immunoglobulin E Glycoprotein Posttranslational protein processing (Rat plasmacytoma</topic><topic>Immunoglobulin EGlycoproteinPosttranslational protein processing(Rat plasmacytoma</topic><topic>Microinjections</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Weight</topic><topic>Oocytes - metabolism</topic><topic>Other techniques and industries</topic><topic>Protein Biosynthesis</topic><topic>Protein Processing, Post-Translational</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>Translation. Translation factors. Protein processing</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis oocyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lund, Torben</creatorcontrib><creatorcontrib>Bravo, Rodrigo</creatorcontrib><creatorcontrib>Johansen, Hanne R.</creatorcontrib><creatorcontrib>Zeuthen, Jesper</creatorcontrib><creatorcontrib>Vuust, Jens</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lund, Torben</au><au>Bravo, Rodrigo</au><au>Johansen, Hanne R.</au><au>Zeuthen, Jesper</au><au>Vuust, Jens</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus oocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986-11-24</date><risdate>1986</risdate><volume>208</volume><issue>2</issue><spage>369</spage><epage>372</epage><pages>369-372</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Rat immunoglobulin E (IgE) synthesized in
Xenopus laevis oocytes, injected with rat plasmacytoma mRNA, was analysed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The results indicate that the oocytes will translate and correctly process the rat IgE heavy and light chains, resulting in secretion of a correctly assembled, normal immunoglobulin molecule. The normal, extensive glycosylation of the IgE heavy chain (ε-chain) is faithfully carried out by the oocytes; therefore, this posttranslational modification is apparently of an unspecific nature, and does not depend upon a mechanism specific for plasma cells.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3780973</pmid><doi>10.1016/0014-5793(86)81051-1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Applied sciences Biological and medical sciences Exact sciences and technology Fundamental and applied biological sciences. Psychology Glycosylation Immunoglobulin E - biosynthesis Immunoglobulin E - metabolism Immunoglobulin E Glycoprotein Posttranslational protein processing (Rat plasmacytoma Immunoglobulin EGlycoproteinPosttranslational protein processing(Rat plasmacytoma Microinjections Molecular and cellular biology Molecular genetics Molecular Weight Oocytes - metabolism Other techniques and industries Protein Biosynthesis Protein Processing, Post-Translational Rats RNA, Messenger - genetics Translation. Translation factors. Protein processing Xenopus laevis Xenopus laevis oocyte |
| title | Synthesis, processing, and secretion of rat immunoglobulin E made in Xenopus oocytes |
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