Purification and Characterization of Lipoprotein Lipase from Human Postheparin Plasma and Its Comparison with Purified Bovine Milk Lipoprotein Lipase

Lipoprotein lipase [EC 3.1.1.34, LpL] was purified from human postheparin plasma (PHP) almost to homogeneity (a 210,000-fold purification) using columns of heparin-Sepharose, hydroxylapatite, and concanavalin A-Sepharose, and its properties were compared with the purified bovine milk LpL. The specif...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1986, Vol.100 (2), p.319-331
Hauptverfasser:	HAYASHI, Rikurou, TAJIMA, Shoji, YAMAMOTO, Akira
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Enzymes and enzyme inhibitors Fatty Acids, Nonesterified - metabolism Female Fundamental and applied biological sciences. Psychology Heparin Humans Hydrolases Kinetics lipoprotein lipase Lipoprotein Lipase - blood Lipoprotein Lipase - isolation & purification man milk Milk - enzymology Molecular Weight Plasma
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