Characterization of a Negative cis-Acting DNA Element Regulating the Transcription of CYP2B1/B2 Gene in Rat Liver
The region −160 to −127 nt of the upstream of CYP-2B1/B2 gene has been found to function as a negative cis-acting element on the basis of DNase-I footprint and gel mobility shift assays as well as cell-free transcriptional assays using Bal-31 mutants. A reciprocal relationship in the interaction of...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1995-02, Vol.317 (1), p.39-45 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Ram, N. Rao, M.V. Prabhu, L. Nirodi, C.S. Sultana, S. Vatsala, P.G. Padmanaban, G. |
| description | The region −160 to −127 nt of the upstream of CYP-2B1/B2 gene has been found to function as a negative
cis-acting element on the basis of DNase-I footprint and gel mobility shift assays as well as cell-free transcriptional assays using Bal-31 mutants. A reciprocal relationship in the interaction of the negative and the recently characterized positive elements with their respective protein factors has been found under repressed and induced conditions of the gene. The negative element also harbors the core glucocorticoid responsive sequence, TGTCCT. It is concluded that the negative element mediates the repressed state of the gene under the uninduced condition and also mediates the repressive effect of dexamethasone, when given along with the inducer phenobarbitone in rats. Dexamethasone is able to antagonize the effects of phenobarbitone at as low a concentration as 100 μg/kg body wt in these animals. |
| doi_str_mv | 10.1006/abbi.1995.1133 |
| format | Article |
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cis-acting element on the basis of DNase-I footprint and gel mobility shift assays as well as cell-free transcriptional assays using Bal-31 mutants. A reciprocal relationship in the interaction of the negative and the recently characterized positive elements with their respective protein factors has been found under repressed and induced conditions of the gene. The negative element also harbors the core glucocorticoid responsive sequence, TGTCCT. It is concluded that the negative element mediates the repressed state of the gene under the uninduced condition and also mediates the repressive effect of dexamethasone, when given along with the inducer phenobarbitone in rats. Dexamethasone is able to antagonize the effects of phenobarbitone at as low a concentration as 100 μg/kg body wt in these animals.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1006/abbi.1995.1133</identifier><identifier>PMID: 7872801</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Dexamethasone - pharmacology ; DNA - analysis ; DNA Fingerprinting ; DNA Primers ; Enhancer Elements, Genetic ; Gene Expression Regulation ; Liver - metabolism ; Male ; Molecular Sequence Data ; Phenobarbital - pharmacology ; Rats ; Rats, Wistar ; Transcription, Genetic</subject><ispartof>Archives of biochemistry and biophysics, 1995-02, Vol.317 (1), p.39-45</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-679e781d895e3c45c11c39cdfd9564c4ae2a45cef68a18eef6c47fb76bf1adec3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abbi.1995.1133$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7872801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ram, N.</creatorcontrib><creatorcontrib>Rao, M.V.</creatorcontrib><creatorcontrib>Prabhu, L.</creatorcontrib><creatorcontrib>Nirodi, C.S.</creatorcontrib><creatorcontrib>Sultana, S.</creatorcontrib><creatorcontrib>Vatsala, P.G.</creatorcontrib><creatorcontrib>Padmanaban, G.</creatorcontrib><title>Characterization of a Negative cis-Acting DNA Element Regulating the Transcription of CYP2B1/B2 Gene in Rat Liver</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The region −160 to −127 nt of the upstream of CYP-2B1/B2 gene has been found to function as a negative
cis-acting element on the basis of DNase-I footprint and gel mobility shift assays as well as cell-free transcriptional assays using Bal-31 mutants. A reciprocal relationship in the interaction of the negative and the recently characterized positive elements with their respective protein factors has been found under repressed and induced conditions of the gene. The negative element also harbors the core glucocorticoid responsive sequence, TGTCCT. It is concluded that the negative element mediates the repressed state of the gene under the uninduced condition and also mediates the repressive effect of dexamethasone, when given along with the inducer phenobarbitone in rats. Dexamethasone is able to antagonize the effects of phenobarbitone at as low a concentration as 100 μg/kg body wt in these animals.</description><subject>Animals</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Dexamethasone - pharmacology</subject><subject>DNA - analysis</subject><subject>DNA Fingerprinting</subject><subject>DNA Primers</subject><subject>Enhancer Elements, Genetic</subject><subject>Gene Expression Regulation</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Phenobarbital - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Transcription, Genetic</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P3DAQhq0KRBfotbdKPnHL4okTxz4uW76kFa0QPfRkOZPJ4iqbLLYXqfx6suzCjdNoZt55pHkY-w5iCkKoc1fXfgrGlFMAKb-wCQijMiF1ccAmQgiZGa3gKzuO8Z8QAIXKj9hRpatcC5iwp_mjCw4TBf_ikh96PrTc8Ttajt0zcfQxm2Hy_ZL_vJvxy45W1Cd-T8tN597G6ZH4Q3B9xODX74T539_5BZxf5PyaeuK-5_cu8cVIDKfssHVdpG_7esL-XF0-zG-yxa_r2_lskaGUJmWqMlRpaLQpSWJRIgBKg03bmFIVWDjK3TilVmkHmsaKRdXWlapbcA2hPGFnO-46DE8bismufETqOtfTsIm2qkDlqtRjcLoLYhhiDNTadfArF_5bEHbr2G4d261ju3U8HvzYkzf1ipqP-F7quNe7PY3vPXsKNqKnHqnxgTDZZvCfoV8BiJSK4Q</recordid><startdate>19950220</startdate><enddate>19950220</enddate><creator>Ram, N.</creator><creator>Rao, M.V.</creator><creator>Prabhu, L.</creator><creator>Nirodi, C.S.</creator><creator>Sultana, S.</creator><creator>Vatsala, P.G.</creator><creator>Padmanaban, G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950220</creationdate><title>Characterization of a Negative cis-Acting DNA Element Regulating the Transcription of CYP2B1/B2 Gene in Rat Liver</title><author>Ram, N. ; Rao, M.V. ; Prabhu, L. ; Nirodi, C.S. ; Sultana, S. ; Vatsala, P.G. ; Padmanaban, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-679e781d895e3c45c11c39cdfd9564c4ae2a45cef68a18eef6c47fb76bf1adec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Dexamethasone - pharmacology</topic><topic>DNA - analysis</topic><topic>DNA Fingerprinting</topic><topic>DNA Primers</topic><topic>Enhancer Elements, Genetic</topic><topic>Gene Expression Regulation</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Phenobarbital - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ram, N.</creatorcontrib><creatorcontrib>Rao, M.V.</creatorcontrib><creatorcontrib>Prabhu, L.</creatorcontrib><creatorcontrib>Nirodi, C.S.</creatorcontrib><creatorcontrib>Sultana, S.</creatorcontrib><creatorcontrib>Vatsala, P.G.</creatorcontrib><creatorcontrib>Padmanaban, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ram, N.</au><au>Rao, M.V.</au><au>Prabhu, L.</au><au>Nirodi, C.S.</au><au>Sultana, S.</au><au>Vatsala, P.G.</au><au>Padmanaban, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a Negative cis-Acting DNA Element Regulating the Transcription of CYP2B1/B2 Gene in Rat Liver</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1995-02-20</date><risdate>1995</risdate><volume>317</volume><issue>1</issue><spage>39</spage><epage>45</epage><pages>39-45</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The region −160 to −127 nt of the upstream of CYP-2B1/B2 gene has been found to function as a negative
cis-acting element on the basis of DNase-I footprint and gel mobility shift assays as well as cell-free transcriptional assays using Bal-31 mutants. A reciprocal relationship in the interaction of the negative and the recently characterized positive elements with their respective protein factors has been found under repressed and induced conditions of the gene. The negative element also harbors the core glucocorticoid responsive sequence, TGTCCT. It is concluded that the negative element mediates the repressed state of the gene under the uninduced condition and also mediates the repressive effect of dexamethasone, when given along with the inducer phenobarbitone in rats. Dexamethasone is able to antagonize the effects of phenobarbitone at as low a concentration as 100 μg/kg body wt in these animals.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7872801</pmid><doi>10.1006/abbi.1995.1133</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1995-02, Vol.317 (1), p.39-45 |
| issn | 0003-9861 1096-0384 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Dexamethasone - pharmacology DNA - analysis DNA Fingerprinting DNA Primers Enhancer Elements, Genetic Gene Expression Regulation Liver - metabolism Male Molecular Sequence Data Phenobarbital - pharmacology Rats Rats, Wistar Transcription, Genetic |
| title | Characterization of a Negative cis-Acting DNA Element Regulating the Transcription of CYP2B1/B2 Gene in Rat Liver |
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