Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells
Vasoactive intestinal peptide (VIP) was originally isolated from porcine duodenum and considered to be a gut hormone. Recent evidence indicates that it may also be involved in reproductive functions. In this study, a possible action of VIP on steroidogenesis by cultured testicular cells was investig...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1986-11, Vol.48 (1), p.21-29 |
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| creator | Kasson, Barry G. Lim, Paul Hsueh, Aaron J.W. |
| description | Vasoactive intestinal peptide (VIP) was originally isolated from porcine duodenum and considered to be a gut hormone. Recent evidence indicates that it may also be involved in reproductive functions. In this study, a possible action of VIP on steroidogenesis by cultured testicular cells was investigated. Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay. Treatment of cultured cells with VIP (10
−9 to 10
−6 M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion. Testosterone production in response to 10
−6 M VIP was about 5–10% of that maximally induced by LH. Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone. Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP. The VIP-related hormones PHM-27, secretin, and glucagon also stimulated progesterone and testosterone production with a potency order (PHM-27 > secretin > glucagon) consistent with that observed for other VIP receptor-mediated actions. A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient. In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells but failed to affect steroid production in non-LH-responsive cells. Thus, these experiments demonstrate that VIP, independent of LH, stimulates steroidogenesis in cultured neonatal testicular cells and suggest a possible stimulatory role for VIP in testicular steroidogenesis by the developing testis. |
| doi_str_mv | 10.1016/0303-7207(86)90162-0 |
| format | Article |
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−9 to 10
−6 M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion. Testosterone production in response to 10
−6 M VIP was about 5–10% of that maximally induced by LH. Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone. Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP. The VIP-related hormones PHM-27, secretin, and glucagon also stimulated progesterone and testosterone production with a potency order (PHM-27 > secretin > glucagon) consistent with that observed for other VIP receptor-mediated actions. A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient. In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells but failed to affect steroid production in non-LH-responsive cells. Thus, these experiments demonstrate that VIP, independent of LH, stimulates steroidogenesis in cultured neonatal testicular cells and suggest a possible stimulatory role for VIP in testicular steroidogenesis by the developing testis.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(86)90162-0</identifier><identifier>PMID: 2430845</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; 3-Hydroxysteroid Dehydrogenases - metabolism ; androgens ; Animals ; Animals, Newborn - metabolism ; Biological and medical sciences ; Cell Separation ; Cells, Cultured ; Cyclic AMP - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Glucagon - pharmacology ; Hormone metabolism and regulation ; Leydig cells ; Luteinizing Hormone - pharmacology ; Male ; Mammalian male genital system ; Peptide PHI - pharmacology ; Pregnenolone - biosynthesis ; Progesterone - biosynthesis ; Rats ; Rats, Inbred Strains ; Secretin - pharmacology ; Testis - drug effects ; Testis - metabolism ; Testosterone - biosynthesis ; vasoactive intestinal peptide ; Vasoactive Intestinal Peptide - pharmacology ; Vertebrates: reproduction</subject><ispartof>Molecular and cellular endocrinology, 1986-11, Vol.48 (1), p.21-29</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-81f169649b897338fb77050cc9cbeba5d3411619b006ddfec523663e9cf7ef4a3</citedby><cites>FETCH-LOGICAL-c417t-81f169649b897338fb77050cc9cbeba5d3411619b006ddfec523663e9cf7ef4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0303720786901620$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8206500$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2430845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasson, Barry G.</creatorcontrib><creatorcontrib>Lim, Paul</creatorcontrib><creatorcontrib>Hsueh, Aaron J.W.</creatorcontrib><title>Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Vasoactive intestinal peptide (VIP) was originally isolated from porcine duodenum and considered to be a gut hormone. Recent evidence indicates that it may also be involved in reproductive functions. In this study, a possible action of VIP on steroidogenesis by cultured testicular cells was investigated. Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay. Treatment of cultured cells with VIP (10
−9 to 10
−6 M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion. Testosterone production in response to 10
−6 M VIP was about 5–10% of that maximally induced by LH. Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone. Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP. The VIP-related hormones PHM-27, secretin, and glucagon also stimulated progesterone and testosterone production with a potency order (PHM-27 > secretin > glucagon) consistent with that observed for other VIP receptor-mediated actions. A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient. In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells but failed to affect steroid production in non-LH-responsive cells. Thus, these experiments demonstrate that VIP, independent of LH, stimulates steroidogenesis in cultured neonatal testicular cells and suggest a possible stimulatory role for VIP in testicular steroidogenesis by the developing testis.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>3-Hydroxysteroid Dehydrogenases - metabolism</subject><subject>androgens</subject><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucagon - pharmacology</subject><subject>Hormone metabolism and regulation</subject><subject>Leydig cells</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Peptide PHI - pharmacology</subject><subject>Pregnenolone - biosynthesis</subject><subject>Progesterone - biosynthesis</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Secretin - pharmacology</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testosterone - biosynthesis</subject><subject>vasoactive intestinal peptide</subject><subject>Vasoactive Intestinal Peptide - pharmacology</subject><subject>Vertebrates: reproduction</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVJSLfb_oMUdAihPbgdWbYkXwIhtE0gkEvbq5ClcarilTeSHNh_Xzm77DE9Cb355vF4Q8g5gy8MmPgKHHgla5CflPjcFaWu4A1ZMSXrSkErT8jqiLwl71L6CwCyrdUZOasbDqppV8T9NmkyNvtnpD5kTNkHM9ItbrN3SMt3M4-m6NQEF6dHDLT3U9qF_AeTT7TfUTuPeY7oaMApmFy2X2yKbCK1OI7pPTkdzJjww-Fdk1_fv_28ua3uH37c3VzfV7ZhMleKDUx0oul61UnO1dBLCS1Y29kee9M63jAmWNcDCOcGtG3NheDY2UHi0Bi-Jpd7322cnuYSQm98WhKYEm1OWspSUqu6_4KsEUwyJQrY7EEbp5QiDnob_cbEnWaglyvopWK9VKyV0C9XKNKafDz4z_0G3XHpUHuZXxzmJlkzDtEE69MRUzWIFhabqz2GpbRnj1En6zFYdD6izdpN_vUc_wBInqUK</recordid><startdate>19861101</startdate><enddate>19861101</enddate><creator>Kasson, Barry G.</creator><creator>Lim, Paul</creator><creator>Hsueh, Aaron J.W.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19861101</creationdate><title>Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells</title><author>Kasson, Barry G. ; Lim, Paul ; Hsueh, Aaron J.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-81f169649b897338fb77050cc9cbeba5d3411619b006ddfec523663e9cf7ef4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>3-Hydroxysteroid Dehydrogenases - metabolism</topic><topic>androgens</topic><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucagon - pharmacology</topic><topic>Hormone metabolism and regulation</topic><topic>Leydig cells</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Peptide PHI - pharmacology</topic><topic>Pregnenolone - biosynthesis</topic><topic>Progesterone - biosynthesis</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Secretin - pharmacology</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testosterone - biosynthesis</topic><topic>vasoactive intestinal peptide</topic><topic>Vasoactive Intestinal Peptide - pharmacology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasson, Barry G.</creatorcontrib><creatorcontrib>Lim, Paul</creatorcontrib><creatorcontrib>Hsueh, Aaron J.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasson, Barry G.</au><au>Lim, Paul</au><au>Hsueh, Aaron J.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1986-11-01</date><risdate>1986</risdate><volume>48</volume><issue>1</issue><spage>21</spage><epage>29</epage><pages>21-29</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>Vasoactive intestinal peptide (VIP) was originally isolated from porcine duodenum and considered to be a gut hormone. Recent evidence indicates that it may also be involved in reproductive functions. In this study, a possible action of VIP on steroidogenesis by cultured testicular cells was investigated. Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay. Treatment of cultured cells with VIP (10
−9 to 10
−6 M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion. Testosterone production in response to 10
−6 M VIP was about 5–10% of that maximally induced by LH. Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone. Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP. The VIP-related hormones PHM-27, secretin, and glucagon also stimulated progesterone and testosterone production with a potency order (PHM-27 > secretin > glucagon) consistent with that observed for other VIP receptor-mediated actions. A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient. In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells but failed to affect steroid production in non-LH-responsive cells. Thus, these experiments demonstrate that VIP, independent of LH, stimulates steroidogenesis in cultured neonatal testicular cells and suggest a possible stimulatory role for VIP in testicular steroidogenesis by the developing testis.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>2430845</pmid><doi>10.1016/0303-7207(86)90162-0</doi><tpages>9</tpages></addata></record> |
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| issn | 0303-7207 1872-8057 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology 3-Hydroxysteroid Dehydrogenases - metabolism androgens Animals Animals, Newborn - metabolism Biological and medical sciences Cell Separation Cells, Cultured Cyclic AMP - biosynthesis Fundamental and applied biological sciences. Psychology Glucagon - pharmacology Hormone metabolism and regulation Leydig cells Luteinizing Hormone - pharmacology Male Mammalian male genital system Peptide PHI - pharmacology Pregnenolone - biosynthesis Progesterone - biosynthesis Rats Rats, Inbred Strains Secretin - pharmacology Testis - drug effects Testis - metabolism Testosterone - biosynthesis vasoactive intestinal peptide Vasoactive Intestinal Peptide - pharmacology Vertebrates: reproduction |
| title | Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells |
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