Microbial transformation of steroids—IX. Purification of progesterone hydroxylase cytochrome P-450 from Phycomyces blakesleeanus

Progesterone hydroxylase cytochrome P-450 was purified to homogeneity from Phycomyces blakesleeanus microsomes by a four step procedure. An M r value of 60,000 was determined for this protein by SDS-PAGE. The DEAE-cellulose and Blue-1 MIMETIC affinity fractions gave major peaks at 452 nm in a dithio...

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Veröffentlicht in:	The Journal of steroid biochemistry and molecular biology 1995, Vol.52 (2), p.203-208
Hauptverfasser:	Ahmed, Farjad, Williams, Ralph A.D., Smith, Kelvin E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Chromatography, Affinity Chromatography, DEAE-Cellulose Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - isolation & purification Cytochrome P-450 Enzyme System - metabolism Fundamental and applied biological sciences. Psychology Hydroxylation Microsomes - enzymology Mission oriented research Molecular Weight NADP - metabolism Periodic Acid - metabolism Phycomyces - enzymology Phycomyces blakesleeanus Physiology and metabolism Progesterone - metabolism Steroid Hydroxylases - chemistry Steroid Hydroxylases - isolation & purification Steroid Hydroxylases - metabolism
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