Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography
A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 μ1 human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC c...
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| Veröffentlicht in: | Current eye research 1986, Vol.5 (11), p.841-845 |
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| container_issue | 11 |
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| container_title | Current eye research |
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| creator | Speek, Andries J. van Agtmaal, Eric J. Saowakontha, Sastri Schreurs, Wil H.P. van Haeringen, Nicolaas J. |
| description | A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 μ1 human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A can be determined in concentrations as low as 0.4 ng/ml. A single analysis can be completed in 12 min while the analysis of a series of 60 samples takes about 8 h. The within-assay and between-assay coefficients of variation were 3.1 and 4.2 % resp. The between-assay analytical recovery of retinol added to tear fluid was 97.4 ± 6.0 % (mean ± SD). Retinol was determined in tear fluid of nine adult volunteers. The amounts observed ranged from < 0.4 - 10.6 ng/ml. |
| doi_str_mv | 10.3109/02713688609029235 |
| format | Article |
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After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A can be determined in concentrations as low as 0.4 ng/ml. A single analysis can be completed in 12 min while the analysis of a series of 60 samples takes about 8 h. The within-assay and between-assay coefficients of variation were 3.1 and 4.2 % resp. The between-assay analytical recovery of retinol added to tear fluid was 97.4 ± 6.0 % (mean ± SD). Retinol was determined in tear fluid of nine adult volunteers. 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Methods ; Humans ; Tears - metabolism ; Vertebrates: nervous system and sense organs ; Vitamin A - metabolism</subject><ispartof>Current eye research, 1986, Vol.5 (11), p.841-845</ispartof><rights>1986 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-e364e5a0f291e8da0301d9a6ba17570ba58124502f35703982b9bc94abfcd4e13</citedby><cites>FETCH-LOGICAL-c430t-e364e5a0f291e8da0301d9a6ba17570ba58124502f35703982b9bc94abfcd4e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/02713688609029235$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/02713688609029235$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7961234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3780282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Speek, Andries J.</creatorcontrib><creatorcontrib>van Agtmaal, Eric J.</creatorcontrib><creatorcontrib>Saowakontha, Sastri</creatorcontrib><creatorcontrib>Schreurs, Wil H.P.</creatorcontrib><creatorcontrib>van Haeringen, Nicolaas J.</creatorcontrib><title>Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 μ1 human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A can be determined in concentrations as low as 0.4 ng/ml. A single analysis can be completed in 12 min while the analysis of a series of 60 samples takes about 8 h. The within-assay and between-assay coefficients of variation were 3.1 and 4.2 % resp. The between-assay analytical recovery of retinol added to tear fluid was 97.4 ± 6.0 % (mean ± SD). Retinol was determined in tear fluid of nine adult volunteers. The amounts observed ranged from < 0.4 - 10.6 ng/ml.</description><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, High Pressure Liquid - standards</subject><subject>Drug Stability</subject><subject>Evaluation Studies as Topic</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Humans</topic><topic>Tears - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vitamin A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Speek, Andries J.</creatorcontrib><creatorcontrib>van Agtmaal, Eric J.</creatorcontrib><creatorcontrib>Saowakontha, Sastri</creatorcontrib><creatorcontrib>Schreurs, Wil H.P.</creatorcontrib><creatorcontrib>van Haeringen, Nicolaas J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Speek, Andries J.</au><au>van Agtmaal, Eric J.</au><au>Saowakontha, Sastri</au><au>Schreurs, Wil H.P.</au><au>van Haeringen, Nicolaas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>1986</date><risdate>1986</risdate><volume>5</volume><issue>11</issue><spage>841</spage><epage>845</epage><pages>841-845</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><coden>CEYRDM</coden><abstract>A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 μ1 human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A can be determined in concentrations as low as 0.4 ng/ml. A single analysis can be completed in 12 min while the analysis of a series of 60 samples takes about 8 h. The within-assay and between-assay coefficients of variation were 3.1 and 4.2 % resp. The between-assay analytical recovery of retinol added to tear fluid was 97.4 ± 6.0 % (mean ± SD). Retinol was determined in tear fluid of nine adult volunteers. The amounts observed ranged from < 0.4 - 10.6 ng/ml.</abstract><cop>Lisse</cop><pub>Informa UK Ltd</pub><pmid>3780282</pmid><doi>10.3109/02713688609029235</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0271-3683 |
| ispartof | Current eye research, 1986, Vol.5 (11), p.841-845 |
| issn | 0271-3683 1460-2202 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77157790 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Biological and medical sciences Chromatography, High Pressure Liquid - methods Chromatography, High Pressure Liquid - standards Drug Stability Evaluation Studies as Topic Fluorometry Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Humans Tears - metabolism Vertebrates: nervous system and sense organs Vitamin A - metabolism |
| title | Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography |
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