The effect of proteoglycans, collagen and lysyl oxidase on the metabolism of low density lipoprotein by macrophages
To study the interactions of lipoproteins, connective tissue components and cells, mouse peritoneal macrophages were incubated in the presence of human low density lipoproteins (LDL) that had been complexed with pig aortic proteoglycans (PG) or incubated in the presence of soluble collagen and/or ly...
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| Veröffentlicht in: | Atherosclerosis 1986-10, Vol.62 (1), p.73-80 |
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| creator | Ylä-Herttuala, Seppo Jaakkola, Olli Solakivi, Tiina Kuivaniemi, Helena Nikkari, Tapio |
| description | To study the interactions of lipoproteins, connective tissue components and cells, mouse peritoneal macrophages were incubated in the presence of human low density lipoproteins (LDL) that had been complexed with pig aortic proteoglycans (PG) or incubated in the presence of soluble collagen and/or lysyl oxidase, which catalyses the formation of cross-linkages in collagen and elastin by oxidising ϵ-amino groups of lysine residues to aldehydes.
Soluble and insoluble PG-LDL complexes increased the incorporation of [
3H]oleate into cellular cholesteryl esters (CE) 1.6- and 2.8-fold, respectively, while LDL incubated with collagen and lysyl oxidase had no effect compared to control LDL. As judged on the basis of incubations with fucoidin, spermine and
125I-labelled lipoproteins, the mechanism of internalisation of the PG-LDL complexes is different from that of acetylated LDL or dextran sulphate-LDL complexes.
The formation of PG-LDL complexes in the arterial intima may lead to an increased uptake of lipoproteins by intimal macrophages during the early phase of atherogenesis. |
| doi_str_mv | 10.1016/0021-9150(86)90021-3 |
| format | Article |
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Soluble and insoluble PG-LDL complexes increased the incorporation of [
3H]oleate into cellular cholesteryl esters (CE) 1.6- and 2.8-fold, respectively, while LDL incubated with collagen and lysyl oxidase had no effect compared to control LDL. As judged on the basis of incubations with fucoidin, spermine and
125I-labelled lipoproteins, the mechanism of internalisation of the PG-LDL complexes is different from that of acetylated LDL or dextran sulphate-LDL complexes.
The formation of PG-LDL complexes in the arterial intima may lead to an increased uptake of lipoproteins by intimal macrophages during the early phase of atherogenesis.</description><identifier>ISSN: 0021-9150</identifier><identifier>EISSN: 1879-1484</identifier><identifier>DOI: 10.1016/0021-9150(86)90021-3</identifier><identifier>PMID: 2877675</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ireland Ltd</publisher><subject>Amino Acid Oxidoreductases - metabolism ; Animals ; Aorta ; Atherogenesis ; Biological and medical sciences ; Cell cultures ; Cells, Cultured ; Cholesterol Esters - biosynthesis ; Collagen ; Collagen - pharmacology ; Disorders of blood lipids. Hyperlipoproteinemia ; Humans ; Kinetics ; LDL ; Lipoproteins, LDL - blood ; Lipoproteins, LDL - metabolism ; Lysyl oxidase ; Macrophages ; Macrophages - drug effects ; Macrophages - metabolism ; Male ; Medical sciences ; Metabolic diseases ; Mice ; Mice, Inbred Strains ; Protein-Lysine 6-Oxidase - metabolism ; Proteoglycans ; Proteoglycans - isolation & purification ; Proteoglycans - pharmacology ; Swine</subject><ispartof>Atherosclerosis, 1986-10, Vol.62 (1), p.73-80</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-b3d747638a525b57df9dd2f3a1083729eef622d62d0b699767c138a5004bcb183</citedby><cites>FETCH-LOGICAL-c452t-b3d747638a525b57df9dd2f3a1083729eef622d62d0b699767c138a5004bcb183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0021-9150(86)90021-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8188447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2877675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><creatorcontrib>Jaakkola, Olli</creatorcontrib><creatorcontrib>Solakivi, Tiina</creatorcontrib><creatorcontrib>Kuivaniemi, Helena</creatorcontrib><creatorcontrib>Nikkari, Tapio</creatorcontrib><title>The effect of proteoglycans, collagen and lysyl oxidase on the metabolism of low density lipoprotein by macrophages</title><title>Atherosclerosis</title><addtitle>Atherosclerosis</addtitle><description>To study the interactions of lipoproteins, connective tissue components and cells, mouse peritoneal macrophages were incubated in the presence of human low density lipoproteins (LDL) that had been complexed with pig aortic proteoglycans (PG) or incubated in the presence of soluble collagen and/or lysyl oxidase, which catalyses the formation of cross-linkages in collagen and elastin by oxidising ϵ-amino groups of lysine residues to aldehydes.
Soluble and insoluble PG-LDL complexes increased the incorporation of [
3H]oleate into cellular cholesteryl esters (CE) 1.6- and 2.8-fold, respectively, while LDL incubated with collagen and lysyl oxidase had no effect compared to control LDL. As judged on the basis of incubations with fucoidin, spermine and
125I-labelled lipoproteins, the mechanism of internalisation of the PG-LDL complexes is different from that of acetylated LDL or dextran sulphate-LDL complexes.
The formation of PG-LDL complexes in the arterial intima may lead to an increased uptake of lipoproteins by intimal macrophages during the early phase of atherogenesis.</description><subject>Amino Acid Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>Aorta</subject><subject>Atherogenesis</subject><subject>Biological and medical sciences</subject><subject>Cell cultures</subject><subject>Cells, Cultured</subject><subject>Cholesterol Esters - biosynthesis</subject><subject>Collagen</subject><subject>Collagen - pharmacology</subject><subject>Disorders of blood lipids. Hyperlipoproteinemia</subject><subject>Humans</subject><subject>Kinetics</subject><subject>LDL</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>Lysyl oxidase</subject><subject>Macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Protein-Lysine 6-Oxidase - metabolism</subject><subject>Proteoglycans</subject><subject>Proteoglycans - isolation & purification</subject><subject>Proteoglycans - pharmacology</subject><subject>Swine</subject><issn>0021-9150</issn><issn>1879-1484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9rFDEUxYNY6rb6DRTyIFKho_kzk2ReBClqhYIv9Tlkkps2kknWyax2vr2Z3WUffQrh_s7hnnMRek3JB0qo-EgIo01PO3KlxPt-_-PP0IYq2Te0Ve1ztDkhL9BFKb8IIa2k6hydMyWlkN0GlftHwOA92Blnj7dTniE_xMWaVK6xzTGaB0jYJIfjUpaI81NwpgDOCc9VOsJshhxDGVd5zH-xg1TCvOAYtnlvFxIeFjwaO-XtY3UrL9GZN7HAq-N7iX5-_XJ_c9vc_fj2_ebzXWPbjs3NwJ1speDKdKwbOul87xzz3FCiuGQ9gBeMOcEcGUTf1zyWrnANOdiBKn6J3h186xq_d1BmPYZioUZKkHdFS0k7LnhfwfYA1hVLmcDr7RRGMy2aEr12rdci9VqkVkLvu9a8yt4c_XfDCO4kOpZb52-Pc1OsiX4yyYZywhRVqm1lxT4dMKhd_Akw6WIDJAsuTPUs2uXw_z3-AQaVm2o</recordid><startdate>19861001</startdate><enddate>19861001</enddate><creator>Ylä-Herttuala, Seppo</creator><creator>Jaakkola, Olli</creator><creator>Solakivi, Tiina</creator><creator>Kuivaniemi, Helena</creator><creator>Nikkari, Tapio</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861001</creationdate><title>The effect of proteoglycans, collagen and lysyl oxidase on the metabolism of low density lipoprotein by macrophages</title><author>Ylä-Herttuala, Seppo ; Jaakkola, Olli ; Solakivi, Tiina ; Kuivaniemi, Helena ; Nikkari, Tapio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-b3d747638a525b57df9dd2f3a1083729eef622d62d0b699767c138a5004bcb183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Amino Acid Oxidoreductases - metabolism</topic><topic>Animals</topic><topic>Aorta</topic><topic>Atherogenesis</topic><topic>Biological and medical sciences</topic><topic>Cell cultures</topic><topic>Cells, Cultured</topic><topic>Cholesterol Esters - biosynthesis</topic><topic>Collagen</topic><topic>Collagen - pharmacology</topic><topic>Disorders of blood lipids. Hyperlipoproteinemia</topic><topic>Humans</topic><topic>Kinetics</topic><topic>LDL</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>Lysyl oxidase</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Protein-Lysine 6-Oxidase - metabolism</topic><topic>Proteoglycans</topic><topic>Proteoglycans - isolation & purification</topic><topic>Proteoglycans - pharmacology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><creatorcontrib>Jaakkola, Olli</creatorcontrib><creatorcontrib>Solakivi, Tiina</creatorcontrib><creatorcontrib>Kuivaniemi, Helena</creatorcontrib><creatorcontrib>Nikkari, Tapio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Atherosclerosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ylä-Herttuala, Seppo</au><au>Jaakkola, Olli</au><au>Solakivi, Tiina</au><au>Kuivaniemi, Helena</au><au>Nikkari, Tapio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of proteoglycans, collagen and lysyl oxidase on the metabolism of low density lipoprotein by macrophages</atitle><jtitle>Atherosclerosis</jtitle><addtitle>Atherosclerosis</addtitle><date>1986-10-01</date><risdate>1986</risdate><volume>62</volume><issue>1</issue><spage>73</spage><epage>80</epage><pages>73-80</pages><issn>0021-9150</issn><eissn>1879-1484</eissn><abstract>To study the interactions of lipoproteins, connective tissue components and cells, mouse peritoneal macrophages were incubated in the presence of human low density lipoproteins (LDL) that had been complexed with pig aortic proteoglycans (PG) or incubated in the presence of soluble collagen and/or lysyl oxidase, which catalyses the formation of cross-linkages in collagen and elastin by oxidising ϵ-amino groups of lysine residues to aldehydes.
Soluble and insoluble PG-LDL complexes increased the incorporation of [
3H]oleate into cellular cholesteryl esters (CE) 1.6- and 2.8-fold, respectively, while LDL incubated with collagen and lysyl oxidase had no effect compared to control LDL. As judged on the basis of incubations with fucoidin, spermine and
125I-labelled lipoproteins, the mechanism of internalisation of the PG-LDL complexes is different from that of acetylated LDL or dextran sulphate-LDL complexes.
The formation of PG-LDL complexes in the arterial intima may lead to an increased uptake of lipoproteins by intimal macrophages during the early phase of atherogenesis.</abstract><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>2877675</pmid><doi>10.1016/0021-9150(86)90021-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Atherosclerosis, 1986-10, Vol.62 (1), p.73-80 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Oxidoreductases - metabolism Animals Aorta Atherogenesis Biological and medical sciences Cell cultures Cells, Cultured Cholesterol Esters - biosynthesis Collagen Collagen - pharmacology Disorders of blood lipids. Hyperlipoproteinemia Humans Kinetics LDL Lipoproteins, LDL - blood Lipoproteins, LDL - metabolism Lysyl oxidase Macrophages Macrophages - drug effects Macrophages - metabolism Male Medical sciences Metabolic diseases Mice Mice, Inbred Strains Protein-Lysine 6-Oxidase - metabolism Proteoglycans Proteoglycans - isolation & purification Proteoglycans - pharmacology Swine |
| title | The effect of proteoglycans, collagen and lysyl oxidase on the metabolism of low density lipoprotein by macrophages |
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