Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha

A calf thymus cDNA expression library was constructed in the Eco RI site of λgtll and probed with an antibody raised against calf thymus DNA polymerase α. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165–175 kiloda...

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Veröffentlicht in:	Biochemical and biophysical research communications 1986-10, Vol.140 (1), p.21-27
Hauptverfasser:	Foster, Kimberly, Lüthi-Steinmann, Kersten, Barnes, Marjorie, McMaster, Gary, Ferrari, Elena, Eliassen, Knut, Khan, Naseema, Brown, Neal, Hübscher, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Base Sequence beta-Galactosidase - genetics Chromatography, Affinity Cloning, Molecular DNA - analysis DNA Polymerase II - analysis DNA Polymerase II - genetics DNA Polymerase II - immunology Exact sciences and technology Other techniques and industries Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - isolation & purification
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