Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha
A calf thymus cDNA expression library was constructed in the Eco RI site of λgtll and probed with an antibody raised against calf thymus DNA polymerase α. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165–175 kiloda...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1986-10, Vol.140 (1), p.21-27 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 140 |
| creator | Foster, Kimberly Lüthi-Steinmann, Kersten Barnes, Marjorie McMaster, Gary Ferrari, Elena Eliassen, Knut Khan, Naseema Brown, Neal Hübscher, Ulrich |
| description | A calf thymus cDNA expression library was constructed in the
Eco
RI site of λgtll and probed with an antibody raised against calf thymus DNA polymerase α. Three classes of antibody-reactive clones were isolated. The largest class carried a 1.9 kilobase calf cDNA insert and expressed a 165–175 kilodalton β-galactosidase:calf fusion protein which displayed DNA polymerase activity. The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol α catalytic core. |
| doi_str_mv | 10.1016/0006-291X(86)91052-1 |
| format | Article |
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Eco
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Eco
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The characteristic responses of the polymerase activity to alpha-specific inhibitors and antibodies identified the 1.9 kilobase cDNA as a sequence specifically derived from the structural gene encoding the pol α catalytic core.</description><subject>Applied sciences</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - genetics</subject><subject>Chromatography, Affinity</subject><subject>Cloning, Molecular</subject><subject>DNA - analysis</subject><subject>DNA Polymerase II - analysis</subject><subject>DNA Polymerase II - genetics</subject><subject>DNA Polymerase II - immunology</subject><subject>Exact sciences and technology</subject><subject>Other techniques and industries</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAUxC0EKtvCNwDJB4TgELDjOLYvlaql_JEquIDEzXrrvLRGThzsbEW-PU53tUc4-TC_GfnNEPKCs3ec8fY9Y6ytasN_vtHtW8OZrCv-iGw4M6yqOWsek80JeUrOc_7FGOdNa87ImWCmFVxtyLANcfTjLYWxo_hnSpizjyONPQXqPny9oji62D0Q1MEMYZm9gxAWCm7290j7BLcDjvNqKUJP57tl2Ge6eqcYlgETZKQQpjt4Rp70EDI-P74X5MfH6-_bz9XNt09ftlc3lWu4mispm7acCKaVnXBCS2ASDaISuhV91-ldOdeYThrTKKcaXTMlsQenwEC_q8UFeX3InVL8vcc828FnhyHAiHGfrVJcilqa_4KlLsGNXBObA-hSzDlhb6fkB0iL5cyuc9i1a7t2bXVrH-awvNheHvP3uwG7k-nYf9FfHXXIa3kJRufzCdNCNkzrgl0eMCyl3XtMNjtfhsHOJ3Sz7aL_9z_-Aobwpcg</recordid><startdate>19861015</startdate><enddate>19861015</enddate><creator>Foster, Kimberly</creator><creator>Lüthi-Steinmann, Kersten</creator><creator>Barnes, Marjorie</creator><creator>McMaster, Gary</creator><creator>Ferrari, Elena</creator><creator>Eliassen, Knut</creator><creator>Khan, Naseema</creator><creator>Brown, Neal</creator><creator>Hübscher, Ulrich</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19861015</creationdate><title>Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha</title><author>Foster, Kimberly ; 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| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1986-10, Vol.140 (1), p.21-27 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77153259 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Applied sciences Base Sequence beta-Galactosidase - genetics Chromatography, Affinity Cloning, Molecular DNA - analysis DNA Polymerase II - analysis DNA Polymerase II - genetics DNA Polymerase II - immunology Exact sciences and technology Other techniques and industries Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - isolation & purification |
| title | Cloning and expression of a cDNA encoding a catalytically active fragment of calf thymus DNA polymerase alpha |
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