Classifying Acute Leukemia by Immunophenotyping: A Combined FAB-Immunologic Classification of AML
A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French-American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute...
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| Veröffentlicht in: | Blood 1986-12, Vol.68 (6), p.1355-1362 |
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| creator | Neame, Peter B. Soamboonsrup, Praniti Browman, George P. Meyer, Ralph M. Benger, Ann Wilson, W. Edwin C. Walker, Irwin R. Saeed, Niloufer McBride, John A. |
| description | A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French-American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative or inconclusive cytochemistry. At present, we suggest that until a |gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.
© 1986 by Grune & Stratton, Inc. |
| doi_str_mv | 10.1182/blood.V68.6.1355.1355 |
| format | Article |
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© 1986 by Grune & Stratton, Inc.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V68.6.1355.1355</identifier><identifier>PMID: 3465377</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Antibodies, Monoclonal ; Antigens, Neoplasm - analysis ; Antigens, Surface - analysis ; Biological and medical sciences ; Cell Differentiation ; Hematologic and hematopoietic diseases ; Humans ; Leukemia, Lymphoid - classification ; Leukemia, Lymphoid - immunology ; Leukemia, Myeloid, Acute - classification ; Leukemia, Myeloid, Acute - diagnosis ; Leukemia, Myeloid, Acute - immunology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Medical sciences ; Phenotype ; Receptors, Antigen, B-Cell - analysis</subject><ispartof>Blood, 1986-12, Vol.68 (6), p.1355-1362</ispartof><rights>1986 Copyright © 1986 The American Society of Hematology</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3515-453a28f4700c4c8998c9e61e4b4834c89fb0a707fa61f86569d49d4d665c98743</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7953854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3465377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neame, Peter B.</creatorcontrib><creatorcontrib>Soamboonsrup, Praniti</creatorcontrib><creatorcontrib>Browman, George P.</creatorcontrib><creatorcontrib>Meyer, Ralph M.</creatorcontrib><creatorcontrib>Benger, Ann</creatorcontrib><creatorcontrib>Wilson, W. Edwin C.</creatorcontrib><creatorcontrib>Walker, Irwin R.</creatorcontrib><creatorcontrib>Saeed, Niloufer</creatorcontrib><creatorcontrib>McBride, John A.</creatorcontrib><title>Classifying Acute Leukemia by Immunophenotyping: A Combined FAB-Immunologic Classification of AML</title><title>Blood</title><addtitle>Blood</addtitle><description>A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French-American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative or inconclusive cytochemistry. At present, we suggest that until a |gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.
© 1986 by Grune & Stratton, Inc.</description><subject>Antibodies, Monoclonal</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukemia, Lymphoid - classification</subject><subject>Leukemia, Lymphoid - immunology</subject><subject>Leukemia, Myeloid, Acute - classification</subject><subject>Leukemia, Myeloid, Acute - diagnosis</subject><subject>Leukemia, Myeloid, Acute - immunology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Medical sciences</subject><subject>Phenotype</subject><subject>Receptors, Antigen, B-Cell - analysis</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAQgEVo2GweP2FBh9Kbt5L1dC_BXZI0sKWXNlchy6NUjW1tLLuw_z7eXZNrYJgBzTej4UNoRcmaUp1_rZoY6_WT1Gu5pkyIYzpDSypynRGSk09oSQiRGS8UvUCXKf0jhHKWiwVaMC4FU2qJ7KaxKQW_D90zLt04AN7C-AJtsLja48e2Hbu4-wtdHPa7ifmGS7yJbRU6qPF9-T07EU18Dg7Pu4KzQ4gdjh6XP7fX6NzbJsHNXK_Qn_u735sf2fbXw-Om3GaOCSoyLpjNteeKEMedLgrtCpAUeMU1Ozz4ilhFlLeSei2FLGo-RS2lcIVWnF2hL6e9uz6-jpAG04bkoGlsB3FMRikqGOFkAsUJdH1MqQdvdn1obb83lJiDWnNUaya1RpqD1WOa5lbzB2PVQv0-Nbuc-p_nvk3ONr63nQvpHVOFYFoc7rw9YTDJ-B-gN8kF6BzUoQc3mDqGDw55A9_mlz0</recordid><startdate>198612</startdate><enddate>198612</enddate><creator>Neame, Peter B.</creator><creator>Soamboonsrup, Praniti</creator><creator>Browman, George P.</creator><creator>Meyer, Ralph M.</creator><creator>Benger, Ann</creator><creator>Wilson, W. Edwin C.</creator><creator>Walker, Irwin R.</creator><creator>Saeed, Niloufer</creator><creator>McBride, John A.</creator><general>Elsevier Inc</general><general>The Americain Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198612</creationdate><title>Classifying Acute Leukemia by Immunophenotyping: A Combined FAB-Immunologic Classification of AML</title><author>Neame, Peter B. ; Soamboonsrup, Praniti ; Browman, George P. ; Meyer, Ralph M. ; Benger, Ann ; Wilson, W. Edwin C. ; Walker, Irwin R. ; Saeed, Niloufer ; McBride, John A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3515-453a28f4700c4c8998c9e61e4b4834c89fb0a707fa61f86569d49d4d665c98743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Antibodies, Monoclonal</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukemia, Lymphoid - classification</topic><topic>Leukemia, Lymphoid - immunology</topic><topic>Leukemia, Myeloid, Acute - classification</topic><topic>Leukemia, Myeloid, Acute - diagnosis</topic><topic>Leukemia, Myeloid, Acute - immunology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Medical sciences</topic><topic>Phenotype</topic><topic>Receptors, Antigen, B-Cell - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neame, Peter B.</creatorcontrib><creatorcontrib>Soamboonsrup, Praniti</creatorcontrib><creatorcontrib>Browman, George P.</creatorcontrib><creatorcontrib>Meyer, Ralph M.</creatorcontrib><creatorcontrib>Benger, Ann</creatorcontrib><creatorcontrib>Wilson, W. Edwin C.</creatorcontrib><creatorcontrib>Walker, Irwin R.</creatorcontrib><creatorcontrib>Saeed, Niloufer</creatorcontrib><creatorcontrib>McBride, John A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neame, Peter B.</au><au>Soamboonsrup, Praniti</au><au>Browman, George P.</au><au>Meyer, Ralph M.</au><au>Benger, Ann</au><au>Wilson, W. Edwin C.</au><au>Walker, Irwin R.</au><au>Saeed, Niloufer</au><au>McBride, John A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Classifying Acute Leukemia by Immunophenotyping: A Combined FAB-Immunologic Classification of AML</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1986-12</date><risdate>1986</risdate><volume>68</volume><issue>6</issue><spage>1355</spage><epage>1362</epage><pages>1355-1362</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French-American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative or inconclusive cytochemistry. At present, we suggest that until a |gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.
© 1986 by Grune & Stratton, Inc.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>3465377</pmid><doi>10.1182/blood.V68.6.1355.1355</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antibodies, Monoclonal Antigens, Neoplasm - analysis Antigens, Surface - analysis Biological and medical sciences Cell Differentiation Hematologic and hematopoietic diseases Humans Leukemia, Lymphoid - classification Leukemia, Lymphoid - immunology Leukemia, Myeloid, Acute - classification Leukemia, Myeloid, Acute - diagnosis Leukemia, Myeloid, Acute - immunology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Phenotype Receptors, Antigen, B-Cell - analysis |
| title | Classifying Acute Leukemia by Immunophenotyping: A Combined FAB-Immunologic Classification of AML |
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