Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae
The Escherichia coli β-glucuronidase-encoding gene gusA is useful as a reporter gene in a variety of organisms. In this report, we describe the development of two related vectors, pGUS1 and pGUS2, which can be used to identify and quantitate activities of the promoter regions from yeast genes. Both...
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| Veröffentlicht in: | Gene 1995-01, Vol.154 (1), p.105-107 |
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| creator | Marathe, Sudhir V. McEwen, Joan E. |
| description | The
Escherichia coli β-glucuronidase-encoding gene
gusA is useful as a reporter gene in a variety of organisms. In this report, we describe the development of two related vectors, pGUS1 and pGUS2, which can be used to identify and quantitate activities of the promoter regions from yeast genes. Both vectors contain several unique restriction sites upstream from
gus and the yeast
CYC1 transcription terminator downstream from gus. In addition, pGUS2 carries the yeast
ADH1 transcriptional terminator sequence upstream from
gus, in order to block read-through transcription originating in vector sequences. Both vectors were tested after cloning the well-characterized
GAL1,10 promoter region from yeast. These
GAL1,10-containing plasmids demonstrated appropriate regulation of the reporter in response to carbon sources. The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA sequences. Furthermore, the presence of a terminator sequence upstream from
gus in pGUS2 should facilitate analysis and quantitation of expression from weak promoters. |
| doi_str_mv | 10.1016/0378-1119(94)00845-J |
| format | Article |
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Escherichia coli β-glucuronidase-encoding gene
gusA is useful as a reporter gene in a variety of organisms. In this report, we describe the development of two related vectors, pGUS1 and pGUS2, which can be used to identify and quantitate activities of the promoter regions from yeast genes. Both vectors contain several unique restriction sites upstream from
gus and the yeast
CYC1 transcription terminator downstream from gus. In addition, pGUS2 carries the yeast
ADH1 transcriptional terminator sequence upstream from
gus, in order to block read-through transcription originating in vector sequences. Both vectors were tested after cloning the well-characterized
GAL1,10 promoter region from yeast. These
GAL1,10-containing plasmids demonstrated appropriate regulation of the reporter in response to carbon sources. The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA sequences. Furthermore, the presence of a terminator sequence upstream from
gus in pGUS2 should facilitate analysis and quantitation of expression from weak promoters.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)00845-J</identifier><identifier>PMID: 7867935</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alcohol Dehydrogenase - genetics ; Bacterial Proteins - genetics ; Base Sequence ; beta-glucuronidase ; Cytochrome c Group - genetics ; Cytochromes c ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli gus A ; Fungal Proteins - genetics ; GAL1,10 promoter ; Gene Expression Regulation, Fungal ; Genes, Bacterial ; Genes, Reporter ; genetic techniques and protocols ; Genetic Vectors - genetics ; Glucuronidase - genetics ; gusa gene ; Molecular Sequence Data ; plasmid vectors ; promoter regions ; Promoter Regions, Genetic ; recombinant DNA ; Recombinant Fusion Proteins - genetics ; reporter genes ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Terminator Regions, Genetic ; transcription ; yeast ; β-glucuronidase</subject><ispartof>Gene, 1995-01, Vol.154 (1), p.105-107</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-143e93a31bbc342564112a8bd54679809ff39a57eb1c2861910d7c6cecff8a3f3</citedby><cites>FETCH-LOGICAL-c412t-143e93a31bbc342564112a8bd54679809ff39a57eb1c2861910d7c6cecff8a3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)00845-J$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7867935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marathe, Sudhir V.</creatorcontrib><creatorcontrib>McEwen, Joan E.</creatorcontrib><title>Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae</title><title>Gene</title><addtitle>Gene</addtitle><description>The
Escherichia coli β-glucuronidase-encoding gene
gusA is useful as a reporter gene in a variety of organisms. In this report, we describe the development of two related vectors, pGUS1 and pGUS2, which can be used to identify and quantitate activities of the promoter regions from yeast genes. Both vectors contain several unique restriction sites upstream from
gus and the yeast
CYC1 transcription terminator downstream from gus. In addition, pGUS2 carries the yeast
ADH1 transcriptional terminator sequence upstream from
gus, in order to block read-through transcription originating in vector sequences. Both vectors were tested after cloning the well-characterized
GAL1,10 promoter region from yeast. These
GAL1,10-containing plasmids demonstrated appropriate regulation of the reporter in response to carbon sources. The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA sequences. Furthermore, the presence of a terminator sequence upstream from
gus in pGUS2 should facilitate analysis and quantitation of expression from weak promoters.</description><subject>Alcohol Dehydrogenase - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>beta-glucuronidase</subject><subject>Cytochrome c Group - genetics</subject><subject>Cytochromes c</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli gus A</subject><subject>Fungal Proteins - genetics</subject><subject>GAL1,10 promoter</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Bacterial</subject><subject>Genes, Reporter</subject><subject>genetic techniques and protocols</subject><subject>Genetic Vectors - genetics</subject><subject>Glucuronidase - genetics</subject><subject>gusa gene</subject><subject>Molecular Sequence Data</subject><subject>plasmid vectors</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant DNA</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>reporter genes</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Terminator Regions, Genetic</subject><subject>transcription</subject><subject>yeast</subject><subject>β-glucuronidase</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkPhH4DwCsEi4BvbSbyphCoerSqxKGVrOc51xmgmntpO0fz7Osyoy9Ybyz7ffegcQt4C-wwMmi-Mt10FAOqjEp8Y64SsLp-RFXStqhjj3XOyekBeklcp_WXlSFmfkJO2a1rF5Yrs_qDNISb6z-c1zWuk45xoxF2IGSMdcULqQqR-wCl7t_fTSM000NvZlHc2efnYxbANCx5x9GFK1E_02li7NkXYW0zUYsQ7n7zB1-SFM5uEb473Kbn5_u33-c_q6tePi_OvV5UVUOcKBEfFDYe-t1zUshEAten6QYqyeceUc1wZ2WIPtu4aUMCG1jYWrXOd4Y6fkg-HvmW52xlT1lufLG42ZsIwJ922IIEpeBKEMq9hghVQHEAbQ0oRnd5FvzVxr4HpJRG92K0Xu7US-n8i-rKUvTv2n_stDg9FxwiK_v6gOxO0GaNP-ua6ZsAZSAaKLcTZgcDi153HqJP1OFkcfCzp6SH4x1e4B2NzpYc</recordid><startdate>19950101</startdate><enddate>19950101</enddate><creator>Marathe, Sudhir V.</creator><creator>McEwen, Joan E.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19950101</creationdate><title>Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae</title><author>Marathe, Sudhir V. ; McEwen, Joan E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-143e93a31bbc342564112a8bd54679809ff39a57eb1c2861910d7c6cecff8a3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alcohol Dehydrogenase - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>beta-glucuronidase</topic><topic>Cytochrome c Group - genetics</topic><topic>Cytochromes c</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli gus A</topic><topic>Fungal Proteins - genetics</topic><topic>GAL1,10 promoter</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Bacterial</topic><topic>Genes, Reporter</topic><topic>genetic techniques and protocols</topic><topic>Genetic Vectors - genetics</topic><topic>Glucuronidase - genetics</topic><topic>gusa gene</topic><topic>Molecular Sequence Data</topic><topic>plasmid vectors</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant DNA</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>reporter genes</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Terminator Regions, Genetic</topic><topic>transcription</topic><topic>yeast</topic><topic>β-glucuronidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marathe, Sudhir V.</creatorcontrib><creatorcontrib>McEwen, Joan E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marathe, Sudhir V.</au><au>McEwen, Joan E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1995-01-01</date><risdate>1995</risdate><volume>154</volume><issue>1</issue><spage>105</spage><epage>107</epage><pages>105-107</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The
Escherichia coli β-glucuronidase-encoding gene
gusA is useful as a reporter gene in a variety of organisms. In this report, we describe the development of two related vectors, pGUS1 and pGUS2, which can be used to identify and quantitate activities of the promoter regions from yeast genes. Both vectors contain several unique restriction sites upstream from
gus and the yeast
CYC1 transcription terminator downstream from gus. In addition, pGUS2 carries the yeast
ADH1 transcriptional terminator sequence upstream from
gus, in order to block read-through transcription originating in vector sequences. Both vectors were tested after cloning the well-characterized
GAL1,10 promoter region from yeast. These
GAL1,10-containing plasmids demonstrated appropriate regulation of the reporter in response to carbon sources. The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA sequences. Furthermore, the presence of a terminator sequence upstream from
gus in pGUS2 should facilitate analysis and quantitation of expression from weak promoters.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7867935</pmid><doi>10.1016/0378-1119(94)00845-J</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1995-01, Vol.154 (1), p.105-107 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alcohol Dehydrogenase - genetics Bacterial Proteins - genetics Base Sequence beta-glucuronidase Cytochrome c Group - genetics Cytochromes c Escherichia coli Escherichia coli - genetics Escherichia coli gus A Fungal Proteins - genetics GAL1,10 promoter Gene Expression Regulation, Fungal Genes, Bacterial Genes, Reporter genetic techniques and protocols Genetic Vectors - genetics Glucuronidase - genetics gusa gene Molecular Sequence Data plasmid vectors promoter regions Promoter Regions, Genetic recombinant DNA Recombinant Fusion Proteins - genetics reporter genes Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Terminator Regions, Genetic transcription yeast β-glucuronidase |
| title | Vectors with the gus reporter gene for identifying and quantitating promoter regions in Saccharomyces cerevisiae |
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