Ultrastructure of the 5‐Hydroxytryptamine3 Receptor
: We have determined the ultrastructure of 5‐hydroxytryptamine3 (5‐HT3) serotonin receptors purified from NG108‐15 mouse neuroblastoma × rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors. The 5‐H...
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| Veröffentlicht in: | Journal of neurochemistry 1995-03, Vol.64 (3), p.1401-1405 |
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| container_title | Journal of neurochemistry |
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| creator | Boess, Frank G. Beroukhim, Rameen Martin, Ian L. |
| description | : We have determined the ultrastructure of 5‐hydroxytryptamine3 (5‐HT3) serotonin receptors purified from NG108‐15 mouse neuroblastoma × rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors. The 5‐HT3 receptor can be modelled as a cylinder 11 nm in length and 8 nm in diameter with a closed end and a central cavity 3 nm in diameter. Analysis of the rotational symmetry of single receptor particles indicates that the 5‐HT3 receptor is composed of five subunits arranged symmetrically around a central cavity. Together with evidence obtained for related proteins in other studies using ultrastructural, biochemical, or electrophysiological methods, our observations suggest that all members of the ligand‐gated ion channel superfamily may possess a pentameric quaternary structure. |
| doi_str_mv | 10.1046/j.1471-4159.1995.64031401.x |
| format | Article |
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Psychology ; Hybrid Cells ; Ion Channel Gating ; Mice ; Microscopy, Electron ; Molecular and cellular biology ; Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) ; Protein Conformation ; Quaternary structure ; Rats ; Receptors, Serotonin - ultrastructure ; Rotational symmetry</subject><ispartof>Journal of neurochemistry, 1995-03, Vol.64 (3), p.1401-1405</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.1995.64031401.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.1995.64031401.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3452413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7861173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boess, Frank G.</creatorcontrib><creatorcontrib>Beroukhim, Rameen</creatorcontrib><creatorcontrib>Martin, Ian L.</creatorcontrib><title>Ultrastructure of the 5‐Hydroxytryptamine3 Receptor</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: We have determined the ultrastructure of 5‐hydroxytryptamine3 (5‐HT3) serotonin receptors purified from NG108‐15 mouse neuroblastoma × rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors. The 5‐HT3 receptor can be modelled as a cylinder 11 nm in length and 8 nm in diameter with a closed end and a central cavity 3 nm in diameter. Analysis of the rotational symmetry of single receptor particles indicates that the 5‐HT3 receptor is composed of five subunits arranged symmetrically around a central cavity. Together with evidence obtained for related proteins in other studies using ultrastructural, biochemical, or electrophysiological methods, our observations suggest that all members of the ligand‐gated ion channel superfamily may possess a pentameric quaternary structure.</description><subject>5‐Hydroxytryptamine3 receptor</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Electron microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hybrid Cells</subject><subject>Ion Channel Gating</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Molecular and cellular biology</subject><subject>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</subject><subject>Protein Conformation</subject><subject>Quaternary structure</subject><subject>Rats</subject><subject>Receptors, Serotonin - ultrastructure</subject><subject>Rotational symmetry</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kN1KwzAUx4Moc04fQSgo3rXmNEmzXMr8mDIUxF2HNE2xpV1r0uJ65yP4jD6JLet2dTj8f5yPH0JXgAPANLrNA6AcfApMBCAECyKKCVAMwfYITQ_ZMZpiHIY-wTQ8RWfO5RhDRCOYoAmfRwCcTBFbF41VrrGtblprvCr1mk_jsb-f32WX2GrbNbarG1VmG0O8d6NN3VT2HJ2kqnDmYqwztH58-Fgs_dXb0_PibuXnIe-vYBonOMWJpgoLw3XECFOKR1owJrghXIHAoQauSUyJIJDqJI3nXKkQK85iMkM3u7m1rb5a4xpZZk6bolAbU7VO9kuo4FT04OUItnFpElnbrFS2k-OffX495sppVaRWbXTmDhihLKQwYPc77DsrTHeIActBvMzlIFcOcuUgXu7Fy618eV3sO_IPsaF3rA</recordid><startdate>199503</startdate><enddate>199503</enddate><creator>Boess, Frank G.</creator><creator>Beroukhim, Rameen</creator><creator>Martin, Ian L.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199503</creationdate><title>Ultrastructure of the 5‐Hydroxytryptamine3 Receptor</title><author>Boess, Frank G. ; Beroukhim, Rameen ; Martin, Ian L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j2771-5c0d0f0dc4a09e7c6535aa76c95597e37a1902c17c3b43931fcdfb87aa20a75b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>5‐Hydroxytryptamine3 receptor</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Electron microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hybrid Cells</topic><topic>Ion Channel Gating</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Molecular and cellular biology</topic><topic>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</topic><topic>Protein Conformation</topic><topic>Quaternary structure</topic><topic>Rats</topic><topic>Receptors, Serotonin - ultrastructure</topic><topic>Rotational symmetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boess, Frank G.</creatorcontrib><creatorcontrib>Beroukhim, Rameen</creatorcontrib><creatorcontrib>Martin, Ian L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boess, Frank G.</au><au>Beroukhim, Rameen</au><au>Martin, Ian L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructure of the 5‐Hydroxytryptamine3 Receptor</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1995-03</date><risdate>1995</risdate><volume>64</volume><issue>3</issue><spage>1401</spage><epage>1405</epage><pages>1401-1405</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: We have determined the ultrastructure of 5‐hydroxytryptamine3 (5‐HT3) serotonin receptors purified from NG108‐15 mouse neuroblastoma × rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors. The 5‐HT3 receptor can be modelled as a cylinder 11 nm in length and 8 nm in diameter with a closed end and a central cavity 3 nm in diameter. Analysis of the rotational symmetry of single receptor particles indicates that the 5‐HT3 receptor is composed of five subunits arranged symmetrically around a central cavity. Together with evidence obtained for related proteins in other studies using ultrastructural, biochemical, or electrophysiological methods, our observations suggest that all members of the ligand‐gated ion channel superfamily may possess a pentameric quaternary structure.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>7861173</pmid><doi>10.1046/j.1471-4159.1995.64031401.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1995-03, Vol.64 (3), p.1401-1405 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 5‐Hydroxytryptamine3 receptor Animals Biological and medical sciences Cell receptors Cell structures and functions Electron microscopy Fundamental and applied biological sciences. Psychology Hybrid Cells Ion Channel Gating Mice Microscopy, Electron Molecular and cellular biology Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Protein Conformation Quaternary structure Rats Receptors, Serotonin - ultrastructure Rotational symmetry |
| title | Ultrastructure of the 5‐Hydroxytryptamine3 Receptor |
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