Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line
We succeeded in establishing a human gastric carcinoma cell line (KE-97) from oncocytes obtained from the mesentery disseminated metastatic focus of a 52-year-old male stomach cancer patient. From a histopathological point of view the gastric carcinoma was clearly a mucin-producing mucinous carcinom...
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| Veröffentlicht in: | Nippon Shokakibyo Gakkai Zasshi 1995, Vol.92(1), pp.19-25 |
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| container_title | Nippon Shokakibyo Gakkai Zasshi |
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| creator | UESUGI, Hidenaga ATARI, Eio |
| description | We succeeded in establishing a human gastric carcinoma cell line (KE-97) from oncocytes obtained from the mesentery disseminated metastatic focus of a 52-year-old male stomach cancer patient. From a histopathological point of view the gastric carcinoma was clearly a mucin-producing mucinous carcinoma, portion of which were mixed with poorly differentiated adenocarcinoma and signet-ring cell carcinoma. The oncocytes were fused into a mass and exhibited a suspended proliferating system, with a doubling time of about 28.8 hours. Transplantation of the cancer cells into skid mice resulted in a tumor system in all cases, and histologically, mucinous vacuoles were found in the cell membranes. With immunological staining they were found to be positive for anti-CEA antibodies, anti-CA19-9 antibodies and anti-ICAM-1 antibodies. Autopsy found extensive hematogenous metastasis (lung, liver) and cancerous peritonitis. KE-97 is mucinous carcinoma, and it was reported with the belief that it is a useful cell line upon the investigation of its cancer metastasis mechanism and cytological characteristics. |
| doi_str_mv | 10.11405/nisshoshi1964.92.19 |
| format | Article |
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From a histopathological point of view the gastric carcinoma was clearly a mucin-producing mucinous carcinoma, portion of which were mixed with poorly differentiated adenocarcinoma and signet-ring cell carcinoma. The oncocytes were fused into a mass and exhibited a suspended proliferating system, with a doubling time of about 28.8 hours. Transplantation of the cancer cells into skid mice resulted in a tumor system in all cases, and histologically, mucinous vacuoles were found in the cell membranes. With immunological staining they were found to be positive for anti-CEA antibodies, anti-CA19-9 antibodies and anti-ICAM-1 antibodies. Autopsy found extensive hematogenous metastasis (lung, liver) and cancerous peritonitis. KE-97 is mucinous carcinoma, and it was reported with the belief that it is a useful cell line upon the investigation of its cancer metastasis mechanism and cytological characteristics.</description><identifier>ISSN: 0446-6586</identifier><identifier>EISSN: 1349-7693</identifier><identifier>DOI: 10.11405/nisshoshi1964.92.19</identifier><identifier>PMID: 7861622</identifier><language>jpn</language><publisher>Japan: The Japanese Society of Gastroenterology</publisher><subject>Adenocarcinoma - pathology ; Adenocarcinoma, Mucinous - pathology ; Animals ; Carcinoma, Signet Ring Cell - pathology ; Humans ; Male ; Mice ; Mice, Nude ; Middle Aged ; Neoplasm Transplantation ; Stomach Neoplasms - pathology ; Tumor Cells, Cultured - pathology</subject><ispartof>Nippon Shokakibyo Gakkai Zasshi, 1995, Vol.92(1), pp.19-25</ispartof><rights>The Japanese Society of Gastroenterology</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7861622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>UESUGI, Hidenaga</creatorcontrib><creatorcontrib>ATARI, Eio</creatorcontrib><title>Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line</title><title>Nippon Shokakibyo Gakkai Zasshi</title><addtitle>Nippon Shokakibyo Gakkai Zasshi</addtitle><description>We succeeded in establishing a human gastric carcinoma cell line (KE-97) from oncocytes obtained from the mesentery disseminated metastatic focus of a 52-year-old male stomach cancer patient. 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KE-97 is mucinous carcinoma, and it was reported with the belief that it is a useful cell line upon the investigation of its cancer metastasis mechanism and cytological characteristics.</description><subject>Adenocarcinoma - pathology</subject><subject>Adenocarcinoma, Mucinous - pathology</subject><subject>Animals</subject><subject>Carcinoma, Signet Ring Cell - pathology</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Neoplasm Transplantation</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tumor Cells, Cultured - pathology</subject><issn>0446-6586</issn><issn>1349-7693</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE9LJDEQxYPsooP6DVbIaW89m3-dTo6LuLogeNFzU52uno6k02OSRubb2zKD4KXq8H5VvPcI-cXZlnPF6j_R5zzOefTcarW1YsvtGdlwqWzVaCt_kA1TSle6NvqCXOfsO8aYra2R8pycN0ZzLcSG4F0u0AWfxwljoRB7uocyzmHeeQeB5rL0BzoPFGjEd7qf5xQOtPfDgGk98FCwp9PifJyXTHeQS_KOOogOE3UYAg0-4hX5OUDIeH3al-Tl393z7UP1-HT___bvY_UqlCyV6ZiqJdSOGbemstyIuhbMaIsWGRO8H5gWwFTHwWmjLAPZdQ0bGiYVcCsvye_j332a3xbMpZ18_nQBEVd_bdNwZWtlVvDmBC7dhH27T36CdGhPvaz6w1F_XevZ4ZcOqXgXsP3WfmtFy4-D2y_EjZBajPIDS-uC0w</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>UESUGI, Hidenaga</creator><creator>ATARI, Eio</creator><general>The Japanese Society of Gastroenterology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line</title><author>UESUGI, Hidenaga ; ATARI, Eio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j243t-8b0453a5c08c96491825520869e9e0021df062a04b1ac68490a3bb70f7034a193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>1995</creationdate><topic>Adenocarcinoma - pathology</topic><topic>Adenocarcinoma, Mucinous - pathology</topic><topic>Animals</topic><topic>Carcinoma, Signet Ring Cell - pathology</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Neoplasm Transplantation</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tumor Cells, Cultured - pathology</topic><toplevel>online_resources</toplevel><creatorcontrib>UESUGI, Hidenaga</creatorcontrib><creatorcontrib>ATARI, Eio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nippon Shokakibyo Gakkai Zasshi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UESUGI, Hidenaga</au><au>ATARI, Eio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line</atitle><jtitle>Nippon Shokakibyo Gakkai Zasshi</jtitle><addtitle>Nippon Shokakibyo Gakkai Zasshi</addtitle><date>1995</date><risdate>1995</risdate><volume>92</volume><issue>1</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>0446-6586</issn><eissn>1349-7693</eissn><abstract>We succeeded in establishing a human gastric carcinoma cell line (KE-97) from oncocytes obtained from the mesentery disseminated metastatic focus of a 52-year-old male stomach cancer patient. From a histopathological point of view the gastric carcinoma was clearly a mucin-producing mucinous carcinoma, portion of which were mixed with poorly differentiated adenocarcinoma and signet-ring cell carcinoma. The oncocytes were fused into a mass and exhibited a suspended proliferating system, with a doubling time of about 28.8 hours. Transplantation of the cancer cells into skid mice resulted in a tumor system in all cases, and histologically, mucinous vacuoles were found in the cell membranes. With immunological staining they were found to be positive for anti-CEA antibodies, anti-CA19-9 antibodies and anti-ICAM-1 antibodies. Autopsy found extensive hematogenous metastasis (lung, liver) and cancerous peritonitis. KE-97 is mucinous carcinoma, and it was reported with the belief that it is a useful cell line upon the investigation of its cancer metastasis mechanism and cytological characteristics.</abstract><cop>Japan</cop><pub>The Japanese Society of Gastroenterology</pub><pmid>7861622</pmid><doi>10.11405/nisshoshi1964.92.19</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0446-6586 |
| ispartof | Nippon Shokakibyo Gakkai Zasshi, 1995, Vol.92(1), pp.19-25 |
| issn | 0446-6586 1349-7693 |
| language | jpn |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenocarcinoma - pathology Adenocarcinoma, Mucinous - pathology Animals Carcinoma, Signet Ring Cell - pathology Humans Male Mice Mice, Nude Middle Aged Neoplasm Transplantation Stomach Neoplasms - pathology Tumor Cells, Cultured - pathology |
| title | Establishment and pathological study of a new poorly differentiated mucinous gastric cancer cell line |
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