Development and characterization of essential fatty acid deficiency in human endothelial cells in culture

We induced an essential fatty acid deficiency (EFAD) in human umbilical vein endothelial cells by culture in medium with 20% (vol/vol) delipidated fetal calf serum. EFAD, reflected by decreased cellular linoleic acid (18:2 omega 6) and arachidonic acid (20:4 omega 6) and emergence of the oleic acid...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1995-02, Vol.92 (4), p.1147-1151
Hauptverfasser:	Lerner, Richard, Lindstrom, Peter, Berg, Anders, Johansson, Elsbeth, Rosendahl, Kerstin, Palmblad, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	acide arachidonique acide gras essentiel acide gras insature acide gras polyinsature acide linoleique acido araquidonico acido linoleico acidos grasos esenciales acidos grasos insaturados acidos grasos poliinsaturados arachidonic acid blood veins Calcium - metabolism cell culture Cell Division Cell growth Cells Cells, Cultured Cellular biology chemical composition composicion quimica composition chimique corps gras cultivo de celulas culture de cellule culture media Cultured cells deficiency diseases Eicosanoids Endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - metabolism enfermedades carenciales epitelio epithelium Epoprostenol - metabolism Essential fatty acids fats Fatty acids Fatty Acids, Essential - deficiency Fatty Acids, Essential - metabolism genero humano genre humain grasas Human umbilical vein endothelial cells Humans linoleic acid Lipid Metabolism, Inborn Errors - metabolism Lipids maladie de carence mankind medio de cultivo milieu de culture Nonesterified fatty acids polyunsaturated fatty acids Prostaglandins unsaturated fatty acids veine venas
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