Effects of zinc deficiency on protein synthesis and expression of specific mRNAs in rat liver
The effects of zinc deficiency on protein synthesis and expression of specific mRNAs were assessed in rat liver. Zinc deficiency had no apparent effect on liver weight, protein content, or RNA content when these properties were compared with values obtained using pair-fed rats. However, zinc deficie...
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| Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 1995, Vol.44 (1), p.126-133 |
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| creator | Kimball, Scot R. Chen, Sei-Jei Risica, Robert Jefferson, Leonard S. Leure-dupree, Alphonse E. |
| description | The effects of zinc deficiency on protein synthesis and expression of specific mRNAs were assessed in rat liver. Zinc deficiency had no apparent effect on liver weight, protein content, or RNA content when these properties were compared with values obtained using pair-fed rats. However, zinc deficiency resulted in a lower rate of hepatic protein synthesis. The decreased rate of protein synthesis was due to a decrease in the rate of synthesis of proteins retained in the liver, with no apparent change in the synthesis of secreted proteins. Analysis of expression of specific gene products, as assessed by in vitro translation of total RNA followed by two.dimensional gel analysis, showed that the expression of only a few mRNAs was altered by zinc deficiency. The patterns of change in gene expression resulting from zinc deficiency varied from almost complete repression to full expression. In additional studies, cDNA clones to serum retinol-binding protein and transthyretin were used to examine the effect of zinc deficiency on the relative abundance of mRNA for these two proteins. The relative abundance of mRNA for transthyretin was specifically elevated as a result of zinc deficiency. In contrast, the relative abundance of mRNA for hepatic serum retinol-binding protein was increased in both zinc-deficient and pair-fed rats. Therefore, the observed change in mRNA for serum retinol-binding protein was apparently at least in part due to the inanition that accompanies zinc deficiency. Overall, the results suggest that zinc can regulate the synthesis of specific proteins in rat liver through changes in the relative abundance of specific mRNAs. |
| doi_str_mv | 10.1016/0026-0495(95)90299-6 |
| format | Article |
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Zinc deficiency had no apparent effect on liver weight, protein content, or RNA content when these properties were compared with values obtained using pair-fed rats. However, zinc deficiency resulted in a lower rate of hepatic protein synthesis. The decreased rate of protein synthesis was due to a decrease in the rate of synthesis of proteins retained in the liver, with no apparent change in the synthesis of secreted proteins. Analysis of expression of specific gene products, as assessed by in vitro translation of total RNA followed by two.dimensional gel analysis, showed that the expression of only a few mRNAs was altered by zinc deficiency. The patterns of change in gene expression resulting from zinc deficiency varied from almost complete repression to full expression. In additional studies, cDNA clones to serum retinol-binding protein and transthyretin were used to examine the effect of zinc deficiency on the relative abundance of mRNA for these two proteins. The relative abundance of mRNA for transthyretin was specifically elevated as a result of zinc deficiency. In contrast, the relative abundance of mRNA for hepatic serum retinol-binding protein was increased in both zinc-deficient and pair-fed rats. Therefore, the observed change in mRNA for serum retinol-binding protein was apparently at least in part due to the inanition that accompanies zinc deficiency. Overall, the results suggest that zinc can regulate the synthesis of specific proteins in rat liver through changes in the relative abundance of specific mRNAs.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/0026-0495(95)90299-6</identifier><identifier>PMID: 7854157</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>ADN ; ANIMALES JOVENES ; Animals ; ARN ; ARN MENSAJERO ; ARN MESSAGER ; BINDING PROTEINS ; Biological and medical sciences ; BLOOD PROTEINS ; CARENCE EN OLIGOELEMENT ; CINC ; CLONE ; CLONES ; COMPLEMENTARY DNA ; DEFICIENCIA DE OLIGOELEMENTOS ; DNA ; Electrophoresis, Gel, Two-Dimensional ; EXPRESION GENICA ; EXPRESSION DES GENES ; FOIE ; GENE EXPRESSION ; HIGADO ; In Vitro Techniques ; JEUNE ANIMAL ; LIVER ; Liver - metabolism ; Male ; Medical sciences ; MESSENGER RNA ; Metabolic diseases ; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) ; Prealbumin - genetics ; Protein Biosynthesis ; PROTEIN SYNTHESIS ; PROTEINAS AGLUTINANTES ; PROTEINAS SANGUINEAS ; PROTEINE DE LIAISON ; PROTEINE SANGUINE ; RAT ; RATA ; RATS ; Rats, Inbred Strains ; Retinol-Binding Proteins - genetics ; RNA ; RNA, Messenger - metabolism ; serum retinol-binding protein ; SINTESIS DE PROTEINAS ; SYNTHESE PROTEIQUE ; TRACE ELEMENT DEFICIENCIES ; TRANSTHYRETIN ; weanling rats ; YOUNG ANIMALS ; ZINC ; Zinc - deficiency</subject><ispartof>Metabolism, clinical and experimental, 1995, Vol.44 (1), p.126-133</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-13fc8c980ff53318b32db8c49d13e95cb72a9e951dc04a86789f63a17f1d2f2a3</citedby><cites>FETCH-LOGICAL-c386t-13fc8c980ff53318b32db8c49d13e95cb72a9e951dc04a86789f63a17f1d2f2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0026-0495(95)90299-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3392367$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7854157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kimball, Scot R.</creatorcontrib><creatorcontrib>Chen, Sei-Jei</creatorcontrib><creatorcontrib>Risica, Robert</creatorcontrib><creatorcontrib>Jefferson, Leonard S.</creatorcontrib><creatorcontrib>Leure-dupree, Alphonse E.</creatorcontrib><title>Effects of zinc deficiency on protein synthesis and expression of specific mRNAs in rat liver</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>The effects of zinc deficiency on protein synthesis and expression of specific mRNAs were assessed in rat liver. Zinc deficiency had no apparent effect on liver weight, protein content, or RNA content when these properties were compared with values obtained using pair-fed rats. However, zinc deficiency resulted in a lower rate of hepatic protein synthesis. The decreased rate of protein synthesis was due to a decrease in the rate of synthesis of proteins retained in the liver, with no apparent change in the synthesis of secreted proteins. Analysis of expression of specific gene products, as assessed by in vitro translation of total RNA followed by two.dimensional gel analysis, showed that the expression of only a few mRNAs was altered by zinc deficiency. The patterns of change in gene expression resulting from zinc deficiency varied from almost complete repression to full expression. In additional studies, cDNA clones to serum retinol-binding protein and transthyretin were used to examine the effect of zinc deficiency on the relative abundance of mRNA for these two proteins. The relative abundance of mRNA for transthyretin was specifically elevated as a result of zinc deficiency. In contrast, the relative abundance of mRNA for hepatic serum retinol-binding protein was increased in both zinc-deficient and pair-fed rats. Therefore, the observed change in mRNA for serum retinol-binding protein was apparently at least in part due to the inanition that accompanies zinc deficiency. Overall, the results suggest that zinc can regulate the synthesis of specific proteins in rat liver through changes in the relative abundance of specific mRNAs.</description><subject>ADN</subject><subject>ANIMALES JOVENES</subject><subject>Animals</subject><subject>ARN</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>BINDING PROTEINS</subject><subject>Biological and medical sciences</subject><subject>BLOOD PROTEINS</subject><subject>CARENCE EN OLIGOELEMENT</subject><subject>CINC</subject><subject>CLONE</subject><subject>CLONES</subject><subject>COMPLEMENTARY DNA</subject><subject>DEFICIENCIA DE OLIGOELEMENTOS</subject><subject>DNA</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FOIE</subject><subject>GENE EXPRESSION</subject><subject>HIGADO</subject><subject>In Vitro Techniques</subject><subject>JEUNE ANIMAL</subject><subject>LIVER</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>MESSENGER RNA</subject><subject>Metabolic diseases</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>Prealbumin - genetics</subject><subject>Protein Biosynthesis</subject><subject>PROTEIN SYNTHESIS</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINAS SANGUINEAS</subject><subject>PROTEINE DE LIAISON</subject><subject>PROTEINE SANGUINE</subject><subject>RAT</subject><subject>RATA</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>Retinol-Binding Proteins - genetics</subject><subject>RNA</subject><subject>RNA, Messenger - metabolism</subject><subject>serum retinol-binding protein</subject><subject>SINTESIS DE PROTEINAS</subject><subject>SYNTHESE PROTEIQUE</subject><subject>TRACE ELEMENT DEFICIENCIES</subject><subject>TRANSTHYRETIN</subject><subject>weanling rats</subject><subject>YOUNG ANIMALS</subject><subject>ZINC</subject><subject>Zinc - deficiency</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9rFDEUxYNY6tr6BUQhD0Xqw2gymfx7EUqpVSgK1T5KyCY3GpmdWXNnS9dPb8Zd9rEQSOD8zsnlXEJecfaOM67eM9aqhnVWnlv51rLW2kY9IQsuRdsYxdhTsjggz8hzxN-MMa2NOibH2siOS70gP65SgjAhHRP9m4dAI6QcMgxhS8eBrss4QR4obofpF2BG6odI4WFdADFXoNpwDSFXE13dfrlAWuniJ9rneyin5Cj5HuHF_j4hdx-vvl9-am6-Xn--vLhpgjBqarhIwQRrWEpSCG6Woo1LEzobuQArw1K33tYHj4F13ihtbFLCc514bFPrxQl5s8ut8_7ZAE5ulTFA3_sBxg06rXknDdMV7HZgKCNigeTWJa982TrO3Nyqmytzc2VuPnOrTlXb633-ZrmCeDDta6z62V73GHyfih9CxgMmhG2FmrGXOyz50fmfpSJ336wUNWT-48NOhFrUfYbi8P8eIOZSN-TimB8f8h-pWpuA</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Kimball, Scot R.</creator><creator>Chen, Sei-Jei</creator><creator>Risica, Robert</creator><creator>Jefferson, Leonard S.</creator><creator>Leure-dupree, Alphonse E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Effects of zinc deficiency on protein synthesis and expression of specific mRNAs in rat liver</title><author>Kimball, Scot R. ; Chen, Sei-Jei ; Risica, Robert ; Jefferson, Leonard S. ; Leure-dupree, Alphonse E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-13fc8c980ff53318b32db8c49d13e95cb72a9e951dc04a86789f63a17f1d2f2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ADN</topic><topic>ANIMALES JOVENES</topic><topic>Animals</topic><topic>ARN</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>BINDING PROTEINS</topic><topic>Biological and medical sciences</topic><topic>BLOOD PROTEINS</topic><topic>CARENCE EN OLIGOELEMENT</topic><topic>CINC</topic><topic>CLONE</topic><topic>CLONES</topic><topic>COMPLEMENTARY DNA</topic><topic>DEFICIENCIA DE OLIGOELEMENTOS</topic><topic>DNA</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FOIE</topic><topic>GENE EXPRESSION</topic><topic>HIGADO</topic><topic>In Vitro Techniques</topic><topic>JEUNE ANIMAL</topic><topic>LIVER</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>MESSENGER RNA</topic><topic>Metabolic diseases</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>Prealbumin - genetics</topic><topic>Protein Biosynthesis</topic><topic>PROTEIN SYNTHESIS</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINAS SANGUINEAS</topic><topic>PROTEINE DE LIAISON</topic><topic>PROTEINE SANGUINE</topic><topic>RAT</topic><topic>RATA</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>Retinol-Binding Proteins - genetics</topic><topic>RNA</topic><topic>RNA, Messenger - metabolism</topic><topic>serum retinol-binding protein</topic><topic>SINTESIS DE PROTEINAS</topic><topic>SYNTHESE PROTEIQUE</topic><topic>TRACE ELEMENT DEFICIENCIES</topic><topic>TRANSTHYRETIN</topic><topic>weanling rats</topic><topic>YOUNG ANIMALS</topic><topic>ZINC</topic><topic>Zinc - deficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimball, Scot R.</creatorcontrib><creatorcontrib>Chen, Sei-Jei</creatorcontrib><creatorcontrib>Risica, Robert</creatorcontrib><creatorcontrib>Jefferson, Leonard S.</creatorcontrib><creatorcontrib>Leure-dupree, Alphonse E.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimball, Scot R.</au><au>Chen, Sei-Jei</au><au>Risica, Robert</au><au>Jefferson, Leonard S.</au><au>Leure-dupree, Alphonse E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of zinc deficiency on protein synthesis and expression of specific mRNAs in rat liver</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>1995</date><risdate>1995</risdate><volume>44</volume><issue>1</issue><spage>126</spage><epage>133</epage><pages>126-133</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>The effects of zinc deficiency on protein synthesis and expression of specific mRNAs were assessed in rat liver. Zinc deficiency had no apparent effect on liver weight, protein content, or RNA content when these properties were compared with values obtained using pair-fed rats. However, zinc deficiency resulted in a lower rate of hepatic protein synthesis. The decreased rate of protein synthesis was due to a decrease in the rate of synthesis of proteins retained in the liver, with no apparent change in the synthesis of secreted proteins. Analysis of expression of specific gene products, as assessed by in vitro translation of total RNA followed by two.dimensional gel analysis, showed that the expression of only a few mRNAs was altered by zinc deficiency. The patterns of change in gene expression resulting from zinc deficiency varied from almost complete repression to full expression. In additional studies, cDNA clones to serum retinol-binding protein and transthyretin were used to examine the effect of zinc deficiency on the relative abundance of mRNA for these two proteins. The relative abundance of mRNA for transthyretin was specifically elevated as a result of zinc deficiency. In contrast, the relative abundance of mRNA for hepatic serum retinol-binding protein was increased in both zinc-deficient and pair-fed rats. Therefore, the observed change in mRNA for serum retinol-binding protein was apparently at least in part due to the inanition that accompanies zinc deficiency. Overall, the results suggest that zinc can regulate the synthesis of specific proteins in rat liver through changes in the relative abundance of specific mRNAs.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>7854157</pmid><doi>10.1016/0026-0495(95)90299-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Metabolism, clinical and experimental, 1995, Vol.44 (1), p.126-133 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | ADN ANIMALES JOVENES Animals ARN ARN MENSAJERO ARN MESSAGER BINDING PROTEINS Biological and medical sciences BLOOD PROTEINS CARENCE EN OLIGOELEMENT CINC CLONE CLONES COMPLEMENTARY DNA DEFICIENCIA DE OLIGOELEMENTOS DNA Electrophoresis, Gel, Two-Dimensional EXPRESION GENICA EXPRESSION DES GENES FOIE GENE EXPRESSION HIGADO In Vitro Techniques JEUNE ANIMAL LIVER Liver - metabolism Male Medical sciences MESSENGER RNA Metabolic diseases Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) Prealbumin - genetics Protein Biosynthesis PROTEIN SYNTHESIS PROTEINAS AGLUTINANTES PROTEINAS SANGUINEAS PROTEINE DE LIAISON PROTEINE SANGUINE RAT RATA RATS Rats, Inbred Strains Retinol-Binding Proteins - genetics RNA RNA, Messenger - metabolism serum retinol-binding protein SINTESIS DE PROTEINAS SYNTHESE PROTEIQUE TRACE ELEMENT DEFICIENCIES TRANSTHYRETIN weanling rats YOUNG ANIMALS ZINC Zinc - deficiency |
| title | Effects of zinc deficiency on protein synthesis and expression of specific mRNAs in rat liver |
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