Simultaneous activation by 5-azacytidine of intracisternal R particles and murine intracisternal-A particle related sequences in Syrian hamster cells
5-Azacytidine treatment of Syrian hamster (Mesocricetus auratus) cells, BHK21-cl.13, and primary embryo fibroblasts, activates the production of intracisternal type R particles (IRP) as ascertained by electron microscopy scanning and counting. Efficiency of the activation is dose- and time of treatm...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1986-11, Vol.155 (1), p.249-256 |
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| creator | LESSER, J LASNERET, J CANIVET, M EMANOIL-RAVIER, R PERIES, J |
| description | 5-Azacytidine treatment of Syrian hamster (Mesocricetus auratus) cells, BHK21-cl.13, and primary embryo fibroblasts, activates the production of intracisternal type R particles (IRP) as ascertained by electron microscopy scanning and counting. Efficiency of the activation is dose- and time of treatment-dependent. The transcription of mouse intracisternal A particle (IAP) related sequences, which have been described in Syrian hamster genomic DNA, is increased at the same time. This correlates with demethylation of hamster IAP related genes, as shown by restriction of high molecular weight DNA from 5-azacytidine treated cells with methylation sensitive enzymes, and subsequent electrophoresis and molecular hybridization of the fragments with specific DNA probes. In 5-azacytidine treated cells derived from other hamster species (Cricetulus griseus and C. migratorius), intracisternal R particles were not induced, nor were mouse IAP-related RNAs detected. |
| doi_str_mv | 10.1016/0042-6822(86)90184-4 |
| format | Article |
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Efficiency of the activation is dose- and time of treatment-dependent. The transcription of mouse intracisternal A particle (IAP) related sequences, which have been described in Syrian hamster genomic DNA, is increased at the same time. This correlates with demethylation of hamster IAP related genes, as shown by restriction of high molecular weight DNA from 5-azacytidine treated cells with methylation sensitive enzymes, and subsequent electrophoresis and molecular hybridization of the fragments with specific DNA probes. In 5-azacytidine treated cells derived from other hamster species (Cricetulus griseus and C. migratorius), intracisternal R particles were not induced, nor were mouse IAP-related RNAs detected.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(86)90184-4</identifier><identifier>PMID: 2430359</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Action of physical and chemical agents ; Animals ; Azacitidine - pharmacology ; Biological and medical sciences ; Cell Line ; Cricetinae ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Genes, Intracisternal A-Particle ; Inclusion Bodies, Viral - drug effects ; Inclusion Bodies, Viral - ultrastructure ; Methylation ; Microbiology ; Microscopy, Electron ; Proto-Oncogenes ; RNA, Viral - biosynthesis ; Time Factors ; Transcription, Genetic - drug effects ; Virology ; Virus Replication - drug effects</subject><ispartof>Virology (New York, N.Y.), 1986-11, Vol.155 (1), p.249-256</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8039848$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2430359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LESSER, J</creatorcontrib><creatorcontrib>LASNERET, J</creatorcontrib><creatorcontrib>CANIVET, M</creatorcontrib><creatorcontrib>EMANOIL-RAVIER, R</creatorcontrib><creatorcontrib>PERIES, J</creatorcontrib><title>Simultaneous activation by 5-azacytidine of intracisternal R particles and murine intracisternal-A particle related sequences in Syrian hamster cells</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>5-Azacytidine treatment of Syrian hamster (Mesocricetus auratus) cells, BHK21-cl.13, and primary embryo fibroblasts, activates the production of intracisternal type R particles (IRP) as ascertained by electron microscopy scanning and counting. Efficiency of the activation is dose- and time of treatment-dependent. The transcription of mouse intracisternal A particle (IAP) related sequences, which have been described in Syrian hamster genomic DNA, is increased at the same time. This correlates with demethylation of hamster IAP related genes, as shown by restriction of high molecular weight DNA from 5-azacytidine treated cells with methylation sensitive enzymes, and subsequent electrophoresis and molecular hybridization of the fragments with specific DNA probes. In 5-azacytidine treated cells derived from other hamster species (Cricetulus griseus and C. migratorius), intracisternal R particles were not induced, nor were mouse IAP-related RNAs detected.</description><subject>Action of physical and chemical agents</subject><subject>Animals</subject><subject>Azacitidine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes, Intracisternal A-Particle</subject><subject>Inclusion Bodies, Viral - drug effects</subject><subject>Inclusion Bodies, Viral - ultrastructure</subject><subject>Methylation</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>Proto-Oncogenes</subject><subject>RNA, Viral - biosynthesis</subject><subject>Time Factors</subject><subject>Transcription, Genetic - drug effects</subject><subject>Virology</subject><subject>Virus Replication - drug effects</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9KAzEQxoMotVbfQCEHET2sJptsNjmW4j8oCFbPZTabxchuWpOsUN_D9zXFpeDJ0zB8v2-Y-QahU0quKaHihhCeZ0Lm-aUUV4pQyTO-h8aUKJERxuk-Gu-QQ3QUwjtJfVmSERrlnBFWqDH6XtiubyM4s-oDBh3tJ0S7crja4CKDL9CbaGvrDF412LroQdsQjXfQ4me8Bh-tbk1yuhp3vd-Cf6lsuqOwNy1EU-NgPnrjdLJZhxcbb8HhN-i2DqxN24ZjdNBAG8zJUCfo9e72ZfaQzZ_uH2fTebamSsaMa2oqIgvQ6RYBeVMKBgpyqjWnwPLCaAlVJQjooqqLWpQFFJJxVZVMVlSwCbr4nbv2q7RSiMvOhu0Gv3ksy5JyLnLyL0i5UCp9IIFnA9hXnamXa2878JvlEHjSzwcdgoa28eBSVDtMEqYkl-wHtg2TJA</recordid><startdate>19861101</startdate><enddate>19861101</enddate><creator>LESSER, J</creator><creator>LASNERET, J</creator><creator>CANIVET, M</creator><creator>EMANOIL-RAVIER, R</creator><creator>PERIES, J</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19861101</creationdate><title>Simultaneous activation by 5-azacytidine of intracisternal R particles and murine intracisternal-A particle related sequences in Syrian hamster cells</title><author>LESSER, J ; LASNERET, J ; CANIVET, M ; EMANOIL-RAVIER, R ; PERIES, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p198t-4c1eb085ac3596a2f763a9a21cc41a325ec8abb60ac5bd5d675a58349b738b163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Action of physical and chemical agents</topic><topic>Animals</topic><topic>Azacitidine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes, Intracisternal A-Particle</topic><topic>Inclusion Bodies, Viral - drug effects</topic><topic>Inclusion Bodies, Viral - ultrastructure</topic><topic>Methylation</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>Proto-Oncogenes</topic><topic>RNA, Viral - biosynthesis</topic><topic>Time Factors</topic><topic>Transcription, Genetic - drug effects</topic><topic>Virology</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LESSER, J</creatorcontrib><creatorcontrib>LASNERET, J</creatorcontrib><creatorcontrib>CANIVET, M</creatorcontrib><creatorcontrib>EMANOIL-RAVIER, R</creatorcontrib><creatorcontrib>PERIES, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LESSER, J</au><au>LASNERET, J</au><au>CANIVET, M</au><au>EMANOIL-RAVIER, R</au><au>PERIES, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous activation by 5-azacytidine of intracisternal R particles and murine intracisternal-A particle related sequences in Syrian hamster cells</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1986-11-01</date><risdate>1986</risdate><volume>155</volume><issue>1</issue><spage>249</spage><epage>256</epage><pages>249-256</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>5-Azacytidine treatment of Syrian hamster (Mesocricetus auratus) cells, BHK21-cl.13, and primary embryo fibroblasts, activates the production of intracisternal type R particles (IRP) as ascertained by electron microscopy scanning and counting. Efficiency of the activation is dose- and time of treatment-dependent. The transcription of mouse intracisternal A particle (IAP) related sequences, which have been described in Syrian hamster genomic DNA, is increased at the same time. This correlates with demethylation of hamster IAP related genes, as shown by restriction of high molecular weight DNA from 5-azacytidine treated cells with methylation sensitive enzymes, and subsequent electrophoresis and molecular hybridization of the fragments with specific DNA probes. In 5-azacytidine treated cells derived from other hamster species (Cricetulus griseus and C. migratorius), intracisternal R particles were not induced, nor were mouse IAP-related RNAs detected.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>2430359</pmid><doi>10.1016/0042-6822(86)90184-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1986-11, Vol.155 (1), p.249-256 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Action of physical and chemical agents Animals Azacitidine - pharmacology Biological and medical sciences Cell Line Cricetinae Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Genes, Intracisternal A-Particle Inclusion Bodies, Viral - drug effects Inclusion Bodies, Viral - ultrastructure Methylation Microbiology Microscopy, Electron Proto-Oncogenes RNA, Viral - biosynthesis Time Factors Transcription, Genetic - drug effects Virology Virus Replication - drug effects |
| title | Simultaneous activation by 5-azacytidine of intracisternal R particles and murine intracisternal-A particle related sequences in Syrian hamster cells |
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