Eukaryotic Transient-Expression System Based on Recombinant Vaccinia Virus that Synthesizes Bacteriophage T7 RNA Polymerase

DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and stably expressed T7 RNA polymerase in mammalian cells. Target genes were constructed by ins...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-11, Vol.83 (21), p.8122-8126
Hauptverfasser:	Fuerst, Thomas R., Niles, Edward G., Studier, F. William, Moss, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyltransferases - genetics beta-Galactosidase - genetics Biological and medical sciences Biotechnology Chloramphenicol O-Acetyltransferase DNA DNA-Directed RNA Polymerases - biosynthesis DNA-Directed RNA Polymerases - genetics Enzymes Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes Genetic engineering Genetic technics HeLa cells Methods. Procedures. Technologies Plasmids Promoter Regions, Genetic Recombination, Genetic RNA T-Phages - enzymology Transfection Vaccinia Vaccinia virus Vaccinia virus - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons Viruses
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