Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject
A monoclonal human B-lymphoblastoid cell line (UTMB-460) arose spontaneously from the bone marrow of a normal healthy woman who was seropositive for an EB-virus infection. Chromosomally, the UTMB-460 cells are near tetraploid, with a specific translocation (8;9) (p11.2; p24), and have surface IgMk....
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| Veröffentlicht in: | Leukemia research 1986, Vol.10 (10), p.1209-1219 |
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| creator | Juneja, Harinder S. Rajaraman, Sirinivasan Ramsey, Keith M. Elder, Frederick F.B. |
| description | A monoclonal human B-lymphoblastoid cell line (UTMB-460) arose spontaneously from the bone marrow of a normal healthy woman who was seropositive for an EB-virus infection. Chromosomally, the UTMB-460 cells are near tetraploid, with a specific translocation (8;9) (p11.2; p24), and have surface IgMk. The UTMB-460 cells are resistant to killing
in vitro by spontaneous and rIFNα2 and rIL-2 stimulated NK cells from the patient and other normal subjects, but are killed by lymphokine activated killer cells. The index patient has not developed leukemia/lymphoma during the follow-up interval of 22 months. The growth of UTMB-460 cells is supported by undefined growth factors in FCS and by BCGF in the absence of FCS. rIL-2 stimulates DNA synthesis by UTMB-460 cells. The UTMB-460 cells were adherent to the normal MSC in the primary culture and show specific heterotypic adherence to normal MSC when compared to skin fibroblasts. In addition, 6/6 normal marrow stromal cells and 4/6 normal skin fibroblasts induced growth of colonies from UTMB-460 cells. These data suggest that MSC interacted with the transformed cells (UTMB-460)
in vitro and played a critical role in the establishment of the UTMB-460 cell line. |
| doi_str_mv | 10.1016/0145-2126(86)90239-0 |
| format | Article |
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in vitro by spontaneous and rIFNα2 and rIL-2 stimulated NK cells from the patient and other normal subjects, but are killed by lymphokine activated killer cells. The index patient has not developed leukemia/lymphoma during the follow-up interval of 22 months. The growth of UTMB-460 cells is supported by undefined growth factors in FCS and by BCGF in the absence of FCS. rIL-2 stimulates DNA synthesis by UTMB-460 cells. The UTMB-460 cells were adherent to the normal MSC in the primary culture and show specific heterotypic adherence to normal MSC when compared to skin fibroblasts. In addition, 6/6 normal marrow stromal cells and 4/6 normal skin fibroblasts induced growth of colonies from UTMB-460 cells. These data suggest that MSC interacted with the transformed cells (UTMB-460)
in vitro and played a critical role in the establishment of the UTMB-460 cell line.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/0145-2126(86)90239-0</identifier><identifier>PMID: 3022081</identifier><identifier>CODEN: LEREDD</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; B lymphocytes ; B-Lymphocytes - immunology ; B-Lymphocytes - pathology ; B-Lymphocytes - ultrastructure ; Biological and medical sciences ; Bone Marrow - physiology ; Cell Adhesion ; Cell Communication ; Cell Line ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Cell Transformation, Viral ; DNA - biosynthesis ; Epstein-Barr virus ; Female ; Fundamental and applied biological sciences. Psychology ; Herpesvirus 4, Human ; human ; Humans ; immune-surveillance against malignant cells ; Leukemia - pathology ; lymphoblastic leukemia ; malignancy ; Marrow stromal cells ; Molecular and cellular biology ; transformation</subject><ispartof>Leukemia research, 1986, Vol.10 (10), p.1209-1219</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-c9c66ddce0b76df24769ed193e4d6ca0d7320b32dafddc61229806fa650b18b53</citedby><cites>FETCH-LOGICAL-c386t-c9c66ddce0b76df24769ed193e4d6ca0d7320b32dafddc61229806fa650b18b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0145-2126(86)90239-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27910,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7913403$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3022081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juneja, Harinder S.</creatorcontrib><creatorcontrib>Rajaraman, Sirinivasan</creatorcontrib><creatorcontrib>Ramsey, Keith M.</creatorcontrib><creatorcontrib>Elder, Frederick F.B.</creatorcontrib><title>Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>A monoclonal human B-lymphoblastoid cell line (UTMB-460) arose spontaneously from the bone marrow of a normal healthy woman who was seropositive for an EB-virus infection. Chromosomally, the UTMB-460 cells are near tetraploid, with a specific translocation (8;9) (p11.2; p24), and have surface IgMk. The UTMB-460 cells are resistant to killing
in vitro by spontaneous and rIFNα2 and rIL-2 stimulated NK cells from the patient and other normal subjects, but are killed by lymphokine activated killer cells. The index patient has not developed leukemia/lymphoma during the follow-up interval of 22 months. The growth of UTMB-460 cells is supported by undefined growth factors in FCS and by BCGF in the absence of FCS. rIL-2 stimulates DNA synthesis by UTMB-460 cells. The UTMB-460 cells were adherent to the normal MSC in the primary culture and show specific heterotypic adherence to normal MSC when compared to skin fibroblasts. In addition, 6/6 normal marrow stromal cells and 4/6 normal skin fibroblasts induced growth of colonies from UTMB-460 cells. These data suggest that MSC interacted with the transformed cells (UTMB-460)
in vitro and played a critical role in the establishment of the UTMB-460 cell line.</description><subject>Adult</subject><subject>B lymphocytes</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - pathology</subject><subject>B-Lymphocytes - ultrastructure</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - physiology</subject><subject>Cell Adhesion</subject><subject>Cell Communication</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Cell Transformation, Viral</subject><subject>DNA - biosynthesis</subject><subject>Epstein-Barr virus</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesvirus 4, Human</subject><subject>human</subject><subject>Humans</subject><subject>immune-surveillance against malignant cells</subject><subject>Leukemia - pathology</subject><subject>lymphoblastic leukemia</subject><subject>malignancy</subject><subject>Marrow stromal cells</subject><subject>Molecular and cellular biology</subject><subject>transformation</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEURoMoY8_oP1DIQkQXpXlU5bERdBgfMCCIrkMquaEzpCptUqXOvzdlN710dRf3fB_3HoSeUfKGEireEtoPHaNMvFLitSaM6448QDuqJO8GxYeHaHdGHqPLWu8IIYOm-gJdcMIYUXSH_nzLCXAOeLKl5N-4LiVPNmEHKVUcZ7zsAUNd7Jhi3U8wLxts8VLsXEMuE3ic7qfDPo_J1iU6_KHbsjjFGXBoZQ2eG9c69-tkZ1zX8Q7c8gQ9CjZVeHqaV-jHx5vv15-726-fvly_v-0cV2LpnHZCeO-AjFL4wHopNHiqOfReOEu85IyMnHkbGiUoY1oREawYyEjVOPAr9PLYeyj559o-MVOs24V2hrxWIyXlUsm-gf0RdCXXWiCYQ4nNyr2hxGzCzWbTbDaNEuafcENa7Pmpfx2bjHPoZLjtX5z2tjqbQvPmYj1jUlPeE96wd0cMmotfEYqpLsLswMfSbBmf4__v-Av-2528</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Juneja, Harinder S.</creator><creator>Rajaraman, Sirinivasan</creator><creator>Ramsey, Keith M.</creator><creator>Elder, Frederick F.B.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject</title><author>Juneja, Harinder S. ; Rajaraman, Sirinivasan ; Ramsey, Keith M. ; Elder, Frederick F.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-c9c66ddce0b76df24769ed193e4d6ca0d7320b32dafddc61229806fa650b18b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adult</topic><topic>B lymphocytes</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - pathology</topic><topic>B-Lymphocytes - ultrastructure</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - physiology</topic><topic>Cell Adhesion</topic><topic>Cell Communication</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Cell Transformation, Viral</topic><topic>DNA - biosynthesis</topic><topic>Epstein-Barr virus</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpesvirus 4, Human</topic><topic>human</topic><topic>Humans</topic><topic>immune-surveillance against malignant cells</topic><topic>Leukemia - pathology</topic><topic>lymphoblastic leukemia</topic><topic>malignancy</topic><topic>Marrow stromal cells</topic><topic>Molecular and cellular biology</topic><topic>transformation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juneja, Harinder S.</creatorcontrib><creatorcontrib>Rajaraman, Sirinivasan</creatorcontrib><creatorcontrib>Ramsey, Keith M.</creatorcontrib><creatorcontrib>Elder, Frederick F.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juneja, Harinder S.</au><au>Rajaraman, Sirinivasan</au><au>Ramsey, Keith M.</au><au>Elder, Frederick F.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>1986</date><risdate>1986</risdate><volume>10</volume><issue>10</issue><spage>1209</spage><epage>1219</epage><pages>1209-1219</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><coden>LEREDD</coden><abstract>A monoclonal human B-lymphoblastoid cell line (UTMB-460) arose spontaneously from the bone marrow of a normal healthy woman who was seropositive for an EB-virus infection. Chromosomally, the UTMB-460 cells are near tetraploid, with a specific translocation (8;9) (p11.2; p24), and have surface IgMk. The UTMB-460 cells are resistant to killing
in vitro by spontaneous and rIFNα2 and rIL-2 stimulated NK cells from the patient and other normal subjects, but are killed by lymphokine activated killer cells. The index patient has not developed leukemia/lymphoma during the follow-up interval of 22 months. The growth of UTMB-460 cells is supported by undefined growth factors in FCS and by BCGF in the absence of FCS. rIL-2 stimulates DNA synthesis by UTMB-460 cells. The UTMB-460 cells were adherent to the normal MSC in the primary culture and show specific heterotypic adherence to normal MSC when compared to skin fibroblasts. In addition, 6/6 normal marrow stromal cells and 4/6 normal skin fibroblasts induced growth of colonies from UTMB-460 cells. These data suggest that MSC interacted with the transformed cells (UTMB-460)
in vitro and played a critical role in the establishment of the UTMB-460 cell line.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>3022081</pmid><doi>10.1016/0145-2126(86)90239-0</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0145-2126 |
| ispartof | Leukemia research, 1986, Vol.10 (10), p.1209-1219 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Adult B lymphocytes B-Lymphocytes - immunology B-Lymphocytes - pathology B-Lymphocytes - ultrastructure Biological and medical sciences Bone Marrow - physiology Cell Adhesion Cell Communication Cell Line Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cell Transformation, Viral DNA - biosynthesis Epstein-Barr virus Female Fundamental and applied biological sciences. Psychology Herpesvirus 4, Human human Humans immune-surveillance against malignant cells Leukemia - pathology lymphoblastic leukemia malignancy Marrow stromal cells Molecular and cellular biology transformation |
| title | Role of marrow stromal cells in the establishment of a transformed lymphoblastic B-cell line from a normal human subject |
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