Glycosylated Peptide Hormones: Pharmacological Properties and Conformational Studies of Analogs of [1-Desamino,8-D-arginine]vasopressin

Two analogues of the antidiuretic drug [1-desamino,8-D-arginine]vasopressin (DDAVP), which have a glycosylated serine at position 4, have been prepared by Fmoc solid phase peptide synthesis. The glycosylated analogues had significantly higher bioavailabilities than the nonglycosylated [D-Tyr2,Ser4]D...

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Veröffentlicht in:Journal of medicinal chemistry 1995-01, Vol.38 (1), p.161-169
Hauptverfasser: Kihlberg, Jan, Aahman, Jens, Walse, Bjoern, Drakenberg, Torbjoern, Nilsson, Anders, Soederberg-Ahlm, Christina, Bengtsson, Bengt, Olsson, Haakan
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container_issue 1
container_start_page 161
container_title Journal of medicinal chemistry
container_volume 38
creator Kihlberg, Jan
Aahman, Jens
Walse, Bjoern
Drakenberg, Torbjoern
Nilsson, Anders
Soederberg-Ahlm, Christina
Bengtsson, Bengt
Olsson, Haakan
description Two analogues of the antidiuretic drug [1-desamino,8-D-arginine]vasopressin (DDAVP), which have a glycosylated serine at position 4, have been prepared by Fmoc solid phase peptide synthesis. The glycosylated analogues had significantly higher bioavailabilities than the nonglycosylated [D-Tyr2,Ser4]DDAVP and DDAVP on intraintestinal administration in rat. The improved bioavailability resulted from an increased absorption from the small intestine and most likely from an increased stability toward enzymatic degradation, whereas plasma clearance was either unaffected or slightly increased by the glycosylation. The glycosylated analogues displayed only very low agonistic and antagonistic activities at the vasopressin V2-receptor. Conformational studies performed by 1H NMR spectroscopy did not reveal any major influence from glycosylation on the conformation of the peptide backbone. The lack of receptor binding displayed by the analogues is therefore most likely explained by steric repulsion between the carbohydrate moiety and the vasopressin receptor which prevents receptor binding.
doi_str_mv 10.1021/jm00001a021
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The glycosylated analogues had significantly higher bioavailabilities than the nonglycosylated [D-Tyr2,Ser4]DDAVP and DDAVP on intraintestinal administration in rat. The improved bioavailability resulted from an increased absorption from the small intestine and most likely from an increased stability toward enzymatic degradation, whereas plasma clearance was either unaffected or slightly increased by the glycosylation. The glycosylated analogues displayed only very low agonistic and antagonistic activities at the vasopressin V2-receptor. Conformational studies performed by 1H NMR spectroscopy did not reveal any major influence from glycosylation on the conformation of the peptide backbone. 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Med. Chem</addtitle><description>Two analogues of the antidiuretic drug [1-desamino,8-D-arginine]vasopressin (DDAVP), which have a glycosylated serine at position 4, have been prepared by Fmoc solid phase peptide synthesis. The glycosylated analogues had significantly higher bioavailabilities than the nonglycosylated [D-Tyr2,Ser4]DDAVP and DDAVP on intraintestinal administration in rat. The improved bioavailability resulted from an increased absorption from the small intestine and most likely from an increased stability toward enzymatic degradation, whereas plasma clearance was either unaffected or slightly increased by the glycosylation. The glycosylated analogues displayed only very low agonistic and antagonistic activities at the vasopressin V2-receptor. Conformational studies performed by 1H NMR spectroscopy did not reveal any major influence from glycosylation on the conformation of the peptide backbone. 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subjects Amino Acid Sequence
Animals
Biological and medical sciences
Biological Availability
Chymotrypsin - pharmacology
Deamino Arginine Vasopressin - analogs & derivatives
Drug Stability
Glycoproteins - chemistry
Glycoproteins - metabolism
Glycoproteins - pharmacology
Glycosylation
Hormones. Endocrine system
Intestinal Absorption
Intestine, Small - metabolism
Male
Medical sciences
Models, Molecular
Molecular Sequence Data
Pharmacology. Drug treatments
Protein Conformation
Rats
Rats, Sprague-Dawley
Structure-Activity Relationship
Vasopressins - chemistry
Vasopressins - metabolism
Vasopressins - pharmacology
title Glycosylated Peptide Hormones: Pharmacological Properties and Conformational Studies of Analogs of [1-Desamino,8-D-arginine]vasopressin
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