Metabolic radiolabelling of Mycoplasma gallisepticum on Vero cells and radioimmunoprecipitation assay

A monoclonal antibody (MAb) A2 was produced against a major polypeptide of Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10 4.33 and had a titre of 10 4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using...

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Veröffentlicht in:	Journal of immunological methods 1995, Vol.178 (1), p.53-58
Hauptverfasser:	Silim, A., Kheyar, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigenic determinants, haptens, artificial antigens Antigens Antigens, Bacterial - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cercopithecus aethiops Fundamental and applied biological sciences. Psychology Fundamental immunology Isotope Labeling Methionine Microbiology Molecular immunology Monoclonal antibody Mycoplasma - immunology Mycoplasma gallisepticum Radioimmunoprecipitation Radioimmunoprecipitation Assay - methods Sulfur Radioisotopes Vero cell Vero Cells
Online-Zugang:	Volltext
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