Metabolic radiolabelling of Mycoplasma gallisepticum on Vero cells and radioimmunoprecipitation assay
A monoclonal antibody (MAb) A2 was produced against a major polypeptide of Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10 4.33 and had a titre of 10 4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using...
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| Veröffentlicht in: | Journal of immunological methods 1995, Vol.178 (1), p.53-58 |
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| container_title | Journal of immunological methods |
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| creator | Silim, A. Kheyar, A. |
| description | A monoclonal antibody (MAb) A2 was produced against a major polypeptide of
Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10
4.33 and had a titre of 10
4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using metabolic [
35S]methionine radiolabelling of
M. gallisepticum suspension in Vero cell culture, MAb A2 was able to precipitate the 64 kDa protein and another protein of 47 kDa. The present study involving [
35S]methionine labelling of
M. gallisepticum in Vero cells represents a novel approach for labelling and characterizing the conformation-dependent mycoplasmal antigens in a RIPA system. |
| doi_str_mv | 10.1016/0022-1759(94)00240-W |
| format | Article |
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Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10
4.33 and had a titre of 10
4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using metabolic [
35S]methionine radiolabelling of
M. gallisepticum suspension in Vero cell culture, MAb A2 was able to precipitate the 64 kDa protein and another protein of 47 kDa. The present study involving [
35S]methionine labelling of
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Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10
4.33 and had a titre of 10
4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using metabolic [
35S]methionine radiolabelling of
M. gallisepticum suspension in Vero cell culture, MAb A2 was able to precipitate the 64 kDa protein and another protein of 47 kDa. The present study involving [
35S]methionine labelling of
M. gallisepticum in Vero cells represents a novel approach for labelling and characterizing the conformation-dependent mycoplasmal antigens in a RIPA system.</description><subject>Animals</subject><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cercopithecus aethiops</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Isotope Labeling</subject><subject>Methionine</subject><subject>Microbiology</subject><subject>Molecular immunology</subject><subject>Monoclonal antibody</subject><subject>Mycoplasma - immunology</subject><subject>Mycoplasma gallisepticum</subject><subject>Radioimmunoprecipitation</subject><subject>Radioimmunoprecipitation Assay - methods</subject><subject>Sulfur Radioisotopes</subject><subject>Vero cell</subject><subject>Vero Cells</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVJSTZpv0ECPoSQHNxoZNmWLoGw9B9s6CVtjmIsyUHBthzJLuy3r9xd9pichpn5veHxhpBzoF-AQnVLKWM51KW8lvwmNZzmTx_ICkTN8lrS8oisDsgJOY3xhVIKtKLH5LgWTIqqXBH7YCdsfOd0FtA432Fju84Nz5lvs4et9mOHscfsGdM02nFyeu4zP2R_bPCZTmzMcDA7sev7efBjsNqNbsLJJQ5jxO0n8rHFLtrP-3pGfn_7-rj-kW9-ff-5vt_kuijYlLdgGg0WDeWiQd4wKgFYVaJEVkLZlobJyoiqkbURTHNok1tRVJw1WhsGxRm52t0dg3-dbZxU7-JiEgfr56jqGgoAQd8FoaoFL2WRQL4DdfAxBtuqMbgew1YBVcsb1JKxWjJWkqv_b1BPSXaxvz83vTUH0T73tL_c7zFq7NqAg3bxgBWFZIyyhN3tMJtC--tsUFE7O2hrXAp5Usa7t338A6iNpXM</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Silim, A.</creator><creator>Kheyar, A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Metabolic radiolabelling of Mycoplasma gallisepticum on Vero cells and radioimmunoprecipitation assay</title><author>Silim, A. ; Kheyar, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-f1dbc1ead048ba4b20911265a9a2515f5d296d86b97d82c41fabe83642bccd213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cercopithecus aethiops</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Isotope Labeling</topic><topic>Methionine</topic><topic>Microbiology</topic><topic>Molecular immunology</topic><topic>Monoclonal antibody</topic><topic>Mycoplasma - immunology</topic><topic>Mycoplasma gallisepticum</topic><topic>Radioimmunoprecipitation</topic><topic>Radioimmunoprecipitation Assay - methods</topic><topic>Sulfur Radioisotopes</topic><topic>Vero cell</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silim, A.</creatorcontrib><creatorcontrib>Kheyar, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silim, A.</au><au>Kheyar, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic radiolabelling of Mycoplasma gallisepticum on Vero cells and radioimmunoprecipitation assay</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1995</date><risdate>1995</risdate><volume>178</volume><issue>1</issue><spage>53</spage><epage>58</epage><pages>53-58</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A monoclonal antibody (MAb) A2 was produced against a major polypeptide of
Mycoplasma gallisepticum with a molecular mass of 64 kDa. MAb A2 reacted in immunoblot at a titre of 10
4.33 and had a titre of 10
4.5 in an enzyme-linked immunosorbent assay. In a radioimmunoprecipitation assay (RIPA) using metabolic [
35S]methionine radiolabelling of
M. gallisepticum suspension in Vero cell culture, MAb A2 was able to precipitate the 64 kDa protein and another protein of 47 kDa. The present study involving [
35S]methionine labelling of
M. gallisepticum in Vero cells represents a novel approach for labelling and characterizing the conformation-dependent mycoplasmal antigens in a RIPA system.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>7829865</pmid><doi>10.1016/0022-1759(94)00240-W</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1995, Vol.178 (1), p.53-58 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_77131180 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antigenic determinants, haptens, artificial antigens Antigens Antigens, Bacterial - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cercopithecus aethiops Fundamental and applied biological sciences. Psychology Fundamental immunology Isotope Labeling Methionine Microbiology Molecular immunology Monoclonal antibody Mycoplasma - immunology Mycoplasma gallisepticum Radioimmunoprecipitation Radioimmunoprecipitation Assay - methods Sulfur Radioisotopes Vero cell Vero Cells |
| title | Metabolic radiolabelling of Mycoplasma gallisepticum on Vero cells and radioimmunoprecipitation assay |
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