Protein Expression, Selective Isotopic Labeling, and Analysis of Hyperfine-Shifted NMR Signals of Anabaena 7120 Vegetative [2Fe-2S]Ferredoxin

Two alternative T7 RNA promoter/polymerase systems have been employed for the heterologous expression of a plant-type [2Fe-2S]ferredoxin, Anabaena 7120 vegetative ferredoxin, in Escherichia coli at high levels (∼20 mg/liter of culture). One system was used when 15N-labeling the ferredoxin uniformly...

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