Fibronectin synthesis and degradation in human fibroblasts with aging
Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. Th...
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| Veröffentlicht in: | Mechanisms of ageing and development 1986-08, Vol.35 (3), p.221-232 |
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| container_title | Mechanisms of ageing and development |
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| creator | Shevitz, Jerry Jenkins, C.S.P. Hatcher, Victor B. |
| description | Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. The absolute protein concentration increased in the late passaged fibroblasts. There was no significant difference in the [
3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase rate of protein synthesis/cell. |
| doi_str_mv | 10.1016/0047-6374(86)90125-9 |
| format | Article |
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3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase rate of protein synthesis/cell.</description><identifier>ISSN: 0047-6374</identifier><identifier>EISSN: 1872-6216</identifier><identifier>DOI: 10.1016/0047-6374(86)90125-9</identifier><identifier>PMID: 3773572</identifier><identifier>CODEN: MAGDA3</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Ageing, cell death ; Aging ; Biological and medical sciences ; Cell physiology ; Cell Survival ; Cells, Cultured ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Fibronectin synthesis ; Fibronectins - biosynthesis ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Molecular and cellular biology ; Penis ; Protein Biosynthesis ; Protein synthesis</subject><ispartof>Mechanisms of ageing and development, 1986-08, Vol.35 (3), p.221-232</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-959381fd508016a535daf176f0de523170ab2e3ee11efe79fec5d357a776f3b63</citedby><cites>FETCH-LOGICAL-c386t-959381fd508016a535daf176f0de523170ab2e3ee11efe79fec5d357a776f3b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0047637486901259$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8083831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3773572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shevitz, Jerry</creatorcontrib><creatorcontrib>Jenkins, C.S.P.</creatorcontrib><creatorcontrib>Hatcher, Victor B.</creatorcontrib><title>Fibronectin synthesis and degradation in human fibroblasts with aging</title><title>Mechanisms of ageing and development</title><addtitle>Mech Ageing Dev</addtitle><description>Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. The absolute protein concentration increased in the late passaged fibroblasts. There was no significant difference in the [
3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase rate of protein synthesis/cell.</description><subject>Ageing, cell death</subject><subject>Aging</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Fibronectin synthesis</subject><subject>Fibronectins - biosynthesis</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Penis</subject><subject>Protein Biosynthesis</subject><subject>Protein synthesis</subject><issn>0047-6374</issn><issn>1872-6216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotX78A4U9iOhhNdk0H3sRRKwKghc9h2wyaSPbbE22Sv-9WVt69DSH93mHmQehM4JvCCb8FuOJKDkVkyvJr2tMKlbWe2hMpKhKXhG-j8Y75BAdpfSJMSaTio_QiApBmajG6HHqm9gFML0PRVqHfg7Jp0IHW1iYRW1177tQ5HC-WuhQuAFvWp36VPz4fl7omQ-zE3TgdJvgdDuP0cf08f3huXx9e3p5uH8tDZW8L2tWU0mcZVjmBzSjzGpHBHfYAqsoEVg3FVAAQsCBqB0YZvOdWmSGNpweo8vN3mXsvlaQerXwyUDb6gDdKikhSCUIG8DJBjSxSymCU8voFzquFcFqsKcGNWpQoyRXf_ZUnWvn2_2rZgF2V9rqyvnFNtfJ6NZFHYxPO0xiSSUlGbvbYJBdfHuIKhkPwYD1MZtWtvP_3_ELoBqLEg</recordid><startdate>19860801</startdate><enddate>19860801</enddate><creator>Shevitz, Jerry</creator><creator>Jenkins, C.S.P.</creator><creator>Hatcher, Victor B.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860801</creationdate><title>Fibronectin synthesis and degradation in human fibroblasts with aging</title><author>Shevitz, Jerry ; Jenkins, C.S.P. ; Hatcher, Victor B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-959381fd508016a535daf176f0de523170ab2e3ee11efe79fec5d357a776f3b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Ageing, cell death</topic><topic>Aging</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Fibronectin synthesis</topic><topic>Fibronectins - biosynthesis</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Penis</topic><topic>Protein Biosynthesis</topic><topic>Protein synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shevitz, Jerry</creatorcontrib><creatorcontrib>Jenkins, C.S.P.</creatorcontrib><creatorcontrib>Hatcher, Victor B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of ageing and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shevitz, Jerry</au><au>Jenkins, C.S.P.</au><au>Hatcher, Victor B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibronectin synthesis and degradation in human fibroblasts with aging</atitle><jtitle>Mechanisms of ageing and development</jtitle><addtitle>Mech Ageing Dev</addtitle><date>1986-08-01</date><risdate>1986</risdate><volume>35</volume><issue>3</issue><spage>221</spage><epage>232</epage><pages>221-232</pages><issn>0047-6374</issn><eissn>1872-6216</eissn><coden>MAGDA3</coden><abstract>Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. The absolute protein concentration increased in the late passaged fibroblasts. There was no significant difference in the [
3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase rate of protein synthesis/cell.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>3773572</pmid><doi>10.1016/0047-6374(86)90125-9</doi><tpages>12</tpages></addata></record> |
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| ispartof | Mechanisms of ageing and development, 1986-08, Vol.35 (3), p.221-232 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Ageing, cell death Aging Biological and medical sciences Cell physiology Cell Survival Cells, Cultured Fibroblasts - cytology Fibroblasts - metabolism Fibronectin synthesis Fibronectins - biosynthesis Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Humans Male Molecular and cellular biology Penis Protein Biosynthesis Protein synthesis |
| title | Fibronectin synthesis and degradation in human fibroblasts with aging |
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