Analysis of an initial step of T cell adhesion to endothelial monolayers under flow conditions
We analyzed an initial step of T cell adhesion to endothelial cells under flow conditions. An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelia...
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| Veröffentlicht in: | The Journal of immunology (1950) 1995-02, Vol.154 (3), p.1216-1222 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 154 |
| creator | Yago, T Tsukuda, M Yamazaki, H Nishi, T Amano, T Minami, M |
| description | We analyzed an initial step of T cell adhesion to endothelial cells under flow conditions. An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelial cells under flow conditions. The rolling velocity of T cells on endothelial cells increased after treatment of endothelial cells with anti-E- or P-selectin Abs. Also, rolling of T cells was observed on E-selectin-transfected CHO cells but not on ICAM-1-transfected CHO cells. Thus, rolling of T cells under flow condition was dependent on E-selectin as well as P-selectin expressed on endothelial cells. In contrast, neither anti-E- nor P-selectin Abs inhibited the immediate arrest of T cells on endothelial cells significantly and anti-ICAM-1 Abs also failed to inhibit the immediate arrest of T cells. Therefore, other adhesion molecules may play an important role in the immediate arrest of T cells under flow conditions. The number of CD45RA- T cells rolling on E-selectin-transfected CHO cells was much higher than that of CD45RA+ T cells. |
| doi_str_mv | 10.4049/jimmunol.154.3.1216 |
| format | Article |
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An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelial cells under flow conditions. The rolling velocity of T cells on endothelial cells increased after treatment of endothelial cells with anti-E- or P-selectin Abs. Also, rolling of T cells was observed on E-selectin-transfected CHO cells but not on ICAM-1-transfected CHO cells. Thus, rolling of T cells under flow condition was dependent on E-selectin as well as P-selectin expressed on endothelial cells. In contrast, neither anti-E- nor P-selectin Abs inhibited the immediate arrest of T cells on endothelial cells significantly and anti-ICAM-1 Abs also failed to inhibit the immediate arrest of T cells. Therefore, other adhesion molecules may play an important role in the immediate arrest of T cells under flow conditions. 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An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelial cells under flow conditions. The rolling velocity of T cells on endothelial cells increased after treatment of endothelial cells with anti-E- or P-selectin Abs. Also, rolling of T cells was observed on E-selectin-transfected CHO cells but not on ICAM-1-transfected CHO cells. Thus, rolling of T cells under flow condition was dependent on E-selectin as well as P-selectin expressed on endothelial cells. In contrast, neither anti-E- nor P-selectin Abs inhibited the immediate arrest of T cells on endothelial cells significantly and anti-ICAM-1 Abs also failed to inhibit the immediate arrest of T cells. Therefore, other adhesion molecules may play an important role in the immediate arrest of T cells under flow conditions. The number of CD45RA- T cells rolling on E-selectin-transfected CHO cells was much higher than that of CD45RA+ T cells.</description><subject>Animals</subject><subject>Cell Adhesion - immunology</subject><subject>Cell Adhesion Molecules - immunology</subject><subject>Cell Movement - immunology</subject><subject>Cells, Cultured</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>E-Selectin</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - immunology</subject><subject>Humans</subject><subject>Intercellular Adhesion Molecule-1 - immunology</subject><subject>Leukocyte Common Antigens - immunology</subject><subject>P-Selectin</subject><subject>Platelet Membrane Glycoproteins - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Transfection</subject><subject>Umbilical Cord - cytology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotVZ_gQg56WlrPjbJ5ljELyh4qVdDms3alGxSN7ss_ffu0irePA3MPPPO8ABwjdE8R7m837q67kL0c8zyOZ1jgvkJmGLGUMY54qdgihAhGRZcnIOLlLYIIY5IPgETwYgUkk3BxyJov08uwVhBHaALrnXaw9Ta3dhaQWO9h7rc2ORigG2ENpSx3Vg_YnUc7uu9bRLsQmkbWPnYQxNDOcTEkC7BWaV9slfHOgPvT4-rh5ds-fb8-rBYZiZHRZtxI4hmYl1VptBG0CIvMOX5WvJcMItLLDC1lJACUWIQlVoTVFhDTCG5lJLRGbg95O6a-NXZ1KrapfFzHWzskhICEyol_xfEnEuEGR5AegBNE1NqbKV2jat1s1cYqVG_-tGvBv2KqlH_sHVzjO_WtS1_d46-h_ndYb5xn5veNValWns_0Fj1ff8n6RsRZJBJ</recordid><startdate>19950201</startdate><enddate>19950201</enddate><creator>Yago, T</creator><creator>Tsukuda, M</creator><creator>Yamazaki, H</creator><creator>Nishi, T</creator><creator>Amano, T</creator><creator>Minami, M</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950201</creationdate><title>Analysis of an initial step of T cell adhesion to endothelial monolayers under flow conditions</title><author>Yago, T ; Tsukuda, M ; Yamazaki, H ; Nishi, T ; Amano, T ; Minami, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-6c72a57bffc8ac738481364b96475e1d1713e3228032c039aa208ec2c89699953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Cell Adhesion - immunology</topic><topic>Cell Adhesion Molecules - immunology</topic><topic>Cell Movement - immunology</topic><topic>Cells, Cultured</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>E-Selectin</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - immunology</topic><topic>Humans</topic><topic>Intercellular Adhesion Molecule-1 - immunology</topic><topic>Leukocyte Common Antigens - immunology</topic><topic>P-Selectin</topic><topic>Platelet Membrane Glycoproteins - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Transfection</topic><topic>Umbilical Cord - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yago, T</creatorcontrib><creatorcontrib>Tsukuda, M</creatorcontrib><creatorcontrib>Yamazaki, H</creatorcontrib><creatorcontrib>Nishi, T</creatorcontrib><creatorcontrib>Amano, T</creatorcontrib><creatorcontrib>Minami, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yago, T</au><au>Tsukuda, M</au><au>Yamazaki, H</au><au>Nishi, T</au><au>Amano, T</au><au>Minami, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of an initial step of T cell adhesion to endothelial monolayers under flow conditions</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1995-02-01</date><risdate>1995</risdate><volume>154</volume><issue>3</issue><spage>1216</spage><epage>1222</epage><pages>1216-1222</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>We analyzed an initial step of T cell adhesion to endothelial cells under flow conditions. An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelial cells under flow conditions. The rolling velocity of T cells on endothelial cells increased after treatment of endothelial cells with anti-E- or P-selectin Abs. Also, rolling of T cells was observed on E-selectin-transfected CHO cells but not on ICAM-1-transfected CHO cells. Thus, rolling of T cells under flow condition was dependent on E-selectin as well as P-selectin expressed on endothelial cells. In contrast, neither anti-E- nor P-selectin Abs inhibited the immediate arrest of T cells on endothelial cells significantly and anti-ICAM-1 Abs also failed to inhibit the immediate arrest of T cells. Therefore, other adhesion molecules may play an important role in the immediate arrest of T cells under flow conditions. 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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1995-02, Vol.154 (3), p.1216-1222 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Adhesion - immunology Cell Adhesion Molecules - immunology Cell Movement - immunology Cells, Cultured CHO Cells Cricetinae E-Selectin Endothelium, Vascular - cytology Endothelium, Vascular - immunology Humans Intercellular Adhesion Molecule-1 - immunology Leukocyte Common Antigens - immunology P-Selectin Platelet Membrane Glycoproteins - immunology T-Lymphocytes - immunology Transfection Umbilical Cord - cytology |
| title | Analysis of an initial step of T cell adhesion to endothelial monolayers under flow conditions |
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