A simple indirect immunofluorescence micromethod for cell typing

We describe a simple indirect immunofluorescence micromethod for cell typing. Cells are layered on 18-well immunofluorescence slides coated with high polymer poly- L-lysine. Expressed cell surface membrane markers are detected by indirect immunofluorescence using monoclonal antibodies and the cells...

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Veröffentlicht in:	Journal of immunological methods 1986-11, Vol.93 (2), p.217-223
Hauptverfasser:	Joly, P., Devillier, P., Pradier, O., Le Vagueresse, R., Reguer, M., Rougier, Y., Saliou, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Biological and medical sciences Bronchoalveolar lavage fluid Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Immunological reactions in vitro Lung - immunology Lymphocyte typing Lymphocytes - classification Lymphocytes - immunology Methods Mice Micromethod Monoclonal antibody Poly- L-lysine-coated slides
Online-Zugang:	Volltext
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