[3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions
: [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We...
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| Veröffentlicht in: | Journal of neurochemistry 1995-02, Vol.64 (2), p.940-943 |
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| container_title | Journal of neurochemistry |
| container_volume | 64 |
| creator | Vile, John M. D'Souza, Ursula M. Strange, Philip G. |
| description | : [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of |
| doi_str_mv | 10.1046/j.1471-4159.1995.64020940.x |
| format | Article |
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We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of <20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [3H]spiperone and [3H]nemonapride do not differentiate between different forms or populations of D2‐like receptors.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.1995.64020940.x</identifier><identifier>PMID: 7830089</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Antagonist binding ; Benzamides - metabolism ; Binding Sites ; Binding, Competitive ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; CHO Cells ; Cricetinae ; D2 dopamine receptor ; D3 dopamine receptor ; Dopamine Antagonists - metabolism ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) ; Rats ; Receptors, Dopamine - metabolism ; Receptors, Dopamine D2 - metabolism ; Receptors, Dopamine D3 ; Recombination, Genetic ; Spiperone - metabolism ; Tritium</subject><ispartof>Journal of neurochemistry, 1995-02, Vol.64 (2), p.940-943</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.1995.64020940.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.1995.64020940.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3437250$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7830089$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vile, John M.</creatorcontrib><creatorcontrib>D'Souza, Ursula M.</creatorcontrib><creatorcontrib>Strange, Philip G.</creatorcontrib><title>[3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of <20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [3H]spiperone and [3H]nemonapride do not differentiate between different forms or populations of D2‐like receptors.</description><subject>Animals</subject><subject>Antagonist binding</subject><subject>Benzamides - metabolism</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>D2 dopamine receptor</subject><subject>D3 dopamine receptor</subject><subject>Dopamine Antagonists - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</subject><subject>Rats</subject><subject>Receptors, Dopamine - metabolism</subject><subject>Receptors, Dopamine D2 - metabolism</subject><subject>Receptors, Dopamine D3</subject><subject>Recombination, Genetic</subject><subject>Spiperone - metabolism</subject><subject>Tritium</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kdtu1DAURS0EKkPhE5AsgXhL8DWOH9GkpaDRVOrlCSHLiU-QR4mdxhPafgW_TNJO58my1_KRvTdCnyjJKRHF111OhaKZoFLnVGuZF4IwogXJH16h1ZG9RitCGMs4EewtepfSjhBaiIKeoBNVckJKvUL_fvGL31voY7DD6B1gGxxezq4HP8AYA-CNraHDZ3eT_2s7CHu8nfoaxoRjiyv2dKHiuIqD7f2sX0EDwz7O3Ads8ZUNf2BRb3xKE6Qn_zY4GHHl2xbGZeI6Buf3Pob0Hr1pbZfgw2E9RbfnZzfri2xz-f3H-tsmGxilJFMltbVmtZWUSal5XZQlVWz-ekugqBtXEFFT3TaNkNw53XKuoNSutUI2par5KfryPHcY4938rL3pfWqg62yAOCWjFJ0jlXwWPx7Eqe7BmTml3o6P5pDgzD8fuE2N7drRhsano8YFV0ySWauetXvfweMRU2KWSs3OLLWZpTazVGpeKjUP5ud2_bLj_wE8jpUW</recordid><startdate>199502</startdate><enddate>199502</enddate><creator>Vile, John M.</creator><creator>D'Souza, Ursula M.</creator><creator>Strange, Philip G.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199502</creationdate><title>[3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions</title><author>Vile, John M. ; D'Souza, Ursula M. ; Strange, Philip G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2110-781ab92ba5125593b688172471f0e6bcd604b19fcc453dd9f337e89dfa45c87b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antagonist binding</topic><topic>Benzamides - metabolism</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>D2 dopamine receptor</topic><topic>D3 dopamine receptor</topic><topic>Dopamine Antagonists - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</topic><topic>Rats</topic><topic>Receptors, Dopamine - metabolism</topic><topic>Receptors, Dopamine D2 - metabolism</topic><topic>Receptors, Dopamine D3</topic><topic>Recombination, Genetic</topic><topic>Spiperone - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vile, John M.</creatorcontrib><creatorcontrib>D'Souza, Ursula M.</creatorcontrib><creatorcontrib>Strange, Philip G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vile, John M.</au><au>D'Souza, Ursula M.</au><au>Strange, Philip G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1995-02</date><risdate>1995</risdate><volume>64</volume><issue>2</issue><spage>940</spage><epage>943</epage><pages>940-943</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of <20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [3H]spiperone and [3H]nemonapride do not differentiate between different forms or populations of D2‐like receptors.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>7830089</pmid><doi>10.1046/j.1471-4159.1995.64020940.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1995-02, Vol.64 (2), p.940-943 |
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| subjects | Animals Antagonist binding Benzamides - metabolism Binding Sites Binding, Competitive Biological and medical sciences Cell receptors Cell structures and functions CHO Cells Cricetinae D2 dopamine receptor D3 dopamine receptor Dopamine Antagonists - metabolism Fundamental and applied biological sciences. Psychology Molecular and cellular biology Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Rats Receptors, Dopamine - metabolism Receptors, Dopamine D2 - metabolism Receptors, Dopamine D3 Recombination, Genetic Spiperone - metabolism Tritium |
| title | [3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions |
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