[3H]Nemonapride and [3H]Spiperone Label Equivalent Numbers of D2 and D3 Dopamine Receptors in a Range of Tissues and Under Different Conditions

: [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We...

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Veröffentlicht in:	Journal of neurochemistry 1995-02, Vol.64 (2), p.940-943
Hauptverfasser:	Vile, John M., D'Souza, Ursula M., Strange, Philip G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antagonist binding Benzamides - metabolism Binding Sites Binding, Competitive Biological and medical sciences Cell receptors Cell structures and functions CHO Cells Cricetinae D2 dopamine receptor D3 dopamine receptor Dopamine Antagonists - metabolism Fundamental and applied biological sciences. Psychology Molecular and cellular biology Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Rats Receptors, Dopamine - metabolism Receptors, Dopamine D2 - metabolism Receptors, Dopamine D3 Recombination, Genetic Spiperone - metabolism Tritium
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Zusammenfassung:	: [3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of
ISSN:	0022-3042 1471-4159
DOI:	10.1046/j.1471-4159.1995.64020940.x