Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine
The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli 015 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the s...
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| Veröffentlicht in: | The Journal of infectious diseases 1986-11, Vol.154 (5), p.742-751 |
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| container_title | The Journal of infectious diseases |
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| creator | Inman, L. R. Cantey, J. R. Formal, S. B. |
| description | The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli 015 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the O15 somatic antigen were compared for virulence in rabbits. The 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (P < .001). In fluorescent antibody- stained, frozen, sectioned tissues, fewer 7482-1-1 bacteria adhered to ileum (P = .013) and cecum (P = .044). Light and electron microscopic studies demonstrated that the recombinant strain, which adhered to membranous (M) cells to the same degree as the 7482-1-7 strain, was found beneath the epithelium, where its presence resulted in a marked acute inflammatory response. Both the 7482-1-1 and 7482-1-7 strains exhibited characteristic close adherence to M cells and absorptive ileal and cecal mucosa. |
| doi_str_mv | 10.1093/infdis/154.5.742 |
| format | Article |
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R. ; Cantey, J. R. ; Formal, S. B.</creator><creatorcontrib>Inman, L. R. ; Cantey, J. R. ; Formal, S. B.</creatorcontrib><description>The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli 015 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the O15 somatic antigen were compared for virulence in rabbits. The 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (P < .001). In fluorescent antibody- stained, frozen, sectioned tissues, fewer 7482-1-1 bacteria adhered to ileum (P = .013) and cecum (P = .044). Light and electron microscopic studies demonstrated that the recombinant strain, which adhered to membranous (M) cells to the same degree as the 7482-1-7 strain, was found beneath the epithelium, where its presence resulted in a marked acute inflammatory response. Both the 7482-1-1 and 7482-1-7 strains exhibited characteristic close adherence to M cells and absorptive ileal and cecal mucosa.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/154.5.742</identifier><identifier>PMID: 2430026</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Antigens, Bacterial - biosynthesis ; Bacteria ; Bacteriology ; Biological and medical sciences ; Cell Adhesion ; Diarrhea ; Epithelial cells ; Epithelium ; Escherichia coli ; Escherichia coli - immunology ; Escherichia coli - metabolism ; Escherichia coli - pathogenicity ; Fluorescent Antibody Technique ; Follicles ; Fundamental and applied biological sciences. Psychology ; Inoculation ; Intestinal Mucosa - microbiology ; Intestines - microbiology ; Lipopolysaccharides - analysis ; Lymph ; Microbiology ; Microscopy, Electron ; O Antigens ; Original Articles ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Peyers patches ; Rabbits ; Recombination, Genetic ; Shigella flexneri - immunology ; Somatic antigens</subject><ispartof>The Journal of infectious diseases, 1986-11, Vol.154 (5), p.742-751</ispartof><rights>Copyright 1986 The University of Chicago</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-60e3a7e49dde50b125247495afa481211b8957aae8641c3d91fb65000f48ec8b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30106688$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30106688$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8249986$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2430026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inman, L. R.</creatorcontrib><creatorcontrib>Cantey, J. R.</creatorcontrib><creatorcontrib>Formal, S. B.</creatorcontrib><title>Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli 015 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the O15 somatic antigen were compared for virulence in rabbits. The 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (P < .001). In fluorescent antibody- stained, frozen, sectioned tissues, fewer 7482-1-1 bacteria adhered to ileum (P = .013) and cecum (P = .044). Light and electron microscopic studies demonstrated that the recombinant strain, which adhered to membranous (M) cells to the same degree as the 7482-1-7 strain, was found beneath the epithelium, where its presence resulted in a marked acute inflammatory response. Both the 7482-1-1 and 7482-1-7 strains exhibited characteristic close adherence to M cells and absorptive ileal and cecal mucosa.</description><subject>Animals</subject><subject>Antigens, Bacterial - biosynthesis</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Diarrhea</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Escherichia coli</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli - pathogenicity</subject><subject>Fluorescent Antibody Technique</subject><subject>Follicles</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inoculation</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Intestines - microbiology</subject><subject>Lipopolysaccharides - analysis</subject><subject>Lymph</subject><subject>Microbiology</subject><subject>Microscopy, Electron</subject><subject>O Antigens</subject><subject>Original Articles</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Peyers patches</subject><subject>Rabbits</subject><subject>Recombination, Genetic</subject><subject>Shigella flexneri - immunology</subject><subject>Somatic antigens</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUtvEzEUhUcIVNLCng2SFwgVqZPaY48fy2oYaKUiRMJLbCyPx5O4TOxge6TC7-GH4pAQVpbud-45Vz5F8QzBOYICX1o39DZeoprM6zkj1YNihmrMSkoRfljMIKyqEnEhHhenMd5BCAmm7KQ4qQjOjM6K340fvbO_VLLeXYDPNkyjcdpcAOV68G7SPqoR3LhkgtI7DfBDRqDdTfxWpbVfGWc1aKNem2D12iqg_WjB-TIFZR1YvG6bEr0C7f02mBitW4Hl2q7MOCqw9JscrMGVS3niQJantQEL1XU2_U2NyTrzpHg0qDGap4f3rPj0pv3YXJe379_eNFe3pcYcp5JCgxUzRPS9qWGHqroijIhaDYpwVCHUcVEzpQynBGncCzR0tM6fMhBuNO_wWfFy77sN_seUs-XGRr271Bk_RckYQoJCmoVwL9TBxxjMILfBblT4KRGUu2LkvhiZi5G1zMXklecH76nbmP64cGgi8xcHrqJW4xCU09ngn4xXRAhO_9vcxeTDEWOIIKWcZ17uuY3J3B-5Ct8lZZjV8vrrN_lh0eCq-UIlxX8ASnqxdQ</recordid><startdate>19861101</startdate><enddate>19861101</enddate><creator>Inman, L. R.</creator><creator>Cantey, J. R.</creator><creator>Formal, S. B.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861101</creationdate><title>Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine</title><author>Inman, L. R. ; Cantey, J. R. ; Formal, S. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-60e3a7e49dde50b125247495afa481211b8957aae8641c3d91fb65000f48ec8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - biosynthesis</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion</topic><topic>Diarrhea</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Escherichia coli</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli - pathogenicity</topic><topic>Fluorescent Antibody Technique</topic><topic>Follicles</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inoculation</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Intestines - microbiology</topic><topic>Lipopolysaccharides - analysis</topic><topic>Lymph</topic><topic>Microbiology</topic><topic>Microscopy, Electron</topic><topic>O Antigens</topic><topic>Original Articles</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Peyers patches</topic><topic>Rabbits</topic><topic>Recombination, Genetic</topic><topic>Shigella flexneri - immunology</topic><topic>Somatic antigens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inman, L. R.</creatorcontrib><creatorcontrib>Cantey, J. R.</creatorcontrib><creatorcontrib>Formal, S. B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inman, L. R.</au><au>Cantey, J. R.</au><au>Formal, S. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1986-11-01</date><risdate>1986</risdate><volume>154</volume><issue>5</issue><spage>742</spage><epage>751</epage><pages>742-751</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli 015 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the O15 somatic antigen were compared for virulence in rabbits. The 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (P < .001). In fluorescent antibody- stained, frozen, sectioned tissues, fewer 7482-1-1 bacteria adhered to ileum (P = .013) and cecum (P = .044). Light and electron microscopic studies demonstrated that the recombinant strain, which adhered to membranous (M) cells to the same degree as the 7482-1-7 strain, was found beneath the epithelium, where its presence resulted in a marked acute inflammatory response. Both the 7482-1-1 and 7482-1-7 strains exhibited characteristic close adherence to M cells and absorptive ileal and cecal mucosa.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>2430026</pmid><doi>10.1093/infdis/154.5.742</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Antigens, Bacterial - biosynthesis Bacteria Bacteriology Biological and medical sciences Cell Adhesion Diarrhea Epithelial cells Epithelium Escherichia coli Escherichia coli - immunology Escherichia coli - metabolism Escherichia coli - pathogenicity Fluorescent Antibody Technique Follicles Fundamental and applied biological sciences. Psychology Inoculation Intestinal Mucosa - microbiology Intestines - microbiology Lipopolysaccharides - analysis Lymph Microbiology Microscopy, Electron O Antigens Original Articles Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Peyers patches Rabbits Recombination, Genetic Shigella flexneri - immunology Somatic antigens |
| title | Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine |
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