Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster
A comparative developmental analysis was made of lipids from wild‐type and adipose60 (adp60) mutants of Drosophila melanogaster. The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and...
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| Veröffentlicht in: | The Journal of experimental zoology 1986-10, Vol.240 (1), p.95-104 |
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| description | A comparative developmental analysis was made of lipids from wild‐type and adipose60 (adp60) mutants of Drosophila melanogaster. The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and gas‐liquid chromatography. Total lipid content of mutant adults was approximately twice that of the wild‐type, but no genotypic differences in lipid content were seen in earlier developmental stages. No sexual dimorphism was observed in total lipid content, although fatty acid profiles revealed some sexual differences. Many stage‐specific differences in fatty acid profiles and lipid content were developmentally associated with each genotype. Mutants tended to retain the larval phenotype in lipid content and, to a lesser extent, in fatty acid profile. In comparison to wild‐type, mutants tended to have increased lipid saturation, especially in 16‐carbon fatty acids in mature adults and in 18:0 fatty acids in late larvae and pupae. No significant difference between the mutants and wild‐type appeared in the developmental profiles for 14:1 fatty acid isomers. Hence, adp60 does not alter the desaturation‐elongation pathway, a secondary pathway for fatty acid desaturation in Drosophila, which received support from this analysis. |
| doi_str_mv | 10.1002/jez.1402400112 |
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The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and gas‐liquid chromatography. Total lipid content of mutant adults was approximately twice that of the wild‐type, but no genotypic differences in lipid content were seen in earlier developmental stages. No sexual dimorphism was observed in total lipid content, although fatty acid profiles revealed some sexual differences. Many stage‐specific differences in fatty acid profiles and lipid content were developmentally associated with each genotype. Mutants tended to retain the larval phenotype in lipid content and, to a lesser extent, in fatty acid profile. In comparison to wild‐type, mutants tended to have increased lipid saturation, especially in 16‐carbon fatty acids in mature adults and in 18:0 fatty acids in late larvae and pupae. No significant difference between the mutants and wild‐type appeared in the developmental profiles for 14:1 fatty acid isomers. Hence, adp60 does not alter the desaturation‐elongation pathway, a secondary pathway for fatty acid desaturation in Drosophila, which received support from this analysis.</description><identifier>ISSN: 0022-104X</identifier><identifier>EISSN: 1097-010X</identifier><identifier>DOI: 10.1002/jez.1402400112</identifier><identifier>PMID: 3095486</identifier><identifier>CODEN: JEZOAO</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ACIDE GRAS ; ACIDOS GRASOS ; Animals ; Biological and medical sciences ; CHROMATOGRAPHIE ; CHROMATOGRAPHY ; Chromatography, Gas ; Chromatography, Thin Layer ; Classical genetics, quantitative genetics, hybrids ; CONTENIDO DE LIPIDOS ; CROMATOGRAFIA ; DROSOPHILA ; Drosophila melanogaster - analysis ; Drosophila melanogaster - genetics ; Drosophila melanogaster - growth & development ; FATTY ACIDS ; Fatty Acids - analysis ; Fatty Acids - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; GENOTIPOS ; GENOTYPE ; GENOTYPES ; Invertebrata ; Larva ; LIPID CONTENT ; Lipids - analysis ; Lipids - genetics ; Male ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; Pupa ; Sex Characteristics ; TENEUR EN LIPIDES</subject><ispartof>The Journal of experimental zoology, 1986-10, Vol.240 (1), p.95-104</ispartof><rights>Copyright © 1986 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1987 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjez.1402400112$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjez.1402400112$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7997363$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3095486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teague, B.D</creatorcontrib><creatorcontrib>Clark, A.G</creatorcontrib><creatorcontrib>Doane, W.W</creatorcontrib><title>Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster</title><title>The Journal of experimental zoology</title><addtitle>J. Exp. Zool</addtitle><description>A comparative developmental analysis was made of lipids from wild‐type and adipose60 (adp60) mutants of Drosophila melanogaster. The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and gas‐liquid chromatography. Total lipid content of mutant adults was approximately twice that of the wild‐type, but no genotypic differences in lipid content were seen in earlier developmental stages. No sexual dimorphism was observed in total lipid content, although fatty acid profiles revealed some sexual differences. Many stage‐specific differences in fatty acid profiles and lipid content were developmentally associated with each genotype. Mutants tended to retain the larval phenotype in lipid content and, to a lesser extent, in fatty acid profile. In comparison to wild‐type, mutants tended to have increased lipid saturation, especially in 16‐carbon fatty acids in mature adults and in 18:0 fatty acids in late larvae and pupae. No significant difference between the mutants and wild‐type appeared in the developmental profiles for 14:1 fatty acid isomers. Hence, adp60 does not alter the desaturation‐elongation pathway, a secondary pathway for fatty acid desaturation in Drosophila, which received support from this analysis.</description><subject>ACIDE GRAS</subject><subject>ACIDOS GRASOS</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CHROMATOGRAPHIE</subject><subject>CHROMATOGRAPHY</subject><subject>Chromatography, Gas</subject><subject>Chromatography, Thin Layer</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>CONTENIDO DE LIPIDOS</subject><subject>CROMATOGRAFIA</subject><subject>DROSOPHILA</subject><subject>Drosophila melanogaster - analysis</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - growth & development</subject><subject>FATTY ACIDS</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>GENOTIPOS</subject><subject>GENOTYPE</subject><subject>GENOTYPES</subject><subject>Invertebrata</subject><subject>Larva</subject><subject>LIPID CONTENT</subject><subject>Lipids - analysis</subject><subject>Lipids - genetics</subject><subject>Male</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>Pupa</subject><subject>Sex Characteristics</subject><subject>TENEUR EN LIPIDES</subject><issn>0022-104X</issn><issn>1097-010X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1v1DAQxS0EKkvhygEJKQfELWXsfDg-QtttQRUIlaorLmYSj4uLE4c4Cyx_PS672p5s6_2eNfMeY885HHEA8eaW_h7xEkQJwLl4wBYclMyBw-ohWyRA5BzK1WP2JMbbO6QCecAOClBV2dQL9u2EfpEPY0_DjD7DAf0mupgFm3k3OhMzO4U---28yefNSIkwGRo3hkg1ZP16xmH-j59MIYbxu_OY9eRxCDcYZ5qeskcWfaRnu_OQXS1Pvxyf5xefzt4fv73IbZGmz0lWJS_RggDoTFlYbqghWQvbphs2tq1KAmVsZ4SqWiOasq2NIo4ISG1dHLLX23_HKfxcU5x172JHPk1CYR21lGl3JVQCX-7AdduT0ePkepw2ehdJ0l_tdIwdejvh0Lm4x6RSsqiLhKktlqKhzV7moO9q0akWfV-L_nD69f6VvPnW61JCf_ZenH7oWhay0tcfz7Q4v_y8ere81svEv9jyFoPGmymNc3XZSJCVaop_rXGZ_Q</recordid><startdate>198610</startdate><enddate>198610</enddate><creator>Teague, B.D</creator><creator>Clark, A.G</creator><creator>Doane, W.W</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198610</creationdate><title>Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster</title><author>Teague, B.D ; Clark, A.G ; Doane, W.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3402-e75414af0200cd43f1de8e762fb1dea8fb54e09dfcd295bd284b6d9e1aa0aeb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>ACIDE GRAS</topic><topic>ACIDOS GRASOS</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CHROMATOGRAPHIE</topic><topic>CHROMATOGRAPHY</topic><topic>Chromatography, Gas</topic><topic>Chromatography, Thin Layer</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>CONTENIDO DE LIPIDOS</topic><topic>CROMATOGRAFIA</topic><topic>DROSOPHILA</topic><topic>Drosophila melanogaster - analysis</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth & development</topic><topic>FATTY ACIDS</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>GENOTIPOS</topic><topic>GENOTYPE</topic><topic>GENOTYPES</topic><topic>Invertebrata</topic><topic>Larva</topic><topic>LIPID CONTENT</topic><topic>Lipids - analysis</topic><topic>Lipids - genetics</topic><topic>Male</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>Pupa</topic><topic>Sex Characteristics</topic><topic>TENEUR EN LIPIDES</topic><toplevel>online_resources</toplevel><creatorcontrib>Teague, B.D</creatorcontrib><creatorcontrib>Clark, A.G</creatorcontrib><creatorcontrib>Doane, W.W</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of experimental zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teague, B.D</au><au>Clark, A.G</au><au>Doane, W.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster</atitle><jtitle>The Journal of experimental zoology</jtitle><addtitle>J. Exp. Zool</addtitle><date>1986-10</date><risdate>1986</risdate><volume>240</volume><issue>1</issue><spage>95</spage><epage>104</epage><pages>95-104</pages><issn>0022-104X</issn><eissn>1097-010X</eissn><coden>JEZOAO</coden><abstract>A comparative developmental analysis was made of lipids from wild‐type and adipose60 (adp60) mutants of Drosophila melanogaster. The lipid content and fatty acid profiles of late third instar larvae, pupae, and mature adults were characterized in methanol:chloroform extracts utilizing thin layer and gas‐liquid chromatography. Total lipid content of mutant adults was approximately twice that of the wild‐type, but no genotypic differences in lipid content were seen in earlier developmental stages. No sexual dimorphism was observed in total lipid content, although fatty acid profiles revealed some sexual differences. Many stage‐specific differences in fatty acid profiles and lipid content were developmentally associated with each genotype. Mutants tended to retain the larval phenotype in lipid content and, to a lesser extent, in fatty acid profile. In comparison to wild‐type, mutants tended to have increased lipid saturation, especially in 16‐carbon fatty acids in mature adults and in 18:0 fatty acids in late larvae and pupae. No significant difference between the mutants and wild‐type appeared in the developmental profiles for 14:1 fatty acid isomers. Hence, adp60 does not alter the desaturation‐elongation pathway, a secondary pathway for fatty acid desaturation in Drosophila, which received support from this analysis.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3095486</pmid><doi>10.1002/jez.1402400112</doi><tpages>10</tpages></addata></record> |
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| subjects | ACIDE GRAS ACIDOS GRASOS Animals Biological and medical sciences CHROMATOGRAPHIE CHROMATOGRAPHY Chromatography, Gas Chromatography, Thin Layer Classical genetics, quantitative genetics, hybrids CONTENIDO DE LIPIDOS CROMATOGRAFIA DROSOPHILA Drosophila melanogaster - analysis Drosophila melanogaster - genetics Drosophila melanogaster - growth & development FATTY ACIDS Fatty Acids - analysis Fatty Acids - genetics Female Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution GENOTIPOS GENOTYPE GENOTYPES Invertebrata Larva LIPID CONTENT Lipids - analysis Lipids - genetics Male MUTANT MUTANTES MUTANTS Mutation Pupa Sex Characteristics TENEUR EN LIPIDES |
| title | Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster |
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