Rabbit IgH sequences in appendix germinal centers: VH diversification by gene conversion-like and hypermutation mechanisms
Although the rabbit IgH locus contains approximately 100 VH genes, the majority of B cells rearrange VH1. To produce a primary repertoire containing a sufficient number of protective antibodies, rearranged VH 1-DH-JH sequences may diversify within rabbit B cells in an organ that functions like a chi...
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| Veröffentlicht in: | Immunity (Cambridge, Mass.) Mass.), 1994-11, Vol.1 (8), p.647-659 |
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| creator | Weinstein, Peter D. Anderson, Arthur O. Maget, Rose G. |
| description | Although the rabbit IgH locus contains approximately 100 VH genes, the majority of B cells rearrange VH1. To produce a primary repertoire containing a sufficient number of protective antibodies, rearranged VH 1-DH-JH sequences may diversify within rabbit B cells in an organ that functions like a chicken bursa, sheep ileal Peyer's patch, or both. It was suggested many years ago that the rabbit appendix could be a bursal equivalent. To reexamine this possibility, we analyzed rearranged heavy chain variable region sequences in B cells from light and dark zones of appendix germinal centers from 6-week-old rabbits. Our findings indicate that antibody diversification occurs by gene conversion-like and somatic hypermutation mechanisms in appendix germinal centers of young rabbits. |
| doi_str_mv | 10.1016/1074-7613(94)90036-1 |
| format | Article |
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To produce a primary repertoire containing a sufficient number of protective antibodies, rearranged VH 1-DH-JH sequences may diversify within rabbit B cells in an organ that functions like a chicken bursa, sheep ileal Peyer's patch, or both. It was suggested many years ago that the rabbit appendix could be a bursal equivalent. To reexamine this possibility, we analyzed rearranged heavy chain variable region sequences in B cells from light and dark zones of appendix germinal centers from 6-week-old rabbits. Our findings indicate that antibody diversification occurs by gene conversion-like and somatic hypermutation mechanisms in appendix germinal centers of young rabbits.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibody Diversity</subject><subject>Antibody Formation</subject><subject>Appendix - cytology</subject><subject>Appendix - physiology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Gene Conversion</subject><subject>Gene Rearrangement, B-Lymphocyte, Heavy Chain</subject><subject>Genes, Immunoglobulin</subject><subject>Lymphoid Tissue - physiology</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Rabbits</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><issn>1074-7613</issn><issn>1097-4180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EKm3hDUDyCdFDYBx7Y5tDJVQBW6kSEgKu1tiZtIbECXa2Ynl6ku6KIyeP9H_zW_Mx9kLAGwGieStAq0o3Qr626sICyKYSj9ipAKsrJQw8Xucj8pSdlfIDQKiNhRN2ohuA2tan7M8X9D7O_Pp2ywv92lEKVHhMHKeJUht_81vKQ0zY80Bpplze8e9b3sb7ZYxdDDjHMXG_X7hEPIzpIRhT1cefxDG1_G4_LRW7-UAOFO4wxTKUZ-xJh32h58f3nH37-OHr1ba6-fzp-ur9TRXkRs9VbbCBrjYG6lp3WnrvNcogOoXKKCmFwmDQYxdwowxYY3znpVFog6zDxspz9urQO-VxObDMboglUN9jonFXnNYCGlWbBVQHMOSxlEydm3IcMO-dALcqd6tPt_p0VrkH5U4say-P_Ts_UPtv6eh4yS8POS1H3kfKroS4em5jpjC7doz__-AvJGKSEQ</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Weinstein, Peter D.</creator><creator>Anderson, Arthur O.</creator><creator>Maget, Rose G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19941101</creationdate><title>Rabbit IgH sequences in appendix germinal centers: VH diversification by gene conversion-like and hypermutation mechanisms</title><author>Weinstein, Peter D. ; Anderson, Arthur O. ; Maget, Rose G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-28a60f2880227f73bbb7a3c1f4a4843314ac8abafca5480988bfb384a9c32c593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibody Diversity</topic><topic>Antibody Formation</topic><topic>Appendix - cytology</topic><topic>Appendix - physiology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Gene Conversion</topic><topic>Gene Rearrangement, B-Lymphocyte, Heavy Chain</topic><topic>Genes, Immunoglobulin</topic><topic>Lymphoid Tissue - physiology</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Rabbits</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weinstein, Peter D.</creatorcontrib><creatorcontrib>Anderson, Arthur O.</creatorcontrib><creatorcontrib>Maget, Rose G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunity (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weinstein, Peter D.</au><au>Anderson, Arthur O.</au><au>Maget, Rose G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rabbit IgH sequences in appendix germinal centers: VH diversification by gene conversion-like and hypermutation mechanisms</atitle><jtitle>Immunity (Cambridge, Mass.)</jtitle><addtitle>Immunity</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>1</volume><issue>8</issue><spage>647</spage><epage>659</epage><pages>647-659</pages><issn>1074-7613</issn><eissn>1097-4180</eissn><abstract>Although the rabbit IgH locus contains approximately 100 VH genes, the majority of B cells rearrange VH1. 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| ispartof | Immunity (Cambridge, Mass.), 1994-11, Vol.1 (8), p.647-659 |
| issn | 1074-7613 1097-4180 |
| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Antibody Diversity Antibody Formation Appendix - cytology Appendix - physiology B-Lymphocytes - metabolism Base Sequence Cloning, Molecular Gene Conversion Gene Rearrangement, B-Lymphocyte, Heavy Chain Genes, Immunoglobulin Lymphoid Tissue - physiology Models, Genetic Molecular Sequence Data Mutation Polymerase Chain Reaction Rabbits Sequence Alignment Sequence Analysis, DNA |
| title | Rabbit IgH sequences in appendix germinal centers: VH diversification by gene conversion-like and hypermutation mechanisms |
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