Reproductive capacity of the nucleus of the male gamete after completion of meiosis
Our purpose was to investigate the possibility of achieving fertilization and subsequent normal embryonic development by injecting round spermatid nuclei into rabbit oocytes. Two-to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 1994-08, Vol.11 (7), p.335-341 |
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| container_end_page | 341 |
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| container_issue | 7 |
| container_start_page | 335 |
| container_title | Journal of assisted reproduction and genetics |
| container_volume | 11 |
| creator | SOFIKITIS, N. V MIYAGAWA, I AGAPITOS, E PASYIANOS, P TODA, T HELLSTROM, W. J. G KAWAMURA, H |
| description | Our purpose was to investigate the possibility of achieving fertilization and subsequent normal embryonic development by injecting round spermatid nuclei into rabbit oocytes.
Two-to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop in vitro up to the expanding blastocyst stage or in vivo up to complete gestation.
The current findings show that the haploid set of chromosomes of round spermatid can pair with the chromosomes of the ootid to participate in complete fertilization and subsequent embryonic and fetal development. In addition, we suggest that postmeiotic modifications of the round spermatid are not required for the pairing of male gamete chromosomes with those of the ootid. |
| doi_str_mv | 10.1007/bf02214138 |
| format | Article |
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Two-to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop in vitro up to the expanding blastocyst stage or in vivo up to complete gestation.
The current findings show that the haploid set of chromosomes of round spermatid can pair with the chromosomes of the ootid to participate in complete fertilization and subsequent embryonic and fetal development. In addition, we suggest that postmeiotic modifications of the round spermatid are not required for the pairing of male gamete chromosomes with those of the ootid.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/bf02214138</identifier><identifier>PMID: 7795365</identifier><identifier>CODEN: JARGE4</identifier><language>eng</language><publisher>New York, NY: Kluwer/Plenum</publisher><subject>Animals ; Biological and medical sciences ; Cell Nucleus - physiology ; Cell Nucleus - ultrastructure ; Female ; Fertilization in Vitro - methods ; Fundamental and applied biological sciences. Psychology ; Male ; Mammalian male genital system ; Meiosis ; Microscopy, Confocal ; Microscopy, Electron ; Morphology. Physiology ; Oocytes - cytology ; Oocytes - physiology ; Oocytes - ultrastructure ; Rabbits ; Sperm-Ovum Interactions ; Spermatids - cytology ; Spermatids - physiology ; Spermatids - ultrastructure ; Vertebrates: reproduction</subject><ispartof>Journal of assisted reproduction and genetics, 1994-08, Vol.11 (7), p.335-341</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-f16f7aaa9ea4814a52974f6a4ddd05c6ff00466d618f66e1da862726851fccf63</citedby><cites>FETCH-LOGICAL-c377t-f16f7aaa9ea4814a52974f6a4ddd05c6ff00466d618f66e1da862726851fccf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3559100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7795365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOFIKITIS, N. V</creatorcontrib><creatorcontrib>MIYAGAWA, I</creatorcontrib><creatorcontrib>AGAPITOS, E</creatorcontrib><creatorcontrib>PASYIANOS, P</creatorcontrib><creatorcontrib>TODA, T</creatorcontrib><creatorcontrib>HELLSTROM, W. J. G</creatorcontrib><creatorcontrib>KAWAMURA, H</creatorcontrib><title>Reproductive capacity of the nucleus of the male gamete after completion of meiosis</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><description>Our purpose was to investigate the possibility of achieving fertilization and subsequent normal embryonic development by injecting round spermatid nuclei into rabbit oocytes.
Two-to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop in vitro up to the expanding blastocyst stage or in vivo up to complete gestation.
The current findings show that the haploid set of chromosomes of round spermatid can pair with the chromosomes of the ootid to participate in complete fertilization and subsequent embryonic and fetal development. In addition, we suggest that postmeiotic modifications of the round spermatid are not required for the pairing of male gamete chromosomes with those of the ootid.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - physiology</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Meiosis</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron</subject><subject>Morphology. 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Physiology</topic><topic>Oocytes - cytology</topic><topic>Oocytes - physiology</topic><topic>Oocytes - ultrastructure</topic><topic>Rabbits</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatids - cytology</topic><topic>Spermatids - physiology</topic><topic>Spermatids - ultrastructure</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOFIKITIS, N. V</creatorcontrib><creatorcontrib>MIYAGAWA, I</creatorcontrib><creatorcontrib>AGAPITOS, E</creatorcontrib><creatorcontrib>PASYIANOS, P</creatorcontrib><creatorcontrib>TODA, T</creatorcontrib><creatorcontrib>HELLSTROM, W. J. 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Two-to four-cell-stage embryos developed after round spermatid nuclear injections into rabbit ooplasma could further develop in vitro up to the expanding blastocyst stage or in vivo up to complete gestation.
The current findings show that the haploid set of chromosomes of round spermatid can pair with the chromosomes of the ootid to participate in complete fertilization and subsequent embryonic and fetal development. In addition, we suggest that postmeiotic modifications of the round spermatid are not required for the pairing of male gamete chromosomes with those of the ootid.</abstract><cop>New York, NY</cop><pub>Kluwer/Plenum</pub><pmid>7795365</pmid><doi>10.1007/bf02214138</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1058-0468 |
| ispartof | Journal of assisted reproduction and genetics, 1994-08, Vol.11 (7), p.335-341 |
| issn | 1058-0468 1573-7330 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Cell Nucleus - physiology Cell Nucleus - ultrastructure Female Fertilization in Vitro - methods Fundamental and applied biological sciences. Psychology Male Mammalian male genital system Meiosis Microscopy, Confocal Microscopy, Electron Morphology. Physiology Oocytes - cytology Oocytes - physiology Oocytes - ultrastructure Rabbits Sperm-Ovum Interactions Spermatids - cytology Spermatids - physiology Spermatids - ultrastructure Vertebrates: reproduction |
| title | Reproductive capacity of the nucleus of the male gamete after completion of meiosis |
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