Nonequilibrium of two redox couplets in human plasma: Lactate-pyruvate and beta-hydroxybutyrate-acetoacetate
We examined the extracellular equilibrium status of two redox couplets normally found in plasma (lactatepyruvate [L-P], beta-hydroxybutyrate, and acetoacetate) during acute metabolic acidosis produced by muscle exertion. Both pre- and postexertion plasma spontaneously underwent loss of acetoacetate...
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| Veröffentlicht in: | Critical care medicine 1986-11, Vol.14 (11), p.936-941 |
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| creator | DAVIES, ALBERT O SAMUELSON, WAYNE M |
| description | We examined the extracellular equilibrium status of two redox couplets normally found in plasma (lactatepyruvate [L-P], beta-hydroxybutyrate, and acetoacetate) during acute metabolic acidosis produced by muscle exertion. Both pre- and postexertion plasma spontaneously underwent loss of acetoacetate and gain of L when compared to the baseline values. Exercise further induced a 332% rise in L (p < .001) and a 102% rise in P (p < .001). The empirically derived ratio of equilibrium constants, KI.P/KBA, fell 50% (p < .001), and the calculated change in free energy (ΔF) fell from 3.6 to 3.1 kcal/mol (p < .001) after exercise. The changes induced by exertion were simulated closely by an in vitro model of a reduced state. Thus, the triad of a) inconstant metabolite concentrations, b) inconstant KI.P/KBA, and c) ΔF both inconstant and non-zero, indicates that there is no state of equilibrium for these metabolite couplets in human plasma. The KI.P/KBA ratio appeared to reflect the degree of deviation from equilibrium and may therefore be useful when investigating altered redox states such as metabolic acidosis. |
| doi_str_mv | 10.1097/00003246-198611000-00005 |
| format | Article |
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Both pre- and postexertion plasma spontaneously underwent loss of acetoacetate and gain of L when compared to the baseline values. Exercise further induced a 332% rise in L (p < .001) and a 102% rise in P (p < .001). The empirically derived ratio of equilibrium constants, KI.P/KBA, fell 50% (p < .001), and the calculated change in free energy (ΔF) fell from 3.6 to 3.1 kcal/mol (p < .001) after exercise. The changes induced by exertion were simulated closely by an in vitro model of a reduced state. Thus, the triad of a) inconstant metabolite concentrations, b) inconstant KI.P/KBA, and c) ΔF both inconstant and non-zero, indicates that there is no state of equilibrium for these metabolite couplets in human plasma. The KI.P/KBA ratio appeared to reflect the degree of deviation from equilibrium and may therefore be useful when investigating altered redox states such as metabolic acidosis.</description><identifier>ISSN: 0090-3493</identifier><identifier>EISSN: 1530-0293</identifier><identifier>DOI: 10.1097/00003246-198611000-00005</identifier><identifier>PMID: 3769505</identifier><identifier>CODEN: CCMDC7</identifier><language>eng</language><publisher>Hagerstown, MD: Williams & Wilkins</publisher><subject>3-Hydroxybutyric Acid ; Acetoacetates - blood ; Acidosis - metabolism ; Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Emergency and intensive care: metabolism and nutrition disorders. Enteral and parenteral nutrition ; Female ; Humans ; Hydroxybutyrates - blood ; Hydroxybutyrates - metabolism ; Intensive care medicine ; Lactates - blood ; Lactates - metabolism ; Male ; Medical sciences ; NAD - blood ; NAD - metabolism ; Oxidation-Reduction ; Physical Exertion ; Pyruvates - blood ; Pyruvates - metabolism</subject><ispartof>Critical care medicine, 1986-11, Vol.14 (11), p.936-941</ispartof><rights>Williams & Wilkins 1986. All Rights Reserved.</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7945025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3769505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAVIES, ALBERT O</creatorcontrib><creatorcontrib>SAMUELSON, WAYNE M</creatorcontrib><title>Nonequilibrium of two redox couplets in human plasma: Lactate-pyruvate and beta-hydroxybutyrate-acetoacetate</title><title>Critical care medicine</title><addtitle>Crit Care Med</addtitle><description>We examined the extracellular equilibrium status of two redox couplets normally found in plasma (lactatepyruvate [L-P], beta-hydroxybutyrate, and acetoacetate) during acute metabolic acidosis produced by muscle exertion. Both pre- and postexertion plasma spontaneously underwent loss of acetoacetate and gain of L when compared to the baseline values. Exercise further induced a 332% rise in L (p < .001) and a 102% rise in P (p < .001). The empirically derived ratio of equilibrium constants, KI.P/KBA, fell 50% (p < .001), and the calculated change in free energy (ΔF) fell from 3.6 to 3.1 kcal/mol (p < .001) after exercise. The changes induced by exertion were simulated closely by an in vitro model of a reduced state. Thus, the triad of a) inconstant metabolite concentrations, b) inconstant KI.P/KBA, and c) ΔF both inconstant and non-zero, indicates that there is no state of equilibrium for these metabolite couplets in human plasma. The KI.P/KBA ratio appeared to reflect the degree of deviation from equilibrium and may therefore be useful when investigating altered redox states such as metabolic acidosis.</description><subject>3-Hydroxybutyric Acid</subject><subject>Acetoacetates - blood</subject><subject>Acidosis - metabolism</subject><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Emergency and intensive care: metabolism and nutrition disorders. Enteral and parenteral nutrition</subject><subject>Female</subject><subject>Humans</subject><subject>Hydroxybutyrates - blood</subject><subject>Hydroxybutyrates - metabolism</subject><subject>Intensive care medicine</subject><subject>Lactates - blood</subject><subject>Lactates - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>NAD - blood</subject><subject>NAD - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Physical Exertion</subject><subject>Pyruvates - blood</subject><subject>Pyruvates - metabolism</subject><issn>0090-3493</issn><issn>1530-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAUhS0EKkPLT0DyArEL2LGdxOxQxUsatRu6jm6cG03AiVM_mObf4zDD7PDC1-fc79rSMSGUs_ec6foDy0uUsiq4birOsyo2Sz0jO65EFqUWz8mOMc0KIbV4SV6F8JMxLlUtrsiVqCutmNoRe-dmfEyjHTs_pom6gcajox5790SNS4vFGOg400OaYKaLhTDBR7oHEyFisaw-_c4HCnNPO4xQHNbeu6e1S3H1GwEGo9u2LG7IiwFswNfnek0evnz-cfut2N9__X77aV-YUkpVIO-1UjV2veqgr0ollTDAWVMJ2StAAWUpZCO6LteyNroE6ORQMwWswexdk3enexfvHhOG2E5jMGgtzOhSaOua6UZpncHmBBrvQvA4tIsfJ_Bry1m7Bd3-C7q9BP3XUnn0zfmN1E3YXwbPyeb-23MfggE7eJjNGC5YraVi5YbJE3Z0NqIPv2w6om8PCDYe2v99s_gDxh-WoQ</recordid><startdate>198611</startdate><enddate>198611</enddate><creator>DAVIES, ALBERT O</creator><creator>SAMUELSON, WAYNE M</creator><general>Williams & Wilkins</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198611</creationdate><title>Nonequilibrium of two redox couplets in human plasma: Lactate-pyruvate and beta-hydroxybutyrate-acetoacetate</title><author>DAVIES, ALBERT O ; SAMUELSON, WAYNE M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2445-e1d9557ebd5bad625453ca108634d5ae3a223483bb22327c92aab4f705a08e223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>3-Hydroxybutyric Acid</topic><topic>Acetoacetates - blood</topic><topic>Acidosis - metabolism</topic><topic>Adult</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Emergency and intensive care: metabolism and nutrition disorders. Enteral and parenteral nutrition</topic><topic>Female</topic><topic>Humans</topic><topic>Hydroxybutyrates - blood</topic><topic>Hydroxybutyrates - metabolism</topic><topic>Intensive care medicine</topic><topic>Lactates - blood</topic><topic>Lactates - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>NAD - blood</topic><topic>NAD - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Physical Exertion</topic><topic>Pyruvates - blood</topic><topic>Pyruvates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAVIES, ALBERT O</creatorcontrib><creatorcontrib>SAMUELSON, WAYNE M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAVIES, ALBERT O</au><au>SAMUELSON, WAYNE M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonequilibrium of two redox couplets in human plasma: Lactate-pyruvate and beta-hydroxybutyrate-acetoacetate</atitle><jtitle>Critical care medicine</jtitle><addtitle>Crit Care Med</addtitle><date>1986-11</date><risdate>1986</risdate><volume>14</volume><issue>11</issue><spage>936</spage><epage>941</epage><pages>936-941</pages><issn>0090-3493</issn><eissn>1530-0293</eissn><coden>CCMDC7</coden><abstract>We examined the extracellular equilibrium status of two redox couplets normally found in plasma (lactatepyruvate [L-P], beta-hydroxybutyrate, and acetoacetate) during acute metabolic acidosis produced by muscle exertion. Both pre- and postexertion plasma spontaneously underwent loss of acetoacetate and gain of L when compared to the baseline values. Exercise further induced a 332% rise in L (p < .001) and a 102% rise in P (p < .001). The empirically derived ratio of equilibrium constants, KI.P/KBA, fell 50% (p < .001), and the calculated change in free energy (ΔF) fell from 3.6 to 3.1 kcal/mol (p < .001) after exercise. The changes induced by exertion were simulated closely by an in vitro model of a reduced state. Thus, the triad of a) inconstant metabolite concentrations, b) inconstant KI.P/KBA, and c) ΔF both inconstant and non-zero, indicates that there is no state of equilibrium for these metabolite couplets in human plasma. The KI.P/KBA ratio appeared to reflect the degree of deviation from equilibrium and may therefore be useful when investigating altered redox states such as metabolic acidosis.</abstract><cop>Hagerstown, MD</cop><pub>Williams & Wilkins</pub><pmid>3769505</pmid><doi>10.1097/00003246-198611000-00005</doi><tpages>6</tpages></addata></record> |
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| ispartof | Critical care medicine, 1986-11, Vol.14 (11), p.936-941 |
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| subjects | 3-Hydroxybutyric Acid Acetoacetates - blood Acidosis - metabolism Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Emergency and intensive care: metabolism and nutrition disorders. Enteral and parenteral nutrition Female Humans Hydroxybutyrates - blood Hydroxybutyrates - metabolism Intensive care medicine Lactates - blood Lactates - metabolism Male Medical sciences NAD - blood NAD - metabolism Oxidation-Reduction Physical Exertion Pyruvates - blood Pyruvates - metabolism |
| title | Nonequilibrium of two redox couplets in human plasma: Lactate-pyruvate and beta-hydroxybutyrate-acetoacetate |
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