Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs
A series of periodic increases in intracellular free calcium concentration ([Ca 2+] i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca 2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV c...
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| Veröffentlicht in: | Developmental biology 1986-11, Vol.118 (1), p.259-267 |
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| creator | Miyazaki, Shun-Ichi Hashimoto, Naohiro Yoshimoto, Yasuaki Kishimoto, Takeo Igusa, Yukio Hiramoto, Yukio |
| description | A series of periodic increases in intracellular free calcium concentration ([Ca
2+]
i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca
2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca
2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca
2+ rise. In the first response, which usually occurred 10–30 sec after the sperm attachment, the increase in [Ca
2+]
i began near the sperm attachment site, and the Ca
2+ rise spread over the entire egg within 4–7 sec. The Ca
2+ rise attained its peak in 5–8 sec, declined with almost even distribution, and ceased in 12–17 sec. The spreading Ca
2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (∼2 sec). In succeeding responses [Ca
2+]
i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca
2+ rise could take place from near the site of additional sperm attachment but only in the second or third response. |
| doi_str_mv | 10.1016/0012-1606(86)90093-X |
| format | Article |
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2+]
i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca
2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca
2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca
2+ rise. In the first response, which usually occurred 10–30 sec after the sperm attachment, the increase in [Ca
2+]
i began near the sperm attachment site, and the Ca
2+ rise spread over the entire egg within 4–7 sec. The Ca
2+ rise attained its peak in 5–8 sec, declined with almost even distribution, and ceased in 12–17 sec. The spreading Ca
2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (∼2 sec). In succeeding responses [Ca
2+]
i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca
2+ rise could take place from near the site of additional sperm attachment but only in the second or third response.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(86)90093-X</identifier><identifier>PMID: 3770302</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Aequorin ; Animals ; Biological and medical sciences ; Calcium - physiology ; Cell Compartmentation ; Cricetinae ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryology: invertebrates and vertebrates. Teratology ; Fertilization ; Fundamental and applied biological sciences. Psychology ; Male ; Ovum - physiology ; Periodicity ; Spermatozoa - physiology ; Time Factors ; Zygote - physiology</subject><ispartof>Developmental biology, 1986-11, Vol.118 (1), p.259-267</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-a7618bf8ea5e8eaf382a9edaec9b177576076961699a3c2593de77f52ede89c23</citedby><cites>FETCH-LOGICAL-c503t-a7618bf8ea5e8eaf382a9edaec9b177576076961699a3c2593de77f52ede89c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(86)90093-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8024888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3770302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyazaki, Shun-Ichi</creatorcontrib><creatorcontrib>Hashimoto, Naohiro</creatorcontrib><creatorcontrib>Yoshimoto, Yasuaki</creatorcontrib><creatorcontrib>Kishimoto, Takeo</creatorcontrib><creatorcontrib>Igusa, Yukio</creatorcontrib><creatorcontrib>Hiramoto, Yukio</creatorcontrib><title>Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>A series of periodic increases in intracellular free calcium concentration ([Ca
2+]
i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca
2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca
2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca
2+ rise. In the first response, which usually occurred 10–30 sec after the sperm attachment, the increase in [Ca
2+]
i began near the sperm attachment site, and the Ca
2+ rise spread over the entire egg within 4–7 sec. The Ca
2+ rise attained its peak in 5–8 sec, declined with almost even distribution, and ceased in 12–17 sec. The spreading Ca
2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (∼2 sec). In succeeding responses [Ca
2+]
i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca
2+ rise could take place from near the site of additional sperm attachment but only in the second or third response.</description><subject>Aequorin</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - physiology</subject><subject>Cell Compartmentation</subject><subject>Cricetinae</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fertilization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Ovum - physiology</subject><subject>Periodicity</subject><subject>Spermatozoa - physiology</subject><subject>Time Factors</subject><subject>Zygote - physiology</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo6zj6DxRyENFDa9KZzsdFkMUvWPCywtxCTVI9G-nutElaWPHHm3aGOQohKainXoonhDzn7C1nXL5jjLcNl0y-1vKNYcyIZv-AbDgzXdPJ3f4h2VyQx-RJzj8YY0JrcUWuhFJMsHZD_tziOMcEA4XJ0zxDCbX29xOMwWUae1rukM6YQvTB0TC5hJCxFvWUBA6HYRkg0T4hUgeDC8tIodAeUwlD-F0D47TmHOPgcaJ3MOaCieLxmJ-SRz0MGZ-d3y35_unj7fWX5ubb56_XH24a1zFRGlCS60OvETqsVy90CwY9oDMHrlSnJFPSSC6NAeHazgiPSvVdix61ca3Yklen3DnFnwvmYseQ181hwrhkW2UY3eoV3J1Al2LOCXs7pzBCurec2VW6XY3a1ajV0v6Tbvd17MU5fzmM6C9DZ8u1__Lch1wV9QkmF_IF06zd6fovW_L-hGF18StgstkFnBz6kNAV62P4_x5_AQasoCE</recordid><startdate>19861101</startdate><enddate>19861101</enddate><creator>Miyazaki, Shun-Ichi</creator><creator>Hashimoto, Naohiro</creator><creator>Yoshimoto, Yasuaki</creator><creator>Kishimoto, Takeo</creator><creator>Igusa, Yukio</creator><creator>Hiramoto, Yukio</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19861101</creationdate><title>Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs</title><author>Miyazaki, Shun-Ichi ; Hashimoto, Naohiro ; Yoshimoto, Yasuaki ; Kishimoto, Takeo ; Igusa, Yukio ; Hiramoto, Yukio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-a7618bf8ea5e8eaf382a9edaec9b177576076961699a3c2593de77f52ede89c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aequorin</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>Cell Compartmentation</topic><topic>Cricetinae</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fertilization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Ovum - physiology</topic><topic>Periodicity</topic><topic>Spermatozoa - physiology</topic><topic>Time Factors</topic><topic>Zygote - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyazaki, Shun-Ichi</creatorcontrib><creatorcontrib>Hashimoto, Naohiro</creatorcontrib><creatorcontrib>Yoshimoto, Yasuaki</creatorcontrib><creatorcontrib>Kishimoto, Takeo</creatorcontrib><creatorcontrib>Igusa, Yukio</creatorcontrib><creatorcontrib>Hiramoto, Yukio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyazaki, Shun-Ichi</au><au>Hashimoto, Naohiro</au><au>Yoshimoto, Yasuaki</au><au>Kishimoto, Takeo</au><au>Igusa, Yukio</au><au>Hiramoto, Yukio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1986-11-01</date><risdate>1986</risdate><volume>118</volume><issue>1</issue><spage>259</spage><epage>267</epage><pages>259-267</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>A series of periodic increases in intracellular free calcium concentration ([Ca
2+]
i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca
2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca
2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca
2+ rise. In the first response, which usually occurred 10–30 sec after the sperm attachment, the increase in [Ca
2+]
i began near the sperm attachment site, and the Ca
2+ rise spread over the entire egg within 4–7 sec. The Ca
2+ rise attained its peak in 5–8 sec, declined with almost even distribution, and ceased in 12–17 sec. The spreading Ca
2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (∼2 sec). In succeeding responses [Ca
2+]
i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca
2+ rise could take place from near the site of additional sperm attachment but only in the second or third response.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>3770302</pmid><doi>10.1016/0012-1606(86)90093-X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1986-11, Vol.118 (1), p.259-267 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77098282 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aequorin Animals Biological and medical sciences Calcium - physiology Cell Compartmentation Cricetinae Early stages. Segmentation. Gastrulation. Neurulation Embryology: invertebrates and vertebrates. Teratology Fertilization Fundamental and applied biological sciences. Psychology Male Ovum - physiology Periodicity Spermatozoa - physiology Time Factors Zygote - physiology |
| title | Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs |
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